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89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells?

BACKGROUND: The exact mechanism of systemic hypersensitivity to venom is not exactly understood. It is suggested T cells with regulatory potential can downregulate other T cell subsets and effector cells, ex. mast cell or basophils. We focused on relationship of specific basophil reactivity in relat...

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Autores principales: Kucera, Petr, Hulikova, Katarina, Cvackova, Milada, Planska, Daniela, Riegerova, Kamila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: World Allergy Organization Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3512905/
http://dx.doi.org/10.1097/01.WOX.0000411834.23118.10
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author Kucera, Petr
Hulikova, Katarina
Cvackova, Milada
Planska, Daniela
Riegerova, Kamila
author_facet Kucera, Petr
Hulikova, Katarina
Cvackova, Milada
Planska, Daniela
Riegerova, Kamila
author_sort Kucera, Petr
collection PubMed
description BACKGROUND: The exact mechanism of systemic hypersensitivity to venom is not exactly understood. It is suggested T cells with regulatory potential can downregulate other T cell subsets and effector cells, ex. mast cell or basophils. We focused on relationship of specific basophil reactivity in relationship to proportion of regulatory T cells. METHODS: Forty-five patients with history of systemic symptoms of allergy to Hymenoptera venom were included. Basophil reactivity before the treatment and after one year of allergen immunotherapy (AIT) was measured by CD63 expression, CD203c marker used for basophil identification. Cells were stimulated by aqueous solution of allergen in concentration range 0.01 to 1 μg/mL. T regulatory cells were identified as CD4 positive, CD25 bright and CD127 negative at the same interval of treatment. Monoclonal antibodies conjugated with fluorochromes were used, measured by FACSCalibur. Paired t test and correlation analysis used for statistical evaluation. RESULTS: Median Treg proportion before therapy was 2.160, after IT 1.960, basophil specific response (proportion of CD63 positive cells) at the same interval were 3.65 and 4.11 at 0.01 μg/mL, 13.1 and 16.1 for 0.1 μg/mL and 33.85 and 40.8 for 1 μg/mL. All differences were not statistically significant. Differences of basophil activation were not significantly related to proportion of T regulatory cells. CONCLUSIONS: In our group of patients with HV allergy, treated by AIT, we did not found any relationship between basophil specific activation during allergen immunotherapy and proportion of T regulatory cells.
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spelling pubmed-35129052012-12-21 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells? Kucera, Petr Hulikova, Katarina Cvackova, Milada Planska, Daniela Riegerova, Kamila World Allergy Organ J Abstracts of the XXII World Allergy Congress BACKGROUND: The exact mechanism of systemic hypersensitivity to venom is not exactly understood. It is suggested T cells with regulatory potential can downregulate other T cell subsets and effector cells, ex. mast cell or basophils. We focused on relationship of specific basophil reactivity in relationship to proportion of regulatory T cells. METHODS: Forty-five patients with history of systemic symptoms of allergy to Hymenoptera venom were included. Basophil reactivity before the treatment and after one year of allergen immunotherapy (AIT) was measured by CD63 expression, CD203c marker used for basophil identification. Cells were stimulated by aqueous solution of allergen in concentration range 0.01 to 1 μg/mL. T regulatory cells were identified as CD4 positive, CD25 bright and CD127 negative at the same interval of treatment. Monoclonal antibodies conjugated with fluorochromes were used, measured by FACSCalibur. Paired t test and correlation analysis used for statistical evaluation. RESULTS: Median Treg proportion before therapy was 2.160, after IT 1.960, basophil specific response (proportion of CD63 positive cells) at the same interval were 3.65 and 4.11 at 0.01 μg/mL, 13.1 and 16.1 for 0.1 μg/mL and 33.85 and 40.8 for 1 μg/mL. All differences were not statistically significant. Differences of basophil activation were not significantly related to proportion of T regulatory cells. CONCLUSIONS: In our group of patients with HV allergy, treated by AIT, we did not found any relationship between basophil specific activation during allergen immunotherapy and proportion of T regulatory cells. World Allergy Organization Journal 2012-02-17 /pmc/articles/PMC3512905/ http://dx.doi.org/10.1097/01.WOX.0000411834.23118.10 Text en Copyright © 2012 by World Allergy Organization
spellingShingle Abstracts of the XXII World Allergy Congress
Kucera, Petr
Hulikova, Katarina
Cvackova, Milada
Planska, Daniela
Riegerova, Kamila
89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells?
title 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells?
title_full 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells?
title_fullStr 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells?
title_full_unstemmed 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells?
title_short 89 Is Basophil Specific Response to Hymenoptera Venom Related to T Regulatory Cells?
title_sort 89 is basophil specific response to hymenoptera venom related to t regulatory cells?
topic Abstracts of the XXII World Allergy Congress
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3512905/
http://dx.doi.org/10.1097/01.WOX.0000411834.23118.10
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