Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex

Chromatin boundaries subdivide eukaryotic chromosomes into functionally autonomous domains of genetic activity. This subdivision insulates genes and/or regulatory elements within a domain from promiscuous interactions with nearby domains. While it was previously assumed that the chromosomal domain l...

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Detalles Bibliográficos
Autores principales: Aoki, Tsutomu, Sarkeshik, Ali, Yates, John, Schedl, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2012
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510454/
https://www.ncbi.nlm.nih.gov/pubmed/23240086
http://dx.doi.org/10.7554/eLife.00171
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author Aoki, Tsutomu
Sarkeshik, Ali
Yates, John
Schedl, Paul
author_facet Aoki, Tsutomu
Sarkeshik, Ali
Yates, John
Schedl, Paul
author_sort Aoki, Tsutomu
collection PubMed
description Chromatin boundaries subdivide eukaryotic chromosomes into functionally autonomous domains of genetic activity. This subdivision insulates genes and/or regulatory elements within a domain from promiscuous interactions with nearby domains. While it was previously assumed that the chromosomal domain landscape is fixed, there is now growing evidence that the landscape may be subject to tissue and stage specific regulation. Here we report the isolation and characterization of a novel developmentally restricted boundary factor, Elba. We show that Elba is an unusual hetero-tripartite protein complex that requires all three proteins for DNA binding and insulator activity. DOI: http://dx.doi.org/10.7554/eLife.00171.001
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spelling pubmed-35104542012-12-14 Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex Aoki, Tsutomu Sarkeshik, Ali Yates, John Schedl, Paul eLife Biochemistry Chromatin boundaries subdivide eukaryotic chromosomes into functionally autonomous domains of genetic activity. This subdivision insulates genes and/or regulatory elements within a domain from promiscuous interactions with nearby domains. While it was previously assumed that the chromosomal domain landscape is fixed, there is now growing evidence that the landscape may be subject to tissue and stage specific regulation. Here we report the isolation and characterization of a novel developmentally restricted boundary factor, Elba. We show that Elba is an unusual hetero-tripartite protein complex that requires all three proteins for DNA binding and insulator activity. DOI: http://dx.doi.org/10.7554/eLife.00171.001 eLife Sciences Publications, Ltd 2012-12-13 /pmc/articles/PMC3510454/ /pubmed/23240086 http://dx.doi.org/10.7554/eLife.00171 Text en Copyright © 2012, Aoki et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry
Aoki, Tsutomu
Sarkeshik, Ali
Yates, John
Schedl, Paul
Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex
title Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex
title_full Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex
title_fullStr Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex
title_full_unstemmed Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex
title_short Elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite DNA binding complex
title_sort elba, a novel developmentally regulated chromatin boundary factor is a hetero-tripartite dna binding complex
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510454/
https://www.ncbi.nlm.nih.gov/pubmed/23240086
http://dx.doi.org/10.7554/eLife.00171
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AT schedlpaul elbaanoveldevelopmentallyregulatedchromatinboundaryfactorisaheterotripartitednabindingcomplex

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