Manipulation of gene expression in zebrafish using caged circular morpholino oligomers

Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs...

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Detalles Bibliográficos
Autores principales: Wang, Yuan, Wu, Li, Wang, Peng, Lv, Cong, Yang, Zhenjun, Tang, Xinjing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Synthetic Biology and Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3505977/
https://www.ncbi.nlm.nih.gov/pubmed/23002141
http://dx.doi.org/10.1093/nar/gks840
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author Wang, Yuan
Wu, Li
Wang, Peng
Lv, Cong
Yang, Zhenjun
Tang, Xinjing
author_facet Wang, Yuan
Wu, Li
Wang, Peng
Lv, Cong
Yang, Zhenjun
Tang, Xinjing
author_sort Wang, Yuan
collection PubMed
description Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs (caged cMOs) with two ends linked by a photocleavable moiety. These caged cMOs were successfully used to photomodulate β-catenin-2 and no tail expression in zebrafish embryos.
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spelling pubmed-35059772012-11-26 Manipulation of gene expression in zebrafish using caged circular morpholino oligomers Wang, Yuan Wu, Li Wang, Peng Lv, Cong Yang, Zhenjun Tang, Xinjing Nucleic Acids Res Synthetic Biology and Chemistry Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs (caged cMOs) with two ends linked by a photocleavable moiety. These caged cMOs were successfully used to photomodulate β-catenin-2 and no tail expression in zebrafish embryos. Oxford University Press 2012-11 2012-09-21 /pmc/articles/PMC3505977/ /pubmed/23002141 http://dx.doi.org/10.1093/nar/gks840 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Synthetic Biology and Chemistry
Wang, Yuan
Wu, Li
Wang, Peng
Lv, Cong
Yang, Zhenjun
Tang, Xinjing
Manipulation of gene expression in zebrafish using caged circular morpholino oligomers
title Manipulation of gene expression in zebrafish using caged circular morpholino oligomers
title_full Manipulation of gene expression in zebrafish using caged circular morpholino oligomers
title_fullStr Manipulation of gene expression in zebrafish using caged circular morpholino oligomers
title_full_unstemmed Manipulation of gene expression in zebrafish using caged circular morpholino oligomers
title_short Manipulation of gene expression in zebrafish using caged circular morpholino oligomers
title_sort manipulation of gene expression in zebrafish using caged circular morpholino oligomers
topic Synthetic Biology and Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3505977/
https://www.ncbi.nlm.nih.gov/pubmed/23002141
http://dx.doi.org/10.1093/nar/gks840
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