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Regeneration of mature dermis by transplanted particulate acellular dermal matrix in a rat model of skin defect wound

Native mammalian extracellular matrix (ECM) has been made in various forms including particles, sheet and mesh which are appropriate for site-specific applications. The ECM particles are usually created by homogenization method and have a wider size distribution. This needs to be improved to produce...

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Autores principales: Zuo, Haibin, Peng, Daizhi, Zheng, Bixiang, Liu, Xiaoling, Wang, Yong, Wang, Lihua, Zhou, Xin, Liu, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3506199/
https://www.ncbi.nlm.nih.gov/pubmed/22903602
http://dx.doi.org/10.1007/s10856-012-4745-9
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author Zuo, Haibin
Peng, Daizhi
Zheng, Bixiang
Liu, Xiaoling
Wang, Yong
Wang, Lihua
Zhou, Xin
Liu, Jing
author_facet Zuo, Haibin
Peng, Daizhi
Zheng, Bixiang
Liu, Xiaoling
Wang, Yong
Wang, Lihua
Zhou, Xin
Liu, Jing
author_sort Zuo, Haibin
collection PubMed
description Native mammalian extracellular matrix (ECM) has been made in various forms including particles, sheet and mesh which are appropriate for site-specific applications. The ECM particles are usually created by homogenization method and have a wider size distribution. This needs to be improved to produce more uniform ECM particles. In present study, we had successfully developed a method for preparing particulate acellular dermal matrix (PADM) in different gauges. The resultant PADM was approaching a rectangular parallelepiped or cubic shape, with a better or narrower size distribution than other ECM particles in previous reports. It also retained ultrastructure and functional molecules of native ECM. In vivo performances were evaluated after implantation of PADM in an acute full-thickness skin defect wound in rats. Histological analysis showed that allogeneic PADM used as dermal regeneration template could facilitate maturation and improving collagen bundle structure of regenerated dermis at the endpoint of 20 weeks post-surgery. The PADM could be used for further investigation in analyzing the impacts of cellularly and/or molecularly modified PADM on soft tissue regeneration.
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spelling pubmed-35061992012-11-28 Regeneration of mature dermis by transplanted particulate acellular dermal matrix in a rat model of skin defect wound Zuo, Haibin Peng, Daizhi Zheng, Bixiang Liu, Xiaoling Wang, Yong Wang, Lihua Zhou, Xin Liu, Jing J Mater Sci Mater Med Article Native mammalian extracellular matrix (ECM) has been made in various forms including particles, sheet and mesh which are appropriate for site-specific applications. The ECM particles are usually created by homogenization method and have a wider size distribution. This needs to be improved to produce more uniform ECM particles. In present study, we had successfully developed a method for preparing particulate acellular dermal matrix (PADM) in different gauges. The resultant PADM was approaching a rectangular parallelepiped or cubic shape, with a better or narrower size distribution than other ECM particles in previous reports. It also retained ultrastructure and functional molecules of native ECM. In vivo performances were evaluated after implantation of PADM in an acute full-thickness skin defect wound in rats. Histological analysis showed that allogeneic PADM used as dermal regeneration template could facilitate maturation and improving collagen bundle structure of regenerated dermis at the endpoint of 20 weeks post-surgery. The PADM could be used for further investigation in analyzing the impacts of cellularly and/or molecularly modified PADM on soft tissue regeneration. Springer US 2012-08-19 2012 /pmc/articles/PMC3506199/ /pubmed/22903602 http://dx.doi.org/10.1007/s10856-012-4745-9 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Zuo, Haibin
Peng, Daizhi
Zheng, Bixiang
Liu, Xiaoling
Wang, Yong
Wang, Lihua
Zhou, Xin
Liu, Jing
Regeneration of mature dermis by transplanted particulate acellular dermal matrix in a rat model of skin defect wound
title Regeneration of mature dermis by transplanted particulate acellular dermal matrix in a rat model of skin defect wound
title_full Regeneration of mature dermis by transplanted particulate acellular dermal matrix in a rat model of skin defect wound
title_fullStr Regeneration of mature dermis by transplanted particulate acellular dermal matrix in a rat model of skin defect wound
title_full_unstemmed Regeneration of mature dermis by transplanted particulate acellular dermal matrix in a rat model of skin defect wound
title_short Regeneration of mature dermis by transplanted particulate acellular dermal matrix in a rat model of skin defect wound
title_sort regeneration of mature dermis by transplanted particulate acellular dermal matrix in a rat model of skin defect wound
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3506199/
https://www.ncbi.nlm.nih.gov/pubmed/22903602
http://dx.doi.org/10.1007/s10856-012-4745-9
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