Optogenetic Control of Transcription in Zebrafish

Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription syste...

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Detalles Bibliográficos
Autores principales: Liu, Hongtao, Gomez, Gustavo, Lin, Sophia, Lin, Shuo, Lin, Chentao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3511356/
https://www.ncbi.nlm.nih.gov/pubmed/23226369
http://dx.doi.org/10.1371/journal.pone.0050738
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author Liu, Hongtao
Gomez, Gustavo
Lin, Sophia
Lin, Shuo
Lin, Chentao
author_facet Liu, Hongtao
Gomez, Gustavo
Lin, Sophia
Lin, Shuo
Lin, Chentao
author_sort Liu, Hongtao
collection PubMed
description Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2) and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1). We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish.
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spelling pubmed-35113562012-12-05 Optogenetic Control of Transcription in Zebrafish Liu, Hongtao Gomez, Gustavo Lin, Sophia Lin, Shuo Lin, Chentao PLoS One Research Article Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2) and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1). We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish. Public Library of Science 2012-11-30 /pmc/articles/PMC3511356/ /pubmed/23226369 http://dx.doi.org/10.1371/journal.pone.0050738 Text en © 2012 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Hongtao
Gomez, Gustavo
Lin, Sophia
Lin, Shuo
Lin, Chentao
Optogenetic Control of Transcription in Zebrafish
title Optogenetic Control of Transcription in Zebrafish
title_full Optogenetic Control of Transcription in Zebrafish
title_fullStr Optogenetic Control of Transcription in Zebrafish
title_full_unstemmed Optogenetic Control of Transcription in Zebrafish
title_short Optogenetic Control of Transcription in Zebrafish
title_sort optogenetic control of transcription in zebrafish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3511356/
https://www.ncbi.nlm.nih.gov/pubmed/23226369
http://dx.doi.org/10.1371/journal.pone.0050738
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