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Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin
In the present paper, the effect of β-cyclodextrin (β-CD) inclusion complexation on the solubility and enzymatic hydrolysis of naringin was investigated. The inclusion complex of naringin/β-CD at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scannin...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509578/ https://www.ncbi.nlm.nih.gov/pubmed/23203062 http://dx.doi.org/10.3390/ijms131114251 |
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author | Cui, Li Zhang, Zhen-Hai Sun, E Jia, Xiao-Bin |
author_facet | Cui, Li Zhang, Zhen-Hai Sun, E Jia, Xiao-Bin |
author_sort | Cui, Li |
collection | PubMed |
description | In the present paper, the effect of β-cyclodextrin (β-CD) inclusion complexation on the solubility and enzymatic hydrolysis of naringin was investigated. The inclusion complex of naringin/β-CD at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of naringin complexes in water at 37 ± 0.1 °C was 15 times greater than that of free naringin. Snailase-involved hydrolysis conditions were tested for the bioconversion of naringin into naringenin using the univariate experimental design. Naringin can be transformed into naringenin by snailase-involved hydrolysis. The optimum conditions for enzymatic hydrolysis were determined as follows: pH 5.0, temperature 37 °C, ratio of snailase/substrate 0.8, substrate concentration 20 mg·mL(−1), and reaction time 12 h. Under the optimum conditions, the transforming rate of naringenin from naringin for inclusion complexes and free naringin was 98.7% and 56.2% respectively, suggesting that β-CD complexation can improve the aqueous solubility and consequently the enzymatic hydrolysis rate of naringin. |
format | Online Article Text |
id | pubmed-3509578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-35095782013-01-09 Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin Cui, Li Zhang, Zhen-Hai Sun, E Jia, Xiao-Bin Int J Mol Sci Article In the present paper, the effect of β-cyclodextrin (β-CD) inclusion complexation on the solubility and enzymatic hydrolysis of naringin was investigated. The inclusion complex of naringin/β-CD at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of naringin complexes in water at 37 ± 0.1 °C was 15 times greater than that of free naringin. Snailase-involved hydrolysis conditions were tested for the bioconversion of naringin into naringenin using the univariate experimental design. Naringin can be transformed into naringenin by snailase-involved hydrolysis. The optimum conditions for enzymatic hydrolysis were determined as follows: pH 5.0, temperature 37 °C, ratio of snailase/substrate 0.8, substrate concentration 20 mg·mL(−1), and reaction time 12 h. Under the optimum conditions, the transforming rate of naringenin from naringin for inclusion complexes and free naringin was 98.7% and 56.2% respectively, suggesting that β-CD complexation can improve the aqueous solubility and consequently the enzymatic hydrolysis rate of naringin. Molecular Diversity Preservation International (MDPI) 2012-11-05 /pmc/articles/PMC3509578/ /pubmed/23203062 http://dx.doi.org/10.3390/ijms131114251 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0). |
spellingShingle | Article Cui, Li Zhang, Zhen-Hai Sun, E Jia, Xiao-Bin Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin |
title | Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin |
title_full | Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin |
title_fullStr | Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin |
title_full_unstemmed | Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin |
title_short | Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin |
title_sort | effect of β-cyclodextrin complexation on solubility and enzymatic conversion of naringin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509578/ https://www.ncbi.nlm.nih.gov/pubmed/23203062 http://dx.doi.org/10.3390/ijms131114251 |
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