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Control of growth and inflammatory response of macrophages and foam cells with nanotopography

Macrophages play an important role in modulating the immune function of the human body, while foam cells differentiated from macrophages with subsequent fatty streak formation play a key role in atherosclerosis. We hypothesized that nanotopography modulates the behavior and function of macrophages a...

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Autores principales: Mohiuddin, Mohammed, Pan, Hsu-An, Hung, Yao-Ching, Huang, Guewha Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507756/
https://www.ncbi.nlm.nih.gov/pubmed/22799434
http://dx.doi.org/10.1186/1556-276X-7-394
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author Mohiuddin, Mohammed
Pan, Hsu-An
Hung, Yao-Ching
Huang, Guewha Steven
author_facet Mohiuddin, Mohammed
Pan, Hsu-An
Hung, Yao-Ching
Huang, Guewha Steven
author_sort Mohiuddin, Mohammed
collection PubMed
description Macrophages play an important role in modulating the immune function of the human body, while foam cells differentiated from macrophages with subsequent fatty streak formation play a key role in atherosclerosis. We hypothesized that nanotopography modulates the behavior and function of macrophages and foam cells without bioactive agent. In the present study, nanodot arrays ranging from 10‐ to 200‐nm were used to evaluate the growth and function of macrophages and foam cells. In the quantitative analysis, the cell adhesion area in macrophages increased with 10- to 50-nm nanodot arrays compared to the flat surface, while it decreased with 100- and 200-nm nanodot arrays. A similar trend of adhesion was observed in foam cells. Immunostaining, specific to vinculin and actin filaments, indicated that a 50-nm surface promoted cell adhesion and cytoskeleton organization. On the contrary, 200-nm surfaces hindered cell adhesion and cytoskeleton organization. Further, based on quantitative real-time polymerase chain reaction data, expression of inflammatory genes was upregulated for the 100- and 200-nm surfaces in macrophages and foam cells. This suggests that nanodots of 100‐ and 200‐nm triggered immune inflammatory stress response. In summary, nanotopography controls cell morphology, adhesions, and proliferation. By adjusting the nanodot diameter, we could modulate the growth and expression of function-related genes in the macrophages and foam cell system. The nanotopography-mediated control of cell growth and morphology provides potential insight for designing cardiovascular implants.
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spelling pubmed-35077562012-11-29 Control of growth and inflammatory response of macrophages and foam cells with nanotopography Mohiuddin, Mohammed Pan, Hsu-An Hung, Yao-Ching Huang, Guewha Steven Nanoscale Res Lett Nano Express Macrophages play an important role in modulating the immune function of the human body, while foam cells differentiated from macrophages with subsequent fatty streak formation play a key role in atherosclerosis. We hypothesized that nanotopography modulates the behavior and function of macrophages and foam cells without bioactive agent. In the present study, nanodot arrays ranging from 10‐ to 200‐nm were used to evaluate the growth and function of macrophages and foam cells. In the quantitative analysis, the cell adhesion area in macrophages increased with 10- to 50-nm nanodot arrays compared to the flat surface, while it decreased with 100- and 200-nm nanodot arrays. A similar trend of adhesion was observed in foam cells. Immunostaining, specific to vinculin and actin filaments, indicated that a 50-nm surface promoted cell adhesion and cytoskeleton organization. On the contrary, 200-nm surfaces hindered cell adhesion and cytoskeleton organization. Further, based on quantitative real-time polymerase chain reaction data, expression of inflammatory genes was upregulated for the 100- and 200-nm surfaces in macrophages and foam cells. This suggests that nanodots of 100‐ and 200‐nm triggered immune inflammatory stress response. In summary, nanotopography controls cell morphology, adhesions, and proliferation. By adjusting the nanodot diameter, we could modulate the growth and expression of function-related genes in the macrophages and foam cell system. The nanotopography-mediated control of cell growth and morphology provides potential insight for designing cardiovascular implants. Springer 2012-07-16 /pmc/articles/PMC3507756/ /pubmed/22799434 http://dx.doi.org/10.1186/1556-276X-7-394 Text en Copyright ©2012 Mohiuddin et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Nano Express
Mohiuddin, Mohammed
Pan, Hsu-An
Hung, Yao-Ching
Huang, Guewha Steven
Control of growth and inflammatory response of macrophages and foam cells with nanotopography
title Control of growth and inflammatory response of macrophages and foam cells with nanotopography
title_full Control of growth and inflammatory response of macrophages and foam cells with nanotopography
title_fullStr Control of growth and inflammatory response of macrophages and foam cells with nanotopography
title_full_unstemmed Control of growth and inflammatory response of macrophages and foam cells with nanotopography
title_short Control of growth and inflammatory response of macrophages and foam cells with nanotopography
title_sort control of growth and inflammatory response of macrophages and foam cells with nanotopography
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507756/
https://www.ncbi.nlm.nih.gov/pubmed/22799434
http://dx.doi.org/10.1186/1556-276X-7-394
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