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Isolation of inflammatory cells from rat brain tissue after stroke
The pathophysiology of sterile inflammation following focal ischemic stroke is complex and not fully understood, but there is growing evidence that it offers several therapeutic options beyond the hitherto existing treatment strategies. The identification and quantification of infiltrating inflammat...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3508842/ https://www.ncbi.nlm.nih.gov/pubmed/23031714 http://dx.doi.org/10.1186/2040-7378-4-20 |
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author | Möller, Karoline Stahl, Tobias Boltze, Johannes Wagner, Daniel-Christoph |
author_facet | Möller, Karoline Stahl, Tobias Boltze, Johannes Wagner, Daniel-Christoph |
author_sort | Möller, Karoline |
collection | PubMed |
description | The pathophysiology of sterile inflammation following focal ischemic stroke is complex and not fully understood, but there is growing evidence that it offers several therapeutic options beyond the hitherto existing treatment strategies. The identification and quantification of infiltrating inflammatory cells in animal models of stroke is crucial both for understanding post-stroke inflammation and for drug target identification. Multicolor flow cytometry plays an important role in determining subtypes and quantity of leukocytes that infiltrate the brain tissue after stroke. Until now, most investigations have been performed in mice, most likely due to a significantly broader spectrum of disposable antibodies and available knockout models. Here, we introduce a specific and reproducible method to isolate leukocytes from rat brain specimen in the context of brain ischemia to ultimately allow multi-dimensional flow cytometric characterization and further downstream methods such as cell-subtype sorting and molecular biological approaches. |
format | Online Article Text |
id | pubmed-3508842 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35088422012-11-29 Isolation of inflammatory cells from rat brain tissue after stroke Möller, Karoline Stahl, Tobias Boltze, Johannes Wagner, Daniel-Christoph Exp Transl Stroke Med Short Report The pathophysiology of sterile inflammation following focal ischemic stroke is complex and not fully understood, but there is growing evidence that it offers several therapeutic options beyond the hitherto existing treatment strategies. The identification and quantification of infiltrating inflammatory cells in animal models of stroke is crucial both for understanding post-stroke inflammation and for drug target identification. Multicolor flow cytometry plays an important role in determining subtypes and quantity of leukocytes that infiltrate the brain tissue after stroke. Until now, most investigations have been performed in mice, most likely due to a significantly broader spectrum of disposable antibodies and available knockout models. Here, we introduce a specific and reproducible method to isolate leukocytes from rat brain specimen in the context of brain ischemia to ultimately allow multi-dimensional flow cytometric characterization and further downstream methods such as cell-subtype sorting and molecular biological approaches. BioMed Central 2012-10-02 /pmc/articles/PMC3508842/ /pubmed/23031714 http://dx.doi.org/10.1186/2040-7378-4-20 Text en Copyright ©2012 Möller et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Möller, Karoline Stahl, Tobias Boltze, Johannes Wagner, Daniel-Christoph Isolation of inflammatory cells from rat brain tissue after stroke |
title | Isolation of inflammatory cells from rat brain tissue after stroke |
title_full | Isolation of inflammatory cells from rat brain tissue after stroke |
title_fullStr | Isolation of inflammatory cells from rat brain tissue after stroke |
title_full_unstemmed | Isolation of inflammatory cells from rat brain tissue after stroke |
title_short | Isolation of inflammatory cells from rat brain tissue after stroke |
title_sort | isolation of inflammatory cells from rat brain tissue after stroke |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3508842/ https://www.ncbi.nlm.nih.gov/pubmed/23031714 http://dx.doi.org/10.1186/2040-7378-4-20 |
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