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A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS)
The oral adsorbent AST-120 is composed of spherical carbon particles and has an adsorption ability for certain small-molecular-weight compounds that accumulate in patients with chronic kidney disease (CKD). So far, very few compounds are known to be adsorbed by AST-120 in vivo. To examine the effect...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509710/ https://www.ncbi.nlm.nih.gov/pubmed/23202318 http://dx.doi.org/10.3390/toxins4111309 |
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author | Akiyama, Yasutoshi Takeuchi, Yoichi Kikuchi, Koichi Mishima, Eikan Yamamoto, Yasuaki Suzuki, Chitose Toyohara, Takafumi Suzuki, Takehiro Hozawa, Atsushi Ito, Sadayoshi Soga, Tomoyoshi Abe, Takaaki |
author_facet | Akiyama, Yasutoshi Takeuchi, Yoichi Kikuchi, Koichi Mishima, Eikan Yamamoto, Yasuaki Suzuki, Chitose Toyohara, Takafumi Suzuki, Takehiro Hozawa, Atsushi Ito, Sadayoshi Soga, Tomoyoshi Abe, Takaaki |
author_sort | Akiyama, Yasutoshi |
collection | PubMed |
description | The oral adsorbent AST-120 is composed of spherical carbon particles and has an adsorption ability for certain small-molecular-weight compounds that accumulate in patients with chronic kidney disease (CKD). So far, very few compounds are known to be adsorbed by AST-120 in vivo. To examine the effect of AST-120 in vivo, we comprehensively evaluated the plasma concentrations of 146 compounds (61 anions and 85 cations) in CKD model rats, with or without four weeks of treatment with AST-120. By capillary electrophoresis with mass spectrometry, we identified 6 anions and 17 cations that were significantly decreased by AST-120 treatment. In contrast, we also identified 2 cations that were significantly increased by AST-120. Among them, 4 anions, apart from indoxyl sulfate and hippurate, and 19 cations were newly identified in this study. The plasma levels of N-acetyl-neuraminate, 4-pyridoxate, 4-oxopentanoate, glycine, γ-guanidinobutyrate, N-γ-ethylglutamine, allantoin, cytosine, 5-methylcytosine and imidazole-4-acetate were significantly increased in the CKD model compared with the sham-operated group, and were significantly decreased by AST-120 treatment. Therefore, these 10 compounds could be added as uremic compounds that indicate the effect of AST-120 treatment. This study provides useful information not only for identifying the indicators of AST-120, but also for clarifying changes in the metabolic profile by AST-120 treatment in the clinical setting. |
format | Online Article Text |
id | pubmed-3509710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-35097102012-12-10 A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) Akiyama, Yasutoshi Takeuchi, Yoichi Kikuchi, Koichi Mishima, Eikan Yamamoto, Yasuaki Suzuki, Chitose Toyohara, Takafumi Suzuki, Takehiro Hozawa, Atsushi Ito, Sadayoshi Soga, Tomoyoshi Abe, Takaaki Toxins (Basel) Article The oral adsorbent AST-120 is composed of spherical carbon particles and has an adsorption ability for certain small-molecular-weight compounds that accumulate in patients with chronic kidney disease (CKD). So far, very few compounds are known to be adsorbed by AST-120 in vivo. To examine the effect of AST-120 in vivo, we comprehensively evaluated the plasma concentrations of 146 compounds (61 anions and 85 cations) in CKD model rats, with or without four weeks of treatment with AST-120. By capillary electrophoresis with mass spectrometry, we identified 6 anions and 17 cations that were significantly decreased by AST-120 treatment. In contrast, we also identified 2 cations that were significantly increased by AST-120. Among them, 4 anions, apart from indoxyl sulfate and hippurate, and 19 cations were newly identified in this study. The plasma levels of N-acetyl-neuraminate, 4-pyridoxate, 4-oxopentanoate, glycine, γ-guanidinobutyrate, N-γ-ethylglutamine, allantoin, cytosine, 5-methylcytosine and imidazole-4-acetate were significantly increased in the CKD model compared with the sham-operated group, and were significantly decreased by AST-120 treatment. Therefore, these 10 compounds could be added as uremic compounds that indicate the effect of AST-120 treatment. This study provides useful information not only for identifying the indicators of AST-120, but also for clarifying changes in the metabolic profile by AST-120 treatment in the clinical setting. MDPI 2012-11-14 /pmc/articles/PMC3509710/ /pubmed/23202318 http://dx.doi.org/10.3390/toxins4111309 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Akiyama, Yasutoshi Takeuchi, Yoichi Kikuchi, Koichi Mishima, Eikan Yamamoto, Yasuaki Suzuki, Chitose Toyohara, Takafumi Suzuki, Takehiro Hozawa, Atsushi Ito, Sadayoshi Soga, Tomoyoshi Abe, Takaaki A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) |
title | A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) |
title_full | A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) |
title_fullStr | A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) |
title_full_unstemmed | A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) |
title_short | A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS) |
title_sort | metabolomic approach to clarifying the effect of ast-120 on 5/6 nephrectomized rats by capillary electrophoresis with mass spectrometry (ce-ms) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509710/ https://www.ncbi.nlm.nih.gov/pubmed/23202318 http://dx.doi.org/10.3390/toxins4111309 |
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