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Dexamethasone and vitamin B(12) synergistically promote peripheral nerve regeneration in rats by upregulating the expression of brain-derived neurotrophic factor

INTRODUCTION: Dexamethasone and vitamin B(12) are currently used in the clinic to treat peripheral nerve damage but their mechanisms of action remain incompletely understood. In this study we hypothesized that dexamethasone and vitamin B(12) promote the production of endogenous neurotrophic factors,...

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Autores principales: Sun, Hongzhi, Yang, Tao, Li, Qing, Zhu, Zhitu, Wang, Lei, Bai, Guang, Li, Dongsheng, Li, Qiang, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3506245/
https://www.ncbi.nlm.nih.gov/pubmed/23185205
http://dx.doi.org/10.5114/aoms.2012.31623
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author Sun, Hongzhi
Yang, Tao
Li, Qing
Zhu, Zhitu
Wang, Lei
Bai, Guang
Li, Dongsheng
Li, Qiang
Wang, Wei
author_facet Sun, Hongzhi
Yang, Tao
Li, Qing
Zhu, Zhitu
Wang, Lei
Bai, Guang
Li, Dongsheng
Li, Qiang
Wang, Wei
author_sort Sun, Hongzhi
collection PubMed
description INTRODUCTION: Dexamethasone and vitamin B(12) are currently used in the clinic to treat peripheral nerve damage but their mechanisms of action remain incompletely understood. In this study we hypothesized that dexamethasone and vitamin B(12) promote the production of endogenous neurotrophic factors, thereby enhancing peripheral nerve repair. MATERIAL AND METHODS: Ninety-six adult male Wistar rats were employed to establish a sciatic nerve injury model. They were then randomly divided into 4 groups to be subjected to different treatment: saline (group A), dexamethasone (group B), vitamin B(12) (group C), and dexamethasone combined with vitamin B(12) (group D). The walking behavior of rats was evaluated by footprint analysis, and the nerve regeneration was assessed by electrophysiological analysis and ultrastructural examination. The expression of brain-derived neurotrophic factor (BDNF), glial cell-derived neurotrophic factor, NT-3 and IL-6 in the injured sciatic nerves was detected by immunohistochemical and RT-PCR analysis. RESULTS: Dexamethasone and vitamin B(12) promoted the regeneration of myelinated nerve fibers and the proliferation of Schwann cells. Furthermore, dexamethasone and vitamin B(12) promoted the recovery of sciatic functional index and sensory nerve conduction velocity, and upregulated BDNF expression in the injured sciatic nerves. CONCLUSIONS: Dexamethasone and vitamin B(12) promote peripheral nerve repair in a rat model of sciatic nerve injury through the upregulation of BDNF expression. These findings provide new insight into the neurotrophic effects of dexamethasone and vitamin B(12) and support the application of these agents in clinical treatment of peripheral nerve injury.
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spelling pubmed-35062452012-11-26 Dexamethasone and vitamin B(12) synergistically promote peripheral nerve regeneration in rats by upregulating the expression of brain-derived neurotrophic factor Sun, Hongzhi Yang, Tao Li, Qing Zhu, Zhitu Wang, Lei Bai, Guang Li, Dongsheng Li, Qiang Wang, Wei Arch Med Sci Experimetal Research INTRODUCTION: Dexamethasone and vitamin B(12) are currently used in the clinic to treat peripheral nerve damage but their mechanisms of action remain incompletely understood. In this study we hypothesized that dexamethasone and vitamin B(12) promote the production of endogenous neurotrophic factors, thereby enhancing peripheral nerve repair. MATERIAL AND METHODS: Ninety-six adult male Wistar rats were employed to establish a sciatic nerve injury model. They were then randomly divided into 4 groups to be subjected to different treatment: saline (group A), dexamethasone (group B), vitamin B(12) (group C), and dexamethasone combined with vitamin B(12) (group D). The walking behavior of rats was evaluated by footprint analysis, and the nerve regeneration was assessed by electrophysiological analysis and ultrastructural examination. The expression of brain-derived neurotrophic factor (BDNF), glial cell-derived neurotrophic factor, NT-3 and IL-6 in the injured sciatic nerves was detected by immunohistochemical and RT-PCR analysis. RESULTS: Dexamethasone and vitamin B(12) promoted the regeneration of myelinated nerve fibers and the proliferation of Schwann cells. Furthermore, dexamethasone and vitamin B(12) promoted the recovery of sciatic functional index and sensory nerve conduction velocity, and upregulated BDNF expression in the injured sciatic nerves. CONCLUSIONS: Dexamethasone and vitamin B(12) promote peripheral nerve repair in a rat model of sciatic nerve injury through the upregulation of BDNF expression. These findings provide new insight into the neurotrophic effects of dexamethasone and vitamin B(12) and support the application of these agents in clinical treatment of peripheral nerve injury. Termedia Publishing House 2012-11-07 2012-11-09 /pmc/articles/PMC3506245/ /pubmed/23185205 http://dx.doi.org/10.5114/aoms.2012.31623 Text en Copyright © 2012 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Experimetal Research
Sun, Hongzhi
Yang, Tao
Li, Qing
Zhu, Zhitu
Wang, Lei
Bai, Guang
Li, Dongsheng
Li, Qiang
Wang, Wei
Dexamethasone and vitamin B(12) synergistically promote peripheral nerve regeneration in rats by upregulating the expression of brain-derived neurotrophic factor
title Dexamethasone and vitamin B(12) synergistically promote peripheral nerve regeneration in rats by upregulating the expression of brain-derived neurotrophic factor
title_full Dexamethasone and vitamin B(12) synergistically promote peripheral nerve regeneration in rats by upregulating the expression of brain-derived neurotrophic factor
title_fullStr Dexamethasone and vitamin B(12) synergistically promote peripheral nerve regeneration in rats by upregulating the expression of brain-derived neurotrophic factor
title_full_unstemmed Dexamethasone and vitamin B(12) synergistically promote peripheral nerve regeneration in rats by upregulating the expression of brain-derived neurotrophic factor
title_short Dexamethasone and vitamin B(12) synergistically promote peripheral nerve regeneration in rats by upregulating the expression of brain-derived neurotrophic factor
title_sort dexamethasone and vitamin b(12) synergistically promote peripheral nerve regeneration in rats by upregulating the expression of brain-derived neurotrophic factor
topic Experimetal Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3506245/
https://www.ncbi.nlm.nih.gov/pubmed/23185205
http://dx.doi.org/10.5114/aoms.2012.31623
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