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Life-long preservation of the regenerative capacity in the fin and heart in zebrafish
The zebrafish is a widely used model animal to study the regeneration of organs, such as the fin and heart. Their average lifetime is about 3 years, and recent studies have shown that zebrafish exhibit aging-related degeneration, suggesting the possibility that aging might affect regenerative potent...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507221/ https://www.ncbi.nlm.nih.gov/pubmed/23213467 http://dx.doi.org/10.1242/bio.20121057 |
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author | Itou, Junji Kawakami, Hiroko Burgoyne, Tyler Kawakami, Yasuhiko |
author_facet | Itou, Junji Kawakami, Hiroko Burgoyne, Tyler Kawakami, Yasuhiko |
author_sort | Itou, Junji |
collection | PubMed |
description | The zebrafish is a widely used model animal to study the regeneration of organs, such as the fin and heart. Their average lifetime is about 3 years, and recent studies have shown that zebrafish exhibit aging-related degeneration, suggesting the possibility that aging might affect regenerative potential. In order to investigate this possibility, we compared regeneration of the fin and heart after experimental amputation in young (6–12 month old) and old (26–36 month old) fish. Comparison of recovery rate of the caudal fin, measured every two or three days from one day post amputation until 13 days post amputation, show that fins in young and old fish regenerate at a similar rate. In the heart, myocardium regeneration and cardiomyocyte proliferation occurred similarly in the two groups. Moreover, neo-vascularization, as well as activation of fibroblast growth factor signaling, which is required for neo-vascularization, occurred similarly. The epicardial tissue is a thin layer tissue that covers the heart, and starts to express several genes immediately in response to injury. The expression of epicardial genes, such as wt1b and aldh1a2, in response to heart injury was comparable in two groups. Our results demonstrate that zebrafish preserve a life-long regenerative ability of the caudal fin and heart. |
format | Online Article Text |
id | pubmed-3507221 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Company of Biologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-35072212012-12-04 Life-long preservation of the regenerative capacity in the fin and heart in zebrafish Itou, Junji Kawakami, Hiroko Burgoyne, Tyler Kawakami, Yasuhiko Biol Open Research Article The zebrafish is a widely used model animal to study the regeneration of organs, such as the fin and heart. Their average lifetime is about 3 years, and recent studies have shown that zebrafish exhibit aging-related degeneration, suggesting the possibility that aging might affect regenerative potential. In order to investigate this possibility, we compared regeneration of the fin and heart after experimental amputation in young (6–12 month old) and old (26–36 month old) fish. Comparison of recovery rate of the caudal fin, measured every two or three days from one day post amputation until 13 days post amputation, show that fins in young and old fish regenerate at a similar rate. In the heart, myocardium regeneration and cardiomyocyte proliferation occurred similarly in the two groups. Moreover, neo-vascularization, as well as activation of fibroblast growth factor signaling, which is required for neo-vascularization, occurred similarly. The epicardial tissue is a thin layer tissue that covers the heart, and starts to express several genes immediately in response to injury. The expression of epicardial genes, such as wt1b and aldh1a2, in response to heart injury was comparable in two groups. Our results demonstrate that zebrafish preserve a life-long regenerative ability of the caudal fin and heart. The Company of Biologists 2012-06-13 /pmc/articles/PMC3507221/ /pubmed/23213467 http://dx.doi.org/10.1242/bio.20121057 Text en © 2012. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by-nc-sa/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Article Itou, Junji Kawakami, Hiroko Burgoyne, Tyler Kawakami, Yasuhiko Life-long preservation of the regenerative capacity in the fin and heart in zebrafish |
title | Life-long preservation of the regenerative capacity in the fin and heart in zebrafish |
title_full | Life-long preservation of the regenerative capacity in the fin and heart in zebrafish |
title_fullStr | Life-long preservation of the regenerative capacity in the fin and heart in zebrafish |
title_full_unstemmed | Life-long preservation of the regenerative capacity in the fin and heart in zebrafish |
title_short | Life-long preservation of the regenerative capacity in the fin and heart in zebrafish |
title_sort | life-long preservation of the regenerative capacity in the fin and heart in zebrafish |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507221/ https://www.ncbi.nlm.nih.gov/pubmed/23213467 http://dx.doi.org/10.1242/bio.20121057 |
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