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Donor Age-Related Biological Properties of Human Dental Pulp Stem Cells Change in Nanostructured Scaffolds

The aim of the present work is to study how biological properties, such as proliferation and commitment ability, of human adult dental pulp stem cells (DPSCs) relate to the age of the donor. Human dental pulps were extracted from molars of healthy adult subjects aged 16 to >66 years. DPSCs were i...

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Autores principales: Bressan, Eriberto, Ferroni, Letizia, Gardin, Chiara, Pinton, Paolo, Stellini, Edoardo, Botticelli, Daniele, Sivolella, Stefano, Zavan, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509126/
https://www.ncbi.nlm.nih.gov/pubmed/23209565
http://dx.doi.org/10.1371/journal.pone.0049146
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author Bressan, Eriberto
Ferroni, Letizia
Gardin, Chiara
Pinton, Paolo
Stellini, Edoardo
Botticelli, Daniele
Sivolella, Stefano
Zavan, Barbara
author_facet Bressan, Eriberto
Ferroni, Letizia
Gardin, Chiara
Pinton, Paolo
Stellini, Edoardo
Botticelli, Daniele
Sivolella, Stefano
Zavan, Barbara
author_sort Bressan, Eriberto
collection PubMed
description The aim of the present work is to study how biological properties, such as proliferation and commitment ability, of human adult dental pulp stem cells (DPSCs) relate to the age of the donor. Human dental pulps were extracted from molars of healthy adult subjects aged 16 to >66 years. DPSCs were isolated and cultured in the presence of osteogenic, neurogenic, or vasculogenic differentiation medium. Proliferation ability was evaluated by determining doubling time, and commitment ability was evaluated by gene expression and morphological analyses for tissue-specific markers. The results confirm a well-defined proliferative ability for each donor age group at an early in vitro passage (p2). DPSCs from younger donors (up to 35 years) maintain this ability in long-term cultures (p8). Stem cells of all age donor groups maintain their commitment ability during in vitro culture. In vivo tests on the critical size defect repair process confirmed that DPSCs of all donor ages are a potent tool for bone tissue regeneration when mixed with 3D nanostructured scaffolds.
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spelling pubmed-35091262012-12-03 Donor Age-Related Biological Properties of Human Dental Pulp Stem Cells Change in Nanostructured Scaffolds Bressan, Eriberto Ferroni, Letizia Gardin, Chiara Pinton, Paolo Stellini, Edoardo Botticelli, Daniele Sivolella, Stefano Zavan, Barbara PLoS One Research Article The aim of the present work is to study how biological properties, such as proliferation and commitment ability, of human adult dental pulp stem cells (DPSCs) relate to the age of the donor. Human dental pulps were extracted from molars of healthy adult subjects aged 16 to >66 years. DPSCs were isolated and cultured in the presence of osteogenic, neurogenic, or vasculogenic differentiation medium. Proliferation ability was evaluated by determining doubling time, and commitment ability was evaluated by gene expression and morphological analyses for tissue-specific markers. The results confirm a well-defined proliferative ability for each donor age group at an early in vitro passage (p2). DPSCs from younger donors (up to 35 years) maintain this ability in long-term cultures (p8). Stem cells of all age donor groups maintain their commitment ability during in vitro culture. In vivo tests on the critical size defect repair process confirmed that DPSCs of all donor ages are a potent tool for bone tissue regeneration when mixed with 3D nanostructured scaffolds. Public Library of Science 2012-11-28 /pmc/articles/PMC3509126/ /pubmed/23209565 http://dx.doi.org/10.1371/journal.pone.0049146 Text en © 2012 Bressan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bressan, Eriberto
Ferroni, Letizia
Gardin, Chiara
Pinton, Paolo
Stellini, Edoardo
Botticelli, Daniele
Sivolella, Stefano
Zavan, Barbara
Donor Age-Related Biological Properties of Human Dental Pulp Stem Cells Change in Nanostructured Scaffolds
title Donor Age-Related Biological Properties of Human Dental Pulp Stem Cells Change in Nanostructured Scaffolds
title_full Donor Age-Related Biological Properties of Human Dental Pulp Stem Cells Change in Nanostructured Scaffolds
title_fullStr Donor Age-Related Biological Properties of Human Dental Pulp Stem Cells Change in Nanostructured Scaffolds
title_full_unstemmed Donor Age-Related Biological Properties of Human Dental Pulp Stem Cells Change in Nanostructured Scaffolds
title_short Donor Age-Related Biological Properties of Human Dental Pulp Stem Cells Change in Nanostructured Scaffolds
title_sort donor age-related biological properties of human dental pulp stem cells change in nanostructured scaffolds
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509126/
https://www.ncbi.nlm.nih.gov/pubmed/23209565
http://dx.doi.org/10.1371/journal.pone.0049146
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