Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library

Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The o...

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Autores principales: Mezghani-Khemakhem, Maha, Bouktila, Dhia, Casse, Nathalie, Maaroufi, Houcine, Makni, Mohamed, Makni, Hanem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509590/
https://www.ncbi.nlm.nih.gov/pubmed/23203074
http://dx.doi.org/10.3390/ijms131114446
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author Mezghani-Khemakhem, Maha
Bouktila, Dhia
Casse, Nathalie
Maaroufi, Houcine
Makni, Mohamed
Makni, Hanem
author_facet Mezghani-Khemakhem, Maha
Bouktila, Dhia
Casse, Nathalie
Maaroufi, Houcine
Makni, Mohamed
Makni, Hanem
author_sort Mezghani-Khemakhem, Maha
collection PubMed
description Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The observed heterozygosity varied between 0.070 and 0.877. Based on the number of alleles detected and the associated levels of heterozygosity, we believe that these loci will prove useful for population genetic studies on M. hordei.
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spelling pubmed-35095902013-01-09 Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library Mezghani-Khemakhem, Maha Bouktila, Dhia Casse, Nathalie Maaroufi, Houcine Makni, Mohamed Makni, Hanem Int J Mol Sci Article Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The observed heterozygosity varied between 0.070 and 0.877. Based on the number of alleles detected and the associated levels of heterozygosity, we believe that these loci will prove useful for population genetic studies on M. hordei. Molecular Diversity Preservation International (MDPI) 2012-11-08 /pmc/articles/PMC3509590/ /pubmed/23203074 http://dx.doi.org/10.3390/ijms131114446 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0).
spellingShingle Article
Mezghani-Khemakhem, Maha
Bouktila, Dhia
Casse, Nathalie
Maaroufi, Houcine
Makni, Mohamed
Makni, Hanem
Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library
title Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library
title_full Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library
title_fullStr Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library
title_full_unstemmed Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library
title_short Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library
title_sort development of new polymorphic microsatellite loci for the barley stem gall midge, mayetiola hordei (diptera: cecidomyiidae) from an enriched library
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509590/
https://www.ncbi.nlm.nih.gov/pubmed/23203074
http://dx.doi.org/10.3390/ijms131114446
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