Cargando…
Downregulation of GluA2 AMPA Receptor Subunits Reduces the Dendritic Arborization of Developing Spinal Motoneurons
AMPA receptors lacking the GluA2 subunit allow a significant influx of Ca(2+) ions. Although Ca(2+)-permeable AMPA receptors are a familiar feature at early stages of development, the functional significance of these receptors during the maturation of the nervous system remains to be established. Ch...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3511505/ https://www.ncbi.nlm.nih.gov/pubmed/23226228 http://dx.doi.org/10.1371/journal.pone.0049879 |
_version_ | 1782251624379449344 |
---|---|
author | Yoon, Yone J. White, Sheryl L. Ni, Xianglian Gokin, Alexander P. Martin-Caraballo, Miguel |
author_facet | Yoon, Yone J. White, Sheryl L. Ni, Xianglian Gokin, Alexander P. Martin-Caraballo, Miguel |
author_sort | Yoon, Yone J. |
collection | PubMed |
description | AMPA receptors lacking the GluA2 subunit allow a significant influx of Ca(2+) ions. Although Ca(2+)-permeable AMPA receptors are a familiar feature at early stages of development, the functional significance of these receptors during the maturation of the nervous system remains to be established. Chicken lumbar motoneurons express Ca(2+)-permeable AMPA receptors at E6 but the Ca(2+) permeability of AMPA receptors decreases ∼3-fold by E11. Considering that activity-dependent changes in intracellular Ca(2+) regulates dendritic outgrowth, in this study we investigated whether downregulation of GluA2 expression during a critical period of development alters the dendritic arborization of spinal motoneurons in ovo. We use an avian replication-competent retroviral vector RCASBP (B) carrying the marker red fluorescent protein (RFP) and a GluA2 RNAi construct to downregulate GluA2 expression. Chicken embryos were infected at E2 with one of the following constructs: RCASBP(B)-RFP, RCASBP(B)-RFP-scrambled RNAi, or RCASBP(B)-RFP-GluA2 RNAi. Infection of chicken embryos at E2 resulted in widespread expression of RFP throughout the spinal cord with ≥60% of Islet1/2-positive motoneurons infected, resulting in a significant reduction in GluA2 protein expression. Downregulation of GluA2 expression had no effect on the dendritic arborization of E6 motoneurons. However, downregulation of GluA2 expression caused a significant reduction in the dendritic arborization of E11 motoneurons. Neither motoneuron survival nor maturation of network activity was affected by changes in GluA2 expression. These findings demonstrate that increased GluA2 expression and changes in the Ca(2+) permeability of AMPA receptors regulate the dendritic arborization of spinal cord motoneurons during a critical period of development. |
format | Online Article Text |
id | pubmed-3511505 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35115052012-12-05 Downregulation of GluA2 AMPA Receptor Subunits Reduces the Dendritic Arborization of Developing Spinal Motoneurons Yoon, Yone J. White, Sheryl L. Ni, Xianglian Gokin, Alexander P. Martin-Caraballo, Miguel PLoS One Research Article AMPA receptors lacking the GluA2 subunit allow a significant influx of Ca(2+) ions. Although Ca(2+)-permeable AMPA receptors are a familiar feature at early stages of development, the functional significance of these receptors during the maturation of the nervous system remains to be established. Chicken lumbar motoneurons express Ca(2+)-permeable AMPA receptors at E6 but the Ca(2+) permeability of AMPA receptors decreases ∼3-fold by E11. Considering that activity-dependent changes in intracellular Ca(2+) regulates dendritic outgrowth, in this study we investigated whether downregulation of GluA2 expression during a critical period of development alters the dendritic arborization of spinal motoneurons in ovo. We use an avian replication-competent retroviral vector RCASBP (B) carrying the marker red fluorescent protein (RFP) and a GluA2 RNAi construct to downregulate GluA2 expression. Chicken embryos were infected at E2 with one of the following constructs: RCASBP(B)-RFP, RCASBP(B)-RFP-scrambled RNAi, or RCASBP(B)-RFP-GluA2 RNAi. Infection of chicken embryos at E2 resulted in widespread expression of RFP throughout the spinal cord with ≥60% of Islet1/2-positive motoneurons infected, resulting in a significant reduction in GluA2 protein expression. Downregulation of GluA2 expression had no effect on the dendritic arborization of E6 motoneurons. However, downregulation of GluA2 expression caused a significant reduction in the dendritic arborization of E11 motoneurons. Neither motoneuron survival nor maturation of network activity was affected by changes in GluA2 expression. These findings demonstrate that increased GluA2 expression and changes in the Ca(2+) permeability of AMPA receptors regulate the dendritic arborization of spinal cord motoneurons during a critical period of development. Public Library of Science 2012-11-30 /pmc/articles/PMC3511505/ /pubmed/23226228 http://dx.doi.org/10.1371/journal.pone.0049879 Text en © 2012 Yoon et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Yoon, Yone J. White, Sheryl L. Ni, Xianglian Gokin, Alexander P. Martin-Caraballo, Miguel Downregulation of GluA2 AMPA Receptor Subunits Reduces the Dendritic Arborization of Developing Spinal Motoneurons |
title | Downregulation of GluA2 AMPA Receptor Subunits Reduces the Dendritic Arborization of Developing Spinal Motoneurons |
title_full | Downregulation of GluA2 AMPA Receptor Subunits Reduces the Dendritic Arborization of Developing Spinal Motoneurons |
title_fullStr | Downregulation of GluA2 AMPA Receptor Subunits Reduces the Dendritic Arborization of Developing Spinal Motoneurons |
title_full_unstemmed | Downregulation of GluA2 AMPA Receptor Subunits Reduces the Dendritic Arborization of Developing Spinal Motoneurons |
title_short | Downregulation of GluA2 AMPA Receptor Subunits Reduces the Dendritic Arborization of Developing Spinal Motoneurons |
title_sort | downregulation of glua2 ampa receptor subunits reduces the dendritic arborization of developing spinal motoneurons |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3511505/ https://www.ncbi.nlm.nih.gov/pubmed/23226228 http://dx.doi.org/10.1371/journal.pone.0049879 |
work_keys_str_mv | AT yoonyonej downregulationofglua2ampareceptorsubunitsreducesthedendriticarborizationofdevelopingspinalmotoneurons AT whitesheryll downregulationofglua2ampareceptorsubunitsreducesthedendriticarborizationofdevelopingspinalmotoneurons AT nixianglian downregulationofglua2ampareceptorsubunitsreducesthedendriticarborizationofdevelopingspinalmotoneurons AT gokinalexanderp downregulationofglua2ampareceptorsubunitsreducesthedendriticarborizationofdevelopingspinalmotoneurons AT martincaraballomiguel downregulationofglua2ampareceptorsubunitsreducesthedendriticarborizationofdevelopingspinalmotoneurons |