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89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells?
BACKGROUND: The exact mechanism of systemic hypersensitivity to venom is not exactly understood. It is suggested T cells with regulatory potential can downregulate other T cell subsets and effector cells, ex. mast cell or basophils. We focused on relationship of specific basophil reactivity in relat...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
World Allergy Organization Journal
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3512905/ http://dx.doi.org/10.1097/01.WOX.0000411834.23118.10 |
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author | Kucera, Petr Hulikova, Katarina Cvackova, Milada Planska, Daniela Riegerova, Kamila |
author_facet | Kucera, Petr Hulikova, Katarina Cvackova, Milada Planska, Daniela Riegerova, Kamila |
author_sort | Kucera, Petr |
collection | PubMed |
description | BACKGROUND: The exact mechanism of systemic hypersensitivity to venom is not exactly understood. It is suggested T cells with regulatory potential can downregulate other T cell subsets and effector cells, ex. mast cell or basophils. We focused on relationship of specific basophil reactivity in relationship to proportion of regulatory T cells. METHODS: Forty-five patients with history of systemic symptoms of allergy to Hymenoptera venom were included. Basophil reactivity before the treatment and after one year of allergen immunotherapy (AIT) was measured by CD63 expression, CD203c marker used for basophil identification. Cells were stimulated by aqueous solution of allergen in concentration range 0.01 to 1 μg/mL. T regulatory cells were identified as CD4 positive, CD25 bright and CD127 negative at the same interval of treatment. Monoclonal antibodies conjugated with fluorochromes were used, measured by FACSCalibur. Paired t test and correlation analysis used for statistical evaluation. RESULTS: Median Treg proportion before therapy was 2.160, after IT 1.960, basophil specific response (proportion of CD63 positive cells) at the same interval were 3.65 and 4.11 at 0.01 μg/mL, 13.1 and 16.1 for 0.1 μg/mL and 33.85 and 40.8 for 1 μg/mL. All differences were not statistically significant. Differences of basophil activation were not significantly related to proportion of T regulatory cells. CONCLUSIONS: In our group of patients with HV allergy, treated by AIT, we did not found any relationship between basophil specific activation during allergen immunotherapy and proportion of T regulatory cells. |
format | Online Article Text |
id | pubmed-3512905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | World Allergy Organization Journal |
record_format | MEDLINE/PubMed |
spelling | pubmed-35129052012-12-21 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells? Kucera, Petr Hulikova, Katarina Cvackova, Milada Planska, Daniela Riegerova, Kamila World Allergy Organ J Abstracts of the XXII World Allergy Congress BACKGROUND: The exact mechanism of systemic hypersensitivity to venom is not exactly understood. It is suggested T cells with regulatory potential can downregulate other T cell subsets and effector cells, ex. mast cell or basophils. We focused on relationship of specific basophil reactivity in relationship to proportion of regulatory T cells. METHODS: Forty-five patients with history of systemic symptoms of allergy to Hymenoptera venom were included. Basophil reactivity before the treatment and after one year of allergen immunotherapy (AIT) was measured by CD63 expression, CD203c marker used for basophil identification. Cells were stimulated by aqueous solution of allergen in concentration range 0.01 to 1 μg/mL. T regulatory cells were identified as CD4 positive, CD25 bright and CD127 negative at the same interval of treatment. Monoclonal antibodies conjugated with fluorochromes were used, measured by FACSCalibur. Paired t test and correlation analysis used for statistical evaluation. RESULTS: Median Treg proportion before therapy was 2.160, after IT 1.960, basophil specific response (proportion of CD63 positive cells) at the same interval were 3.65 and 4.11 at 0.01 μg/mL, 13.1 and 16.1 for 0.1 μg/mL and 33.85 and 40.8 for 1 μg/mL. All differences were not statistically significant. Differences of basophil activation were not significantly related to proportion of T regulatory cells. CONCLUSIONS: In our group of patients with HV allergy, treated by AIT, we did not found any relationship between basophil specific activation during allergen immunotherapy and proportion of T regulatory cells. World Allergy Organization Journal 2012-02-17 /pmc/articles/PMC3512905/ http://dx.doi.org/10.1097/01.WOX.0000411834.23118.10 Text en Copyright © 2012 by World Allergy Organization |
spellingShingle | Abstracts of the XXII World Allergy Congress Kucera, Petr Hulikova, Katarina Cvackova, Milada Planska, Daniela Riegerova, Kamila 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells? |
title | 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells? |
title_full | 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells? |
title_fullStr | 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells? |
title_full_unstemmed | 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells? |
title_short | 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells? |
title_sort | 89 is basophil specific response to hymenoptera venom related to t regulatory cells? |
topic | Abstracts of the XXII World Allergy Congress |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3512905/ http://dx.doi.org/10.1097/01.WOX.0000411834.23118.10 |
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