Cargando…
Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011
Case reports of Apophysomyces spp. in immunocompetent hosts have been a result of traumatic deep implantation of Apophysomyces spp. spore-contaminated soil or debris. On May 22, 2011 a tornado occurred in Joplin, MO, leaving 13 tornado victims with Apophysomyces trapeziformis infections as a result...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507928/ https://www.ncbi.nlm.nih.gov/pubmed/23209631 http://dx.doi.org/10.1371/journal.pone.0049989 |
_version_ | 1782251165663100928 |
---|---|
author | Etienne, Kizee A. Gillece, John Hilsabeck, Remy Schupp, Jim M. Colman, Rebecca Lockhart, Shawn R. Gade, Lalitha Thompson, Elizabeth H. Sutton, Deanna A. Neblett-Fanfair, Robyn Park, Benjamin J. Turabelidze, George Keim, Paul Brandt, Mary E. Deak, Eszter Engelthaler, David M. |
author_facet | Etienne, Kizee A. Gillece, John Hilsabeck, Remy Schupp, Jim M. Colman, Rebecca Lockhart, Shawn R. Gade, Lalitha Thompson, Elizabeth H. Sutton, Deanna A. Neblett-Fanfair, Robyn Park, Benjamin J. Turabelidze, George Keim, Paul Brandt, Mary E. Deak, Eszter Engelthaler, David M. |
author_sort | Etienne, Kizee A. |
collection | PubMed |
description | Case reports of Apophysomyces spp. in immunocompetent hosts have been a result of traumatic deep implantation of Apophysomyces spp. spore-contaminated soil or debris. On May 22, 2011 a tornado occurred in Joplin, MO, leaving 13 tornado victims with Apophysomyces trapeziformis infections as a result of lacerations from airborne material. We used whole genome sequence typing (WGST) for high-resolution phylogenetic SNP analysis of 17 outbreak Apophysomyces isolates and five additional temporally and spatially diverse Apophysomyces control isolates (three A. trapeziformis and two A. variabilis isolates). Whole genome SNP phylogenetic analysis revealed three clusters of genotypically related or identical A. trapeziformis isolates and multiple distinct isolates among the Joplin group; this indicated multiple genotypes from a single or multiple sources. Though no linkage between genotype and location of exposure was observed, WGST analysis determined that the Joplin isolates were more closely related to each other than to the control isolates, suggesting local population structure. Additionally, species delineation based on WGST demonstrated the need to reassess currently accepted taxonomic classifications of phylogenetic species within the genus Apophysomyces. |
format | Online Article Text |
id | pubmed-3507928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35079282012-12-03 Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011 Etienne, Kizee A. Gillece, John Hilsabeck, Remy Schupp, Jim M. Colman, Rebecca Lockhart, Shawn R. Gade, Lalitha Thompson, Elizabeth H. Sutton, Deanna A. Neblett-Fanfair, Robyn Park, Benjamin J. Turabelidze, George Keim, Paul Brandt, Mary E. Deak, Eszter Engelthaler, David M. PLoS One Research Article Case reports of Apophysomyces spp. in immunocompetent hosts have been a result of traumatic deep implantation of Apophysomyces spp. spore-contaminated soil or debris. On May 22, 2011 a tornado occurred in Joplin, MO, leaving 13 tornado victims with Apophysomyces trapeziformis infections as a result of lacerations from airborne material. We used whole genome sequence typing (WGST) for high-resolution phylogenetic SNP analysis of 17 outbreak Apophysomyces isolates and five additional temporally and spatially diverse Apophysomyces control isolates (three A. trapeziformis and two A. variabilis isolates). Whole genome SNP phylogenetic analysis revealed three clusters of genotypically related or identical A. trapeziformis isolates and multiple distinct isolates among the Joplin group; this indicated multiple genotypes from a single or multiple sources. Though no linkage between genotype and location of exposure was observed, WGST analysis determined that the Joplin isolates were more closely related to each other than to the control isolates, suggesting local population structure. Additionally, species delineation based on WGST demonstrated the need to reassess currently accepted taxonomic classifications of phylogenetic species within the genus Apophysomyces. Public Library of Science 2012-11-27 /pmc/articles/PMC3507928/ /pubmed/23209631 http://dx.doi.org/10.1371/journal.pone.0049989 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Etienne, Kizee A. Gillece, John Hilsabeck, Remy Schupp, Jim M. Colman, Rebecca Lockhart, Shawn R. Gade, Lalitha Thompson, Elizabeth H. Sutton, Deanna A. Neblett-Fanfair, Robyn Park, Benjamin J. Turabelidze, George Keim, Paul Brandt, Mary E. Deak, Eszter Engelthaler, David M. Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011 |
title | Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011 |
title_full | Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011 |
title_fullStr | Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011 |
title_full_unstemmed | Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011 |
title_short | Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011 |
title_sort | whole genome sequence typing to investigate the apophysomyces outbreak following a tornado in joplin, missouri, 2011 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507928/ https://www.ncbi.nlm.nih.gov/pubmed/23209631 http://dx.doi.org/10.1371/journal.pone.0049989 |
work_keys_str_mv | AT etiennekizeea wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT gillecejohn wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT hilsabeckremy wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT schuppjimm wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT colmanrebecca wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT lockhartshawnr wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT gadelalitha wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT thompsonelizabethh wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT suttondeannaa wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT neblettfanfairrobyn wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT parkbenjaminj wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT turabelidzegeorge wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT keimpaul wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT brandtmarye wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT deakeszter wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 AT engelthalerdavidm wholegenomesequencetypingtoinvestigatetheapophysomycesoutbreakfollowingatornadoinjoplinmissouri2011 |