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9 A Proteomic Style Approach to Characterize a Grass Mix Product Reveals Potential Immunotherapeutic Benefit
BACKGROUND: Grass allergy immunotherapies often consist of a mix of different grass extracts each containing several proteins of different physiochemical properties; however the subtle contributions of each protein are difficult to elucidate. This study aimed to identify and characterise the group 1...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
World Allergy Organization Journal
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3512597/ http://dx.doi.org/10.1097/01.WOX.0000411754.75035.ed |
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author | Skinner, Murray Bullimore, Alan Swanc, Nicola Alawode, Wemimo |
author_facet | Skinner, Murray Bullimore, Alan Swanc, Nicola Alawode, Wemimo |
author_sort | Skinner, Murray |
collection | PubMed |
description | BACKGROUND: Grass allergy immunotherapies often consist of a mix of different grass extracts each containing several proteins of different physiochemical properties; however the subtle contributions of each protein are difficult to elucidate. This study aimed to identify and characterise the group 1 and 5 allergens in a 13 grass extract and to standardise the extraction method. METHODS: The grass pollens were extracted in isolation and pooled and also in combination and analysed using a variety of techniques including enzyme-linked immunosorbent assay (ELISA), liquid chromatography-mass spectrometry (LC-MS) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: Gold-staining and IgE immunoblotting revealed a high degree of homology of protein bands between the 13 species and the presence of a densely stained doublet at 25 to 35 kD along with protein bands at approximately 12.5, 17 and 50 kD. The doublet from each grass species demonstrated a high level of group 1 and 5 interspecies homology. However, there were a number of bands unique to specific grasses consistent with evolutionary change and indicative that a grass mix immunotherapeutic could be considered broad spectrum. CONCLUSIONS: SDS-PAGE and IgE immunoblotting showed all 13 grasses share a high degree of homology particularly in terms of group 1 and 5 allergens. IgE and IgG ELISA potencies were shown to be independent of extraction method. |
format | Online Article Text |
id | pubmed-3512597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | World Allergy Organization Journal |
record_format | MEDLINE/PubMed |
spelling | pubmed-35125972012-12-21 9 A Proteomic Style Approach to Characterize a Grass Mix Product Reveals Potential Immunotherapeutic Benefit Skinner, Murray Bullimore, Alan Swanc, Nicola Alawode, Wemimo World Allergy Organ J Abstracts of the XXII World Allergy Congress BACKGROUND: Grass allergy immunotherapies often consist of a mix of different grass extracts each containing several proteins of different physiochemical properties; however the subtle contributions of each protein are difficult to elucidate. This study aimed to identify and characterise the group 1 and 5 allergens in a 13 grass extract and to standardise the extraction method. METHODS: The grass pollens were extracted in isolation and pooled and also in combination and analysed using a variety of techniques including enzyme-linked immunosorbent assay (ELISA), liquid chromatography-mass spectrometry (LC-MS) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: Gold-staining and IgE immunoblotting revealed a high degree of homology of protein bands between the 13 species and the presence of a densely stained doublet at 25 to 35 kD along with protein bands at approximately 12.5, 17 and 50 kD. The doublet from each grass species demonstrated a high level of group 1 and 5 interspecies homology. However, there were a number of bands unique to specific grasses consistent with evolutionary change and indicative that a grass mix immunotherapeutic could be considered broad spectrum. CONCLUSIONS: SDS-PAGE and IgE immunoblotting showed all 13 grasses share a high degree of homology particularly in terms of group 1 and 5 allergens. IgE and IgG ELISA potencies were shown to be independent of extraction method. World Allergy Organization Journal 2012-02-17 /pmc/articles/PMC3512597/ http://dx.doi.org/10.1097/01.WOX.0000411754.75035.ed Text en Copyright © 2012 by World Allergy Organization |
spellingShingle | Abstracts of the XXII World Allergy Congress Skinner, Murray Bullimore, Alan Swanc, Nicola Alawode, Wemimo 9 A Proteomic Style Approach to Characterize a Grass Mix Product Reveals Potential Immunotherapeutic Benefit |
title | 9 A Proteomic Style Approach to Characterize a Grass Mix Product Reveals Potential Immunotherapeutic Benefit |
title_full | 9 A Proteomic Style Approach to Characterize a Grass Mix Product Reveals Potential Immunotherapeutic Benefit |
title_fullStr | 9 A Proteomic Style Approach to Characterize a Grass Mix Product Reveals Potential Immunotherapeutic Benefit |
title_full_unstemmed | 9 A Proteomic Style Approach to Characterize a Grass Mix Product Reveals Potential Immunotherapeutic Benefit |
title_short | 9 A Proteomic Style Approach to Characterize a Grass Mix Product Reveals Potential Immunotherapeutic Benefit |
title_sort | 9 a proteomic style approach to characterize a grass mix product reveals potential immunotherapeutic benefit |
topic | Abstracts of the XXII World Allergy Congress |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3512597/ http://dx.doi.org/10.1097/01.WOX.0000411754.75035.ed |
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