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Antioxidant, Antinociceptive, and Anti-Inflammatory Activities from Actinidia callosa var. callosa In Vitro and In Vivo

Actinidia callosa var. callosa has been widely used to treat antipyretic, analgesic, anti-inflammation, abdominal pain, and fever in Taiwan. The aim of this study was to evaluate the antioxidant, antinociceptive, and anti-inflammatory lipopolysaccharide-(LPS-)induced nitric oxide (NO) production in...

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Autores principales: Liao, Jung-Chun, Deng, Jeng-Shyan, Lin, Ying-Chih, Lee, Chao-Ying, Lee, Min-Min, Hou, Wen-Chi, Huang, Shyh-Shyun, Huang, Guan-Jhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514003/
https://www.ncbi.nlm.nih.gov/pubmed/23227095
http://dx.doi.org/10.1155/2012/129152
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author Liao, Jung-Chun
Deng, Jeng-Shyan
Lin, Ying-Chih
Lee, Chao-Ying
Lee, Min-Min
Hou, Wen-Chi
Huang, Shyh-Shyun
Huang, Guan-Jhong
author_facet Liao, Jung-Chun
Deng, Jeng-Shyan
Lin, Ying-Chih
Lee, Chao-Ying
Lee, Min-Min
Hou, Wen-Chi
Huang, Shyh-Shyun
Huang, Guan-Jhong
author_sort Liao, Jung-Chun
collection PubMed
description Actinidia callosa var. callosa has been widely used to treat antipyretic, analgesic, anti-inflammation, abdominal pain, and fever in Taiwan. The aim of this study was to evaluate the antioxidant, antinociceptive, and anti-inflammatory lipopolysaccharide-(LPS-)induced nitric oxide (NO) production in RAW264.7 macrophages and pawedema induced by λ-carrageenan activities of the methanol extract from A. callosa. In HPLC analysis, the fingerprint chromatogram of ethyl-acetate fraction of A. callosa (EAAC) was established. EAAC showed the highest TEAC and DPPH radical scavenging activities, respectively. We evaluated that EAAC and the reference compound of catechin and caffeic acid decreased the LPS-induced NO production in RAW264.7 cells. Treatment of male ICR mice with EAAC significantly inhibited the numbers of acetic acid-induced writhing response and the formalin-induced pain in the late phase. Administration of EAAC showed a concentration-dependent inhibition on paw edema development after Carr treatment in mice. Anti-inflammatory mechanisms of EAAC might be correlated to the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and heme oxygenase-1 (HO-1) in vitro and in vivo. Overall, the results showed that EAAC demonstrated antioxidant, antinociceptive, and anti-inflammatory activity, which supports previous claims of the traditional use for inflammation and pain.
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spelling pubmed-35140032012-12-07 Antioxidant, Antinociceptive, and Anti-Inflammatory Activities from Actinidia callosa var. callosa In Vitro and In Vivo Liao, Jung-Chun Deng, Jeng-Shyan Lin, Ying-Chih Lee, Chao-Ying Lee, Min-Min Hou, Wen-Chi Huang, Shyh-Shyun Huang, Guan-Jhong Evid Based Complement Alternat Med Research Article Actinidia callosa var. callosa has been widely used to treat antipyretic, analgesic, anti-inflammation, abdominal pain, and fever in Taiwan. The aim of this study was to evaluate the antioxidant, antinociceptive, and anti-inflammatory lipopolysaccharide-(LPS-)induced nitric oxide (NO) production in RAW264.7 macrophages and pawedema induced by λ-carrageenan activities of the methanol extract from A. callosa. In HPLC analysis, the fingerprint chromatogram of ethyl-acetate fraction of A. callosa (EAAC) was established. EAAC showed the highest TEAC and DPPH radical scavenging activities, respectively. We evaluated that EAAC and the reference compound of catechin and caffeic acid decreased the LPS-induced NO production in RAW264.7 cells. Treatment of male ICR mice with EAAC significantly inhibited the numbers of acetic acid-induced writhing response and the formalin-induced pain in the late phase. Administration of EAAC showed a concentration-dependent inhibition on paw edema development after Carr treatment in mice. Anti-inflammatory mechanisms of EAAC might be correlated to the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and heme oxygenase-1 (HO-1) in vitro and in vivo. Overall, the results showed that EAAC demonstrated antioxidant, antinociceptive, and anti-inflammatory activity, which supports previous claims of the traditional use for inflammation and pain. Hindawi Publishing Corporation 2012 2012-11-18 /pmc/articles/PMC3514003/ /pubmed/23227095 http://dx.doi.org/10.1155/2012/129152 Text en Copyright © 2012 Jung-Chun Liao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liao, Jung-Chun
Deng, Jeng-Shyan
Lin, Ying-Chih
Lee, Chao-Ying
Lee, Min-Min
Hou, Wen-Chi
Huang, Shyh-Shyun
Huang, Guan-Jhong
Antioxidant, Antinociceptive, and Anti-Inflammatory Activities from Actinidia callosa var. callosa In Vitro and In Vivo
title Antioxidant, Antinociceptive, and Anti-Inflammatory Activities from Actinidia callosa var. callosa In Vitro and In Vivo
title_full Antioxidant, Antinociceptive, and Anti-Inflammatory Activities from Actinidia callosa var. callosa In Vitro and In Vivo
title_fullStr Antioxidant, Antinociceptive, and Anti-Inflammatory Activities from Actinidia callosa var. callosa In Vitro and In Vivo
title_full_unstemmed Antioxidant, Antinociceptive, and Anti-Inflammatory Activities from Actinidia callosa var. callosa In Vitro and In Vivo
title_short Antioxidant, Antinociceptive, and Anti-Inflammatory Activities from Actinidia callosa var. callosa In Vitro and In Vivo
title_sort antioxidant, antinociceptive, and anti-inflammatory activities from actinidia callosa var. callosa in vitro and in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514003/
https://www.ncbi.nlm.nih.gov/pubmed/23227095
http://dx.doi.org/10.1155/2012/129152
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