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Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test
BACKGROUND: Gabapentin is thought to exert an effect through the voltage-dependent calcium channel. Vitamin E is a widely known antioxidant which neutralizes the harmful effect of ROS which is considered to play a prominent role in various painful conditions. This study was therefore conducted to as...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Anesthesiologists
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3506856/ https://www.ncbi.nlm.nih.gov/pubmed/23198040 http://dx.doi.org/10.4097/kjae.2012.63.5.447 |
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author | Kim, Myoung-Joong Lee, Won Hyung Ko, Young-Kwon Hong, Boo Hwi |
author_facet | Kim, Myoung-Joong Lee, Won Hyung Ko, Young-Kwon Hong, Boo Hwi |
author_sort | Kim, Myoung-Joong |
collection | PubMed |
description | BACKGROUND: Gabapentin is thought to exert an effect through the voltage-dependent calcium channel. Vitamin E is a widely known antioxidant which neutralizes the harmful effect of ROS which is considered to play a prominent role in various painful conditions. This study was therefore conducted to assess the antinociceptive effects of gabapentin and vitamin E and the interaction of these drugs in the modulation of pain in rats subjected to a formalin test. METHODS: Sprague-Dawley rats with a lumbar intrathecal catheter were tested for their paw flinches by 5% formalin injection after intrathecal injection of gabapentin or vitamin E. After obtaining dose-response curves for each drug, the effect of the combination was tested by the total dose fraction value and isobolographic analysis. RESULTS: When a single drug was injected intrathecally, significant dose-dependent decreases in flinches were shown only in the late phase. ED(50) values of intrathecal gabapentin and vitamin E in the late phase were 75.3 ± 9.58 µg, and 17.56 ± 1.65 mg/kg respectively. The combination of gabapentin and vitamin E produced dose-dependent decreases in the number of flinches in both phases induced by the formalin test. The ED(50) value of the combination was lower than the theoretical additive values in the late phase, but did not show a significant difference with the theoretical additive value. CONCLUSIONS: Gabapentin and vitamin E (by itself) have no antinociceptive effect in the early phase; however their combination has shown an antinociceptive effect. In addition, they show additive effects in the late phase of the formalin test. |
format | Online Article Text |
id | pubmed-3506856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Korean Society of Anesthesiologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-35068562012-11-29 Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test Kim, Myoung-Joong Lee, Won Hyung Ko, Young-Kwon Hong, Boo Hwi Korean J Anesthesiol Experimental Research Article BACKGROUND: Gabapentin is thought to exert an effect through the voltage-dependent calcium channel. Vitamin E is a widely known antioxidant which neutralizes the harmful effect of ROS which is considered to play a prominent role in various painful conditions. This study was therefore conducted to assess the antinociceptive effects of gabapentin and vitamin E and the interaction of these drugs in the modulation of pain in rats subjected to a formalin test. METHODS: Sprague-Dawley rats with a lumbar intrathecal catheter were tested for their paw flinches by 5% formalin injection after intrathecal injection of gabapentin or vitamin E. After obtaining dose-response curves for each drug, the effect of the combination was tested by the total dose fraction value and isobolographic analysis. RESULTS: When a single drug was injected intrathecally, significant dose-dependent decreases in flinches were shown only in the late phase. ED(50) values of intrathecal gabapentin and vitamin E in the late phase were 75.3 ± 9.58 µg, and 17.56 ± 1.65 mg/kg respectively. The combination of gabapentin and vitamin E produced dose-dependent decreases in the number of flinches in both phases induced by the formalin test. The ED(50) value of the combination was lower than the theoretical additive values in the late phase, but did not show a significant difference with the theoretical additive value. CONCLUSIONS: Gabapentin and vitamin E (by itself) have no antinociceptive effect in the early phase; however their combination has shown an antinociceptive effect. In addition, they show additive effects in the late phase of the formalin test. The Korean Society of Anesthesiologists 2012-11 2012-11-16 /pmc/articles/PMC3506856/ /pubmed/23198040 http://dx.doi.org/10.4097/kjae.2012.63.5.447 Text en Copyright © the Korean Society of Anesthesiologists, 2012 http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Experimental Research Article Kim, Myoung-Joong Lee, Won Hyung Ko, Young-Kwon Hong, Boo Hwi Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test |
title | Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test |
title_full | Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test |
title_fullStr | Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test |
title_full_unstemmed | Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test |
title_short | Antinociceptive drug interaction between intrathecal vitamin E and gabapentin in the rat formalin test |
title_sort | antinociceptive drug interaction between intrathecal vitamin e and gabapentin in the rat formalin test |
topic | Experimental Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3506856/ https://www.ncbi.nlm.nih.gov/pubmed/23198040 http://dx.doi.org/10.4097/kjae.2012.63.5.447 |
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