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Protective effects of natsumikan (Citrus natsudaidai) extract on acetaminophen-induced lethal hepatotoxicity in mice

BACKGROUND: Like other citrus fruits, natsumikan (Citrus natsudaidai) contains several antioxidative nutrients which occur in higher concentrations in the peel than in the pulp. A high dose of acetaminophen (APAP) generates highly reactive intermediates and causes fatal liver injury. In this study,...

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Autores principales: Yamaura, Katsunori, Nakayama, Noriyuki, Shimada, Maki, Ueno, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510878/
https://www.ncbi.nlm.nih.gov/pubmed/23225969
http://dx.doi.org/10.4103/0974-8490.102274
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author Yamaura, Katsunori
Nakayama, Noriyuki
Shimada, Maki
Ueno, Koichi
author_facet Yamaura, Katsunori
Nakayama, Noriyuki
Shimada, Maki
Ueno, Koichi
author_sort Yamaura, Katsunori
collection PubMed
description BACKGROUND: Like other citrus fruits, natsumikan (Citrus natsudaidai) contains several antioxidative nutrients which occur in higher concentrations in the peel than in the pulp. A high dose of acetaminophen (APAP) generates highly reactive intermediates and causes fatal liver injury. In this study, we examined whether an extract from immature natsumikan peel prevents lethal hepatotoxicity induced by a lethal dose of APAP in mice. MATERIALS AND METHODS: Male ICR mice were treated orally with natsumikan extract (300 and 1,000 mg/kg) 2, 26, and 50 h before single oral APAP (300 mg/kg) administration. Mice were fasted for 18 h before APAP treatment, but given tap water ad libitum. Survival was assessed for 24 h after APAP treatment. RESULTS: Following administration of 300 mg/kg APAP, all mice died within 6 h. However, pretreatment with natsumikan extract (300 and 1,000 mg/kg) or silymarin (300 and 1,000 mg/kg) increased the survival rate to 16.7%, 33.3%, 16.7%, and 50%, respectively, at 24 h. CONCLUSION: The results suggest that natsumikan has a protective effect on APAP-induced lethal hepatotoxicity.
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spelling pubmed-35108782012-12-05 Protective effects of natsumikan (Citrus natsudaidai) extract on acetaminophen-induced lethal hepatotoxicity in mice Yamaura, Katsunori Nakayama, Noriyuki Shimada, Maki Ueno, Koichi Pharmacognosy Res Short Communication BACKGROUND: Like other citrus fruits, natsumikan (Citrus natsudaidai) contains several antioxidative nutrients which occur in higher concentrations in the peel than in the pulp. A high dose of acetaminophen (APAP) generates highly reactive intermediates and causes fatal liver injury. In this study, we examined whether an extract from immature natsumikan peel prevents lethal hepatotoxicity induced by a lethal dose of APAP in mice. MATERIALS AND METHODS: Male ICR mice were treated orally with natsumikan extract (300 and 1,000 mg/kg) 2, 26, and 50 h before single oral APAP (300 mg/kg) administration. Mice were fasted for 18 h before APAP treatment, but given tap water ad libitum. Survival was assessed for 24 h after APAP treatment. RESULTS: Following administration of 300 mg/kg APAP, all mice died within 6 h. However, pretreatment with natsumikan extract (300 and 1,000 mg/kg) or silymarin (300 and 1,000 mg/kg) increased the survival rate to 16.7%, 33.3%, 16.7%, and 50%, respectively, at 24 h. CONCLUSION: The results suggest that natsumikan has a protective effect on APAP-induced lethal hepatotoxicity. Medknow Publications & Media Pvt Ltd 2012 /pmc/articles/PMC3510878/ /pubmed/23225969 http://dx.doi.org/10.4103/0974-8490.102274 Text en Copyright: © Pharmacognosy Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Yamaura, Katsunori
Nakayama, Noriyuki
Shimada, Maki
Ueno, Koichi
Protective effects of natsumikan (Citrus natsudaidai) extract on acetaminophen-induced lethal hepatotoxicity in mice
title Protective effects of natsumikan (Citrus natsudaidai) extract on acetaminophen-induced lethal hepatotoxicity in mice
title_full Protective effects of natsumikan (Citrus natsudaidai) extract on acetaminophen-induced lethal hepatotoxicity in mice
title_fullStr Protective effects of natsumikan (Citrus natsudaidai) extract on acetaminophen-induced lethal hepatotoxicity in mice
title_full_unstemmed Protective effects of natsumikan (Citrus natsudaidai) extract on acetaminophen-induced lethal hepatotoxicity in mice
title_short Protective effects of natsumikan (Citrus natsudaidai) extract on acetaminophen-induced lethal hepatotoxicity in mice
title_sort protective effects of natsumikan (citrus natsudaidai) extract on acetaminophen-induced lethal hepatotoxicity in mice
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510878/
https://www.ncbi.nlm.nih.gov/pubmed/23225969
http://dx.doi.org/10.4103/0974-8490.102274
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