Cargando…

Simulation of between Repeat Variability in Real Time PCR Reactions

While many decisions rely on real time quantitative PCR (qPCR) analysis few attempts have hitherto been made to quantify bounds of precision accounting for the various sources of variation involved in the measurement process. Besides influences of more obvious factors such as camera noise and pipett...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lievens, Antoon, Van Aelst, Stefan, Van den Bulcke, Marc, Goetghebeur, Els
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3506646/ https://www.ncbi.nlm.nih.gov/pubmed/23189123 http://dx.doi.org/10.1371/journal.pone.0047112

Ejemplares similares

Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR
por: Lievens, Antoon, et al.
Publicado: (2012)

A theoretical introduction to “Combinatory SYBR®Green qPCR Screening”, a matrix-based approach for the detection of materials derived from genetically modified plants
por: Van den Bulcke, Marc, et al.
Publicado: (2009)

Towards a Pathogenic Escherichia coli Detection Platform Using Multiplex SYBR®Green Real-Time PCR Methods and High Resolution Melting Analysis
por: Kagkli, Dafni-Maria, et al.
Publicado: (2012)

Evaluation of the efficacy of disinfectants against Puumala hantavirus by real-time RT-PCR
por: Maes, Piet, et al.
Publicado: (2007)

Impact of variance components on reliability of absolute quantification using digital PCR
por: Jacobs, Bart KM, et al.
Publicado: (2014)

The genetic structure of the Belgian population
por: Van den Eynden, Jimmy, et al.
Publicado: (2018)

Validating Real-Time Polymerase Chain Reaction (PCR) Assays
por: Smith, Melvyn
Publicado: (2021)

Viral load quantitation of SARS-coronavirus RNA using a one-step real-time RT-PCR
por: Keyaerts, Els, et al.
Publicado: (2006)

Detection and Quantification of Botanical Impurities in Commercial Oregano (Origanum vulgare) Using Metabarcoding and Digital PCR
por: Lievens, Antoon, et al.
Publicado: (2023)

New Method for Differentiation of Granuloviruses (Betabaculoviruses) Based on Real-Time Polymerase Chain Reaction (Real-Time PCR)
por: Krejmer-Rabalska, Martyna, et al.
Publicado: (2019)

Use of pJANUS™-02-001 as a calibrator plasmid for Roundup Ready soybean event GTS-40-3-2 detection: an interlaboratory trial assessment
por: Lievens, A., et al.
Publicado: (2009)

Quantitative Real-Time PCR
por: Sluijter, J. P. G., et al.
Publicado: (2006)

Estimating Real-Time qPCR Amplification Efficiency from Single-Reaction Data
por: Tellinghuisen, Joel
Publicado: (2021)

Low Utility of Repeat Real-Time PCR Testing for SARS-CoV-2 in Clinical Specimens
por: Challener, Douglas W., et al.
Publicado: (2020)

Diagnostic yield of repeat sampling with immunoassay, real-time PCR, and toxigenic culture for the detection of toxigenic Clostridium difficile in an epidemic and a non-epidemic setting
por: van Prehn, J., et al.
Publicado: (2015)

Evaluating hospital performance based on excess cause-specific incidence
por: Van Rompaye, Bart, et al.
Publicado: (2015)

Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability
por: Hilscher, Chelsey, et al.
Publicado: (2005)

Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR
por: Jacchia, Sara, et al.
Publicado: (2018)

Single-Reaction, Multiplex, Real-Time RT-PCR for the Detection, Quantitation, and Serotyping of Dengue Viruses
por: Waggoner, Jesse J., et al.
Publicado: (2013)

Real-time RT-PCR detection of 12 respiratory viral infections in four triplex reactions
por: Gunson, R.N., et al.
Publicado: (2005)

Single reaction, real time RT-PCR detection of all known avian and human metapneumoviruses
por: Lemaitre, E., et al.
Publicado: (2018)

Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data
por: Marullo, Manuela, et al.
Publicado: (2010)

Analytical Performance of Four Polymerase Chain Reaction (PCR) and Real Time PCR (qPCR) Assays for the Detection of Six Leishmania Species DNA in Colombia
por: León, Cielo M., et al.
Publicado: (2017)

The Effects of Repeated Testing, Simulated Malingering, and Traumatic Brain Injury on Visual Choice Reaction Time
por: Woods, David L., et al.
Publicado: (2015)

Mycoplasma hominis deep wound infection after neuromuscular scoliosis surgery: the use of real-time polymerase chain reaction (PCR)
por: Krijnen, Matthijs R., et al.
Publicado: (2006)

Real-Time PCR: An Essential Guide
por: McCaustland, Karen
Publicado: (2005)

Reference genes in real-time PCR
por: Kozera, Bartłomiej, et al.
Publicado: (2013)

Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR
por: Morey, Jeanine S., et al.
Publicado: (2006)

A Novel Multiplex Real-Time PCR for the Identification of Mycobacteria Associated with Zoonotic Tuberculosis
por: Reddington, Kate, et al.
Publicado: (2011)

Development of a Real-Time PCR for Identification of Brachyspira Species in Human Colonic Biopsies
por: Westerman, Laurens J., et al.
Publicado: (2012)

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
por: Six, Katrijn R., et al.
Publicado: (2017)

Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos
por: Goossens, Karen, et al.
Publicado: (2005)

2048. Comparison Between Endpoint and Real-Time (RT) Polymerase Chain Reaction (PCR) for the Diagnosis of Pneumocystis Pneumonia (PCP)
por: Zhou, Shiwei, et al.
Publicado: (2018)

Real -time PCR for early microbiological diagnosis: is it time?
por: Paiva, José-Artur, et al.
Publicado: (2017)

Clinical implications of rapid ePlex® Respiratory Pathogen Panel testing compared to laboratory-developed real-time PCR
por: van Rijn, Anneloes L., et al.
Publicado: (2017)

Effectiveness of Real-Time Quantitative PCR Compare to Repeat PCR for the Diagnosis of Charcot-Marie-Tooth Type 1A and Hereditary Neuropathy with Liability to Pressure Palsies
por: Choi, Jong Rak, et al.
Publicado: (2005)

Repeated measures of implementation variables
por: Fixsen, Dean L., et al.
Publicado: (2023)

Detection of respiratory pathogens by real-time PCR in children with clinical suspicion of pertussis
por: van Kruijssen, Alida M., et al.
Publicado: (2006)

Digital PCR dynamic range is approaching that of real-time quantitative PCR
por: Jones, Gerwyn M., et al.
Publicado: (2016)

Clinical and laboratory evaluation of a real-time PCR for Clostridium difficile toxin A and B genes
por: de Jong, E., et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_921ubm4qsv2o5bv6mmk6b15d46