Fluorescent intercalator displacement replacement (FIDR) assay: determination of relative thermodynamic and kinetic parameters in triplex formation—a case study using triplex-forming LNAs

Triplex forming oligonucleotides (TFOs) are the most commonly used approach for site-specific targeting of double stranded DNA (dsDNA). Important parameters describing triplex formation include equilibrium binding constants (K(eq)) and association/dissociation rate constants (k(on) and k(off)). The...

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Detalles Bibliográficos
Autores principales: Sau, Sujay P., Kumar, Pawan, Sharma, Pawan K., Hrdlicka, Patrick J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3505983/
https://www.ncbi.nlm.nih.gov/pubmed/22855561
http://dx.doi.org/10.1093/nar/gks729

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