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Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission
Fungal cytokinesis requires the assembly of a dividing septum wall. In yeast, the septum has to be selectively digested during the critical cell separation process. Fission yeast cell wall α(1-3)glucan is essential, but nothing is known about its localization and function in the cell wall or about c...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514033/ https://www.ncbi.nlm.nih.gov/pubmed/22891259 http://dx.doi.org/10.1083/jcb.201202015 |
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author | Cortés, Juan Carlos G. Sato, Mamiko Muñoz, Javier Moreno, M. Belén Clemente-Ramos, Jose Angel Ramos, Mariona Okada, Hitoshi Osumi, Masako Durán, Angel Ribas, Juan Carlos |
author_facet | Cortés, Juan Carlos G. Sato, Mamiko Muñoz, Javier Moreno, M. Belén Clemente-Ramos, Jose Angel Ramos, Mariona Okada, Hitoshi Osumi, Masako Durán, Angel Ribas, Juan Carlos |
author_sort | Cortés, Juan Carlos G. |
collection | PubMed |
description | Fungal cytokinesis requires the assembly of a dividing septum wall. In yeast, the septum has to be selectively digested during the critical cell separation process. Fission yeast cell wall α(1-3)glucan is essential, but nothing is known about its localization and function in the cell wall or about cooperation between the α- and β(1-3)glucan synthases Ags1 and Bgs for cell wall and septum assembly. Here, we generate a physiological Ags1-GFP variant and demonstrate a tight colocalization with Bgs1, suggesting a cooperation in the important early steps of septum construction. Moreover, we define the essential functions of α(1-3)glucan in septation and cell separation. We show that α(1-3)glucan is essential for both secondary septum formation and the primary septum structural strength needed to support the physical forces of the cell turgor pressure during cell separation. Consequently, the absence of Ags1 and therefore α(1-3)glucan generates a special and unique side-explosive cell separation due to an instantaneous primary septum tearing caused by the turgor pressure. |
format | Online Article Text |
id | pubmed-3514033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-35140332013-02-20 Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission Cortés, Juan Carlos G. Sato, Mamiko Muñoz, Javier Moreno, M. Belén Clemente-Ramos, Jose Angel Ramos, Mariona Okada, Hitoshi Osumi, Masako Durán, Angel Ribas, Juan Carlos J Cell Biol Research Articles Fungal cytokinesis requires the assembly of a dividing septum wall. In yeast, the septum has to be selectively digested during the critical cell separation process. Fission yeast cell wall α(1-3)glucan is essential, but nothing is known about its localization and function in the cell wall or about cooperation between the α- and β(1-3)glucan synthases Ags1 and Bgs for cell wall and septum assembly. Here, we generate a physiological Ags1-GFP variant and demonstrate a tight colocalization with Bgs1, suggesting a cooperation in the important early steps of septum construction. Moreover, we define the essential functions of α(1-3)glucan in septation and cell separation. We show that α(1-3)glucan is essential for both secondary septum formation and the primary septum structural strength needed to support the physical forces of the cell turgor pressure during cell separation. Consequently, the absence of Ags1 and therefore α(1-3)glucan generates a special and unique side-explosive cell separation due to an instantaneous primary septum tearing caused by the turgor pressure. The Rockefeller University Press 2012-08-20 /pmc/articles/PMC3514033/ /pubmed/22891259 http://dx.doi.org/10.1083/jcb.201202015 Text en © 2012 Cortés et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Cortés, Juan Carlos G. Sato, Mamiko Muñoz, Javier Moreno, M. Belén Clemente-Ramos, Jose Angel Ramos, Mariona Okada, Hitoshi Osumi, Masako Durán, Angel Ribas, Juan Carlos Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission |
title | Fission yeast Ags1 confers the essential septum strength needed for
safe gradual cell abscission |
title_full | Fission yeast Ags1 confers the essential septum strength needed for
safe gradual cell abscission |
title_fullStr | Fission yeast Ags1 confers the essential septum strength needed for
safe gradual cell abscission |
title_full_unstemmed | Fission yeast Ags1 confers the essential septum strength needed for
safe gradual cell abscission |
title_short | Fission yeast Ags1 confers the essential septum strength needed for
safe gradual cell abscission |
title_sort | fission yeast ags1 confers the essential septum strength needed for
safe gradual cell abscission |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514033/ https://www.ncbi.nlm.nih.gov/pubmed/22891259 http://dx.doi.org/10.1083/jcb.201202015 |
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