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In vivo assessment of cancerous tumors using boron doped diamond microelectrode

The in vitro and in vivo electrochemical detection of the reduced form of glutathione (L-γ-glutamyl-L-cysteinyl-glycine, GSH) using boron doped diamond (BDD) microelectrode for potential application in the assessment of cancerous tumors is presented. Accurate calibration curve for the determination...

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Autores principales: Fierro, Stéphane, Yoshikawa, Momoko, Nagano, Osamu, Yoshimi, Kenji, Saya, Hideyuki, Einaga, Yasuaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509351/
https://www.ncbi.nlm.nih.gov/pubmed/23198091
http://dx.doi.org/10.1038/srep00901
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author Fierro, Stéphane
Yoshikawa, Momoko
Nagano, Osamu
Yoshimi, Kenji
Saya, Hideyuki
Einaga, Yasuaki
author_facet Fierro, Stéphane
Yoshikawa, Momoko
Nagano, Osamu
Yoshimi, Kenji
Saya, Hideyuki
Einaga, Yasuaki
author_sort Fierro, Stéphane
collection PubMed
description The in vitro and in vivo electrochemical detection of the reduced form of glutathione (L-γ-glutamyl-L-cysteinyl-glycine, GSH) using boron doped diamond (BDD) microelectrode for potential application in the assessment of cancerous tumors is presented. Accurate calibration curve for the determination of GSH could be obtained by the in vitro electrochemical measurements. Additionally, it was shown that it was possible to separate the detection of GSH from the oxidized form of glutathione (GSSG) using chronoamperometry measurements. In vivo GSH detection measurements have been performed in human cancer cells inoculated in immunodeficient mice. These measurements have shown that the difference of GSH level between cancerous and normal tissues can be detected. Moreover, GSH detection measurements carried out before and after X-ray irradiation have proved that it is possible to assess in vivo the decrease in GSH concentration in the tumor after a specific treatment.
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spelling pubmed-35093512012-11-29 In vivo assessment of cancerous tumors using boron doped diamond microelectrode Fierro, Stéphane Yoshikawa, Momoko Nagano, Osamu Yoshimi, Kenji Saya, Hideyuki Einaga, Yasuaki Sci Rep Article The in vitro and in vivo electrochemical detection of the reduced form of glutathione (L-γ-glutamyl-L-cysteinyl-glycine, GSH) using boron doped diamond (BDD) microelectrode for potential application in the assessment of cancerous tumors is presented. Accurate calibration curve for the determination of GSH could be obtained by the in vitro electrochemical measurements. Additionally, it was shown that it was possible to separate the detection of GSH from the oxidized form of glutathione (GSSG) using chronoamperometry measurements. In vivo GSH detection measurements have been performed in human cancer cells inoculated in immunodeficient mice. These measurements have shown that the difference of GSH level between cancerous and normal tissues can be detected. Moreover, GSH detection measurements carried out before and after X-ray irradiation have proved that it is possible to assess in vivo the decrease in GSH concentration in the tumor after a specific treatment. Nature Publishing Group 2012-11-29 /pmc/articles/PMC3509351/ /pubmed/23198091 http://dx.doi.org/10.1038/srep00901 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
spellingShingle Article
Fierro, Stéphane
Yoshikawa, Momoko
Nagano, Osamu
Yoshimi, Kenji
Saya, Hideyuki
Einaga, Yasuaki
In vivo assessment of cancerous tumors using boron doped diamond microelectrode
title In vivo assessment of cancerous tumors using boron doped diamond microelectrode
title_full In vivo assessment of cancerous tumors using boron doped diamond microelectrode
title_fullStr In vivo assessment of cancerous tumors using boron doped diamond microelectrode
title_full_unstemmed In vivo assessment of cancerous tumors using boron doped diamond microelectrode
title_short In vivo assessment of cancerous tumors using boron doped diamond microelectrode
title_sort in vivo assessment of cancerous tumors using boron doped diamond microelectrode
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509351/
https://www.ncbi.nlm.nih.gov/pubmed/23198091
http://dx.doi.org/10.1038/srep00901
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