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A one-step cloning method for the construction of somatic cell gene targeting vectors: application to production of human knockout cell lines

BACKGROUND: Gene targeting is a powerful method that can be used for examining the functions of genes. Traditionally, the construction of knockout (KO) vectors requires an amplification step to obtain two homologous, large fragments of genomic DNA. Restriction enzymes that cut at unique recognitions...

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Detalles Bibliográficos
Autores principales:	Liu, Yi, Li, Shangze, Zhang, Huihui, Wan, Zurong, Zhang, Xiaodong, Du, Runlei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3505732/ https://www.ncbi.nlm.nih.gov/pubmed/23046873 http://dx.doi.org/10.1186/1472-6750-12-71

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