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Identification and characterization of microRNAs in white and brown alpaca skin

BACKGROUND: MicroRNAs (miRNAs) are small, non-coding 21–25 nt RNA molecules that play an important role in regulating gene expression. Little is known about the expression profiles and functions of miRNAs in skin and their role in pigmentation. Alpacas have more than 22 natural coat colors, more tha...

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Autores principales: Tian, Xue, Jiang, Junbing, Fan, Ruiwen, Wang, Haidong, Meng, Xiaolin, He, Xiaoyan, He, Junping, Li, Hongquan, Geng, Jianjun, Yu, Xiuju, Song, Yunfei, Zhang, Danli, Yao, Jianbo, Smith, George W, Dong, Changsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3508611/
https://www.ncbi.nlm.nih.gov/pubmed/23067000
http://dx.doi.org/10.1186/1471-2164-13-555
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author Tian, Xue
Jiang, Junbing
Fan, Ruiwen
Wang, Haidong
Meng, Xiaolin
He, Xiaoyan
He, Junping
Li, Hongquan
Geng, Jianjun
Yu, Xiuju
Song, Yunfei
Zhang, Danli
Yao, Jianbo
Smith, George W
Dong, Changsheng
author_facet Tian, Xue
Jiang, Junbing
Fan, Ruiwen
Wang, Haidong
Meng, Xiaolin
He, Xiaoyan
He, Junping
Li, Hongquan
Geng, Jianjun
Yu, Xiuju
Song, Yunfei
Zhang, Danli
Yao, Jianbo
Smith, George W
Dong, Changsheng
author_sort Tian, Xue
collection PubMed
description BACKGROUND: MicroRNAs (miRNAs) are small, non-coding 21–25 nt RNA molecules that play an important role in regulating gene expression. Little is known about the expression profiles and functions of miRNAs in skin and their role in pigmentation. Alpacas have more than 22 natural coat colors, more than any other fiber producing species. To better understand the role of miRNAs in control of coat color we performed a comprehensive analysis of miRNA expression profiles in skin of white versus brown alpacas. RESULTS: Two small RNA libraries from white alpaca (WA) and brown alpaca (BA) skin were sequenced with the aid of Illumina sequencing technology. 272 and 267 conserved miRNAs were obtained from the WA and BA skin libraries, respectively. Of these conserved miRNAs, 35 and 13 were more abundant in WA and BA skin, respectively. The targets of these miRNAs were predicted and grouped based on Gene Ontology and KEGG pathway analysis. Many predicted target genes for these miRNAs are involved in the melanogenesis pathway controlling pigmentation. In addition to the conserved miRNAs, we also obtained 22 potentially novel miRNAs from the WA and BA skin libraries. CONCLUSION: This study represents the first comprehensive survey of miRNAs expressed in skin of animals of different coat colors by deep sequencing analysis. We discovered a collection of miRNAs that are differentially expressed in WA and BA skin. The results suggest important potential functions of miRNAs in coat color regulation.
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spelling pubmed-35086112012-11-29 Identification and characterization of microRNAs in white and brown alpaca skin Tian, Xue Jiang, Junbing Fan, Ruiwen Wang, Haidong Meng, Xiaolin He, Xiaoyan He, Junping Li, Hongquan Geng, Jianjun Yu, Xiuju Song, Yunfei Zhang, Danli Yao, Jianbo Smith, George W Dong, Changsheng BMC Genomics Research Article BACKGROUND: MicroRNAs (miRNAs) are small, non-coding 21–25 nt RNA molecules that play an important role in regulating gene expression. Little is known about the expression profiles and functions of miRNAs in skin and their role in pigmentation. Alpacas have more than 22 natural coat colors, more than any other fiber producing species. To better understand the role of miRNAs in control of coat color we performed a comprehensive analysis of miRNA expression profiles in skin of white versus brown alpacas. RESULTS: Two small RNA libraries from white alpaca (WA) and brown alpaca (BA) skin were sequenced with the aid of Illumina sequencing technology. 272 and 267 conserved miRNAs were obtained from the WA and BA skin libraries, respectively. Of these conserved miRNAs, 35 and 13 were more abundant in WA and BA skin, respectively. The targets of these miRNAs were predicted and grouped based on Gene Ontology and KEGG pathway analysis. Many predicted target genes for these miRNAs are involved in the melanogenesis pathway controlling pigmentation. In addition to the conserved miRNAs, we also obtained 22 potentially novel miRNAs from the WA and BA skin libraries. CONCLUSION: This study represents the first comprehensive survey of miRNAs expressed in skin of animals of different coat colors by deep sequencing analysis. We discovered a collection of miRNAs that are differentially expressed in WA and BA skin. The results suggest important potential functions of miRNAs in coat color regulation. BioMed Central 2012-10-16 /pmc/articles/PMC3508611/ /pubmed/23067000 http://dx.doi.org/10.1186/1471-2164-13-555 Text en Copyright ©2012 Tian et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tian, Xue
Jiang, Junbing
Fan, Ruiwen
Wang, Haidong
Meng, Xiaolin
He, Xiaoyan
He, Junping
Li, Hongquan
Geng, Jianjun
Yu, Xiuju
Song, Yunfei
Zhang, Danli
Yao, Jianbo
Smith, George W
Dong, Changsheng
Identification and characterization of microRNAs in white and brown alpaca skin
title Identification and characterization of microRNAs in white and brown alpaca skin
title_full Identification and characterization of microRNAs in white and brown alpaca skin
title_fullStr Identification and characterization of microRNAs in white and brown alpaca skin
title_full_unstemmed Identification and characterization of microRNAs in white and brown alpaca skin
title_short Identification and characterization of microRNAs in white and brown alpaca skin
title_sort identification and characterization of micrornas in white and brown alpaca skin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3508611/
https://www.ncbi.nlm.nih.gov/pubmed/23067000
http://dx.doi.org/10.1186/1471-2164-13-555
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