Cargando…
Human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles
BACKGROUND: Breast cancer is a heterogeneous disease for which prognosis and treatment strategies are largely governed by the receptor status (estrogen, progesterone and Her2) of the tumor cells. Gene expression profiling of whole breast tumors further stratifies breast cancer into several molecular...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3505468/ https://www.ncbi.nlm.nih.gov/pubmed/22954256 http://dx.doi.org/10.1186/1755-8794-5-39 |
_version_ | 1782250760644329472 |
---|---|
author | Tchou, Julia Kossenkov, Andrew V Chang, Lisa Satija, Celine Herlyn, Meenhard Showe, Louise C Puré, Ellen |
author_facet | Tchou, Julia Kossenkov, Andrew V Chang, Lisa Satija, Celine Herlyn, Meenhard Showe, Louise C Puré, Ellen |
author_sort | Tchou, Julia |
collection | PubMed |
description | BACKGROUND: Breast cancer is a heterogeneous disease for which prognosis and treatment strategies are largely governed by the receptor status (estrogen, progesterone and Her2) of the tumor cells. Gene expression profiling of whole breast tumors further stratifies breast cancer into several molecular subtypes which also co-segregate with the receptor status of the tumor cells. We postulated that cancer associated fibroblasts (CAFs) within the tumor stroma may exhibit subtype specific gene expression profiles and thus contribute to the biology of the disease in a subtype specific manner. Several studies have reported gene expression profile differences between CAFs and normal breast fibroblasts but in none of these studies were the results stratified based on tumor subtypes. METHODS: To address whether gene expression in breast cancer associated fibroblasts varies between breast cancer subtypes, we compared the gene expression profiles of early passage primary CAFs isolated from twenty human breast cancer samples representing three main subtypes; seven ER+, seven triple negative (TNBC) and six Her2+. RESULTS: We observed significant expression differences between CAFs derived from Her2+ breast cancer and CAFs from TNBC and ER + cancers, particularly in pathways associated with cytoskeleton and integrin signaling. In the case of Her2+ breast cancer, the signaling pathways found to be selectively up regulated in CAFs likely contribute to the enhanced migration of breast cancer cells in transwell assays and may contribute to the unfavorable prognosis of Her2+ breast cancer. CONCLUSIONS: These data demonstrate that in addition to the distinct molecular profiles that characterize the neoplastic cells, CAF gene expression is also differentially regulated in distinct subtypes of breast cancer. |
format | Online Article Text |
id | pubmed-3505468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35054682012-11-25 Human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles Tchou, Julia Kossenkov, Andrew V Chang, Lisa Satija, Celine Herlyn, Meenhard Showe, Louise C Puré, Ellen BMC Med Genomics Research Article BACKGROUND: Breast cancer is a heterogeneous disease for which prognosis and treatment strategies are largely governed by the receptor status (estrogen, progesterone and Her2) of the tumor cells. Gene expression profiling of whole breast tumors further stratifies breast cancer into several molecular subtypes which also co-segregate with the receptor status of the tumor cells. We postulated that cancer associated fibroblasts (CAFs) within the tumor stroma may exhibit subtype specific gene expression profiles and thus contribute to the biology of the disease in a subtype specific manner. Several studies have reported gene expression profile differences between CAFs and normal breast fibroblasts but in none of these studies were the results stratified based on tumor subtypes. METHODS: To address whether gene expression in breast cancer associated fibroblasts varies between breast cancer subtypes, we compared the gene expression profiles of early passage primary CAFs isolated from twenty human breast cancer samples representing three main subtypes; seven ER+, seven triple negative (TNBC) and six Her2+. RESULTS: We observed significant expression differences between CAFs derived from Her2+ breast cancer and CAFs from TNBC and ER + cancers, particularly in pathways associated with cytoskeleton and integrin signaling. In the case of Her2+ breast cancer, the signaling pathways found to be selectively up regulated in CAFs likely contribute to the enhanced migration of breast cancer cells in transwell assays and may contribute to the unfavorable prognosis of Her2+ breast cancer. CONCLUSIONS: These data demonstrate that in addition to the distinct molecular profiles that characterize the neoplastic cells, CAF gene expression is also differentially regulated in distinct subtypes of breast cancer. BioMed Central 2012-09-06 /pmc/articles/PMC3505468/ /pubmed/22954256 http://dx.doi.org/10.1186/1755-8794-5-39 Text en Copyright ©2012 Tchou et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Tchou, Julia Kossenkov, Andrew V Chang, Lisa Satija, Celine Herlyn, Meenhard Showe, Louise C Puré, Ellen Human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles |
title | Human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles |
title_full | Human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles |
title_fullStr | Human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles |
title_full_unstemmed | Human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles |
title_short | Human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles |
title_sort | human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3505468/ https://www.ncbi.nlm.nih.gov/pubmed/22954256 http://dx.doi.org/10.1186/1755-8794-5-39 |
work_keys_str_mv | AT tchoujulia humanbreastcancerassociatedfibroblastsexhibitsubtypespecificgeneexpressionprofiles AT kossenkovandrewv humanbreastcancerassociatedfibroblastsexhibitsubtypespecificgeneexpressionprofiles AT changlisa humanbreastcancerassociatedfibroblastsexhibitsubtypespecificgeneexpressionprofiles AT satijaceline humanbreastcancerassociatedfibroblastsexhibitsubtypespecificgeneexpressionprofiles AT herlynmeenhard humanbreastcancerassociatedfibroblastsexhibitsubtypespecificgeneexpressionprofiles AT showelouisec humanbreastcancerassociatedfibroblastsexhibitsubtypespecificgeneexpressionprofiles AT pureellen humanbreastcancerassociatedfibroblastsexhibitsubtypespecificgeneexpressionprofiles |