Cargando…
120 The Features of Airway Remodeling Are More Severe in Female Mice
BACKGROUND: Epidemiological studies have already shown that females are dominant in terms of the sex ratio of adult asthma prevalence and severe asthma. It has also been reported that female mice are more susceptible to the development of allergic airway inflammation and airway hyperresponsiveness (...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
World Allergy Organization Journal
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3513133/ http://dx.doi.org/10.1097/01.WOX.0000411865.43631.44 |
_version_ | 1782251883654545408 |
---|---|
author | Takeda, Masahide Ito, Wataru Ueki, Shigeharu Hirasawa, Hiroyuki Fujita, Miyoshi Konno, Yasunori Chihara, Mami Itoga, Masamichi Moritoki, Yuki Kobayashi, Yoshiki Kayaba, Hiroyuki Chihara, Junichi |
author_facet | Takeda, Masahide Ito, Wataru Ueki, Shigeharu Hirasawa, Hiroyuki Fujita, Miyoshi Konno, Yasunori Chihara, Mami Itoga, Masamichi Moritoki, Yuki Kobayashi, Yoshiki Kayaba, Hiroyuki Chihara, Junichi |
author_sort | Takeda, Masahide |
collection | PubMed |
description | BACKGROUND: Epidemiological studies have already shown that females are dominant in terms of the sex ratio of adult asthma prevalence and severe asthma. It has also been reported that female mice are more susceptible to the development of allergic airway inflammation and airway hyperresponsiveness (AHR) than males. However, there have been few reports of studies on sex difference in the pathogenesis of severe asthma, especially airway remodeling in an animal model. In this study, we investigated sex difference in formation of airway remodeling using a long-term antigen challenged asthma model. METHODS: Following ovalbumin (OVA)/alum intraperitoneal injection, male or female mice (BALB/c) were challenged with aerosolized 1% OVA on 3 days/week for 5 weeks, and we investigated the sex difference in AHR, airway inflammation, as well as airway remodeling. RESULTS: In OVA-sensitized and -challenged (OVA/OVA) female mice, AHR, the number of eosinophils and lymphocytes, as well as Th2 cytokines and growth factors in BAL fluid were increased compared with OVA/OVA male mice. On the other hand, there is no significant difference in the level of eotaxin in BAL fluid. The histological features of airway remodeling, including goblet cell hyperplasia, subepithelial fibrosis and myofibroblast hypertrophy, were also increased in OVA/OVA female mice. Moreover, serum total and OVA-specific IgE were significantly elevated in OVA/OVA female mice. CONCLUSIONS: These results indicate that female mice are dominant in terms of forming airway remodeling as compared with male mice. The involvement of sex difference for sensitization and growth factor release in lung tissue based on inflammatory cell infiltration is indicated for the mechanism of sex difference of airway remodeling. |
format | Online Article Text |
id | pubmed-3513133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | World Allergy Organization Journal |
record_format | MEDLINE/PubMed |
spelling | pubmed-35131332012-12-21 120 The Features of Airway Remodeling Are More Severe in Female Mice Takeda, Masahide Ito, Wataru Ueki, Shigeharu Hirasawa, Hiroyuki Fujita, Miyoshi Konno, Yasunori Chihara, Mami Itoga, Masamichi Moritoki, Yuki Kobayashi, Yoshiki Kayaba, Hiroyuki Chihara, Junichi World Allergy Organ J Abstracts of the XXII World Allergy Congress BACKGROUND: Epidemiological studies have already shown that females are dominant in terms of the sex ratio of adult asthma prevalence and severe asthma. It has also been reported that female mice are more susceptible to the development of allergic airway inflammation and airway hyperresponsiveness (AHR) than males. However, there have been few reports of studies on sex difference in the pathogenesis of severe asthma, especially airway remodeling in an animal model. In this study, we investigated sex difference in formation of airway remodeling using a long-term antigen challenged asthma model. METHODS: Following ovalbumin (OVA)/alum intraperitoneal injection, male or female mice (BALB/c) were challenged with aerosolized 1% OVA on 3 days/week for 5 weeks, and we investigated the sex difference in AHR, airway inflammation, as well as airway remodeling. RESULTS: In OVA-sensitized and -challenged (OVA/OVA) female mice, AHR, the number of eosinophils and lymphocytes, as well as Th2 cytokines and growth factors in BAL fluid were increased compared with OVA/OVA male mice. On the other hand, there is no significant difference in the level of eotaxin in BAL fluid. The histological features of airway remodeling, including goblet cell hyperplasia, subepithelial fibrosis and myofibroblast hypertrophy, were also increased in OVA/OVA female mice. Moreover, serum total and OVA-specific IgE were significantly elevated in OVA/OVA female mice. CONCLUSIONS: These results indicate that female mice are dominant in terms of forming airway remodeling as compared with male mice. The involvement of sex difference for sensitization and growth factor release in lung tissue based on inflammatory cell infiltration is indicated for the mechanism of sex difference of airway remodeling. World Allergy Organization Journal 2012-02-17 /pmc/articles/PMC3513133/ http://dx.doi.org/10.1097/01.WOX.0000411865.43631.44 Text en Copyright © 2012 by World Allergy Organization |
spellingShingle | Abstracts of the XXII World Allergy Congress Takeda, Masahide Ito, Wataru Ueki, Shigeharu Hirasawa, Hiroyuki Fujita, Miyoshi Konno, Yasunori Chihara, Mami Itoga, Masamichi Moritoki, Yuki Kobayashi, Yoshiki Kayaba, Hiroyuki Chihara, Junichi 120 The Features of Airway Remodeling Are More Severe in Female Mice |
title | 120 The Features of Airway Remodeling Are More Severe in Female Mice |
title_full | 120 The Features of Airway Remodeling Are More Severe in Female Mice |
title_fullStr | 120 The Features of Airway Remodeling Are More Severe in Female Mice |
title_full_unstemmed | 120 The Features of Airway Remodeling Are More Severe in Female Mice |
title_short | 120 The Features of Airway Remodeling Are More Severe in Female Mice |
title_sort | 120 the features of airway remodeling are more severe in female mice |
topic | Abstracts of the XXII World Allergy Congress |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3513133/ http://dx.doi.org/10.1097/01.WOX.0000411865.43631.44 |
work_keys_str_mv | AT takedamasahide 120thefeaturesofairwayremodelingaremoresevereinfemalemice AT itowataru 120thefeaturesofairwayremodelingaremoresevereinfemalemice AT uekishigeharu 120thefeaturesofairwayremodelingaremoresevereinfemalemice AT hirasawahiroyuki 120thefeaturesofairwayremodelingaremoresevereinfemalemice AT fujitamiyoshi 120thefeaturesofairwayremodelingaremoresevereinfemalemice AT konnoyasunori 120thefeaturesofairwayremodelingaremoresevereinfemalemice AT chiharamami 120thefeaturesofairwayremodelingaremoresevereinfemalemice AT itogamasamichi 120thefeaturesofairwayremodelingaremoresevereinfemalemice AT moritokiyuki 120thefeaturesofairwayremodelingaremoresevereinfemalemice AT kobayashiyoshiki 120thefeaturesofairwayremodelingaremoresevereinfemalemice AT kayabahiroyuki 120thefeaturesofairwayremodelingaremoresevereinfemalemice AT chiharajunichi 120thefeaturesofairwayremodelingaremoresevereinfemalemice |