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Human Haemato-Endothelial Precursors: Cord Blood CD34+ Cells Produce Haemogenic Endothelium

Embryologic and genetic evidence suggest a common origin of haematopoietic and endothelial lineages. In the murine embryo, recent studies indicate the presence of haemogenic endothelium and of a common haemato-endothelial precursor, the haemangioblast. Conversely, so far, little evidence supports th...

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Autores principales: Pelosi, Elvira, Castelli, Germana, Martin-Padura, Ines, Bordoni, Veronica, Santoro, Simona, Conigliaro, Alice, Cerio, Anna Maria, De Santis Puzzonia, Marco, Marighetti, Paola, Biffoni, Mauro, Alonzi, Tonino, Amicone, Laura, Alcalay, Myriam, Bertolini, Francesco, Testa, Ugo, Tripodi, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514182/
https://www.ncbi.nlm.nih.gov/pubmed/23226561
http://dx.doi.org/10.1371/journal.pone.0051109
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author Pelosi, Elvira
Castelli, Germana
Martin-Padura, Ines
Bordoni, Veronica
Santoro, Simona
Conigliaro, Alice
Cerio, Anna Maria
De Santis Puzzonia, Marco
Marighetti, Paola
Biffoni, Mauro
Alonzi, Tonino
Amicone, Laura
Alcalay, Myriam
Bertolini, Francesco
Testa, Ugo
Tripodi, Marco
author_facet Pelosi, Elvira
Castelli, Germana
Martin-Padura, Ines
Bordoni, Veronica
Santoro, Simona
Conigliaro, Alice
Cerio, Anna Maria
De Santis Puzzonia, Marco
Marighetti, Paola
Biffoni, Mauro
Alonzi, Tonino
Amicone, Laura
Alcalay, Myriam
Bertolini, Francesco
Testa, Ugo
Tripodi, Marco
author_sort Pelosi, Elvira
collection PubMed
description Embryologic and genetic evidence suggest a common origin of haematopoietic and endothelial lineages. In the murine embryo, recent studies indicate the presence of haemogenic endothelium and of a common haemato-endothelial precursor, the haemangioblast. Conversely, so far, little evidence supports the presence of haemogenic endothelium and haemangioblasts in later stages of development. Our studies indicate that human cord blood haematopoietic progenitors (CD34+45+144−), triggered by murine hepatocyte conditioned medium, differentiate into adherent proliferating endothelial precursors (CD144+CD105+CD146+CD31+CD45−) capable of functioning as haemogenic endothelium. These cells, proven to give rise to functional vasculature in vivo, if further instructed by haematopoietic growth factors, first switch to transitional CD144+45+ cells and then to haematopoietic cells. These results highlight the plasticity of haemato-endhothelial precursors in human post-natal life. Furthermore, these studies may provide highly enriched populations of human post-fetal haemogenic endothelium, paving the way for innovative projects at a basic and possibly clinical level.
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spelling pubmed-35141822012-12-05 Human Haemato-Endothelial Precursors: Cord Blood CD34+ Cells Produce Haemogenic Endothelium Pelosi, Elvira Castelli, Germana Martin-Padura, Ines Bordoni, Veronica Santoro, Simona Conigliaro, Alice Cerio, Anna Maria De Santis Puzzonia, Marco Marighetti, Paola Biffoni, Mauro Alonzi, Tonino Amicone, Laura Alcalay, Myriam Bertolini, Francesco Testa, Ugo Tripodi, Marco PLoS One Research Article Embryologic and genetic evidence suggest a common origin of haematopoietic and endothelial lineages. In the murine embryo, recent studies indicate the presence of haemogenic endothelium and of a common haemato-endothelial precursor, the haemangioblast. Conversely, so far, little evidence supports the presence of haemogenic endothelium and haemangioblasts in later stages of development. Our studies indicate that human cord blood haematopoietic progenitors (CD34+45+144−), triggered by murine hepatocyte conditioned medium, differentiate into adherent proliferating endothelial precursors (CD144+CD105+CD146+CD31+CD45−) capable of functioning as haemogenic endothelium. These cells, proven to give rise to functional vasculature in vivo, if further instructed by haematopoietic growth factors, first switch to transitional CD144+45+ cells and then to haematopoietic cells. These results highlight the plasticity of haemato-endhothelial precursors in human post-natal life. Furthermore, these studies may provide highly enriched populations of human post-fetal haemogenic endothelium, paving the way for innovative projects at a basic and possibly clinical level. Public Library of Science 2012-12-04 /pmc/articles/PMC3514182/ /pubmed/23226561 http://dx.doi.org/10.1371/journal.pone.0051109 Text en © 2012 Pelosi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pelosi, Elvira
Castelli, Germana
Martin-Padura, Ines
Bordoni, Veronica
Santoro, Simona
Conigliaro, Alice
Cerio, Anna Maria
De Santis Puzzonia, Marco
Marighetti, Paola
Biffoni, Mauro
Alonzi, Tonino
Amicone, Laura
Alcalay, Myriam
Bertolini, Francesco
Testa, Ugo
Tripodi, Marco
Human Haemato-Endothelial Precursors: Cord Blood CD34+ Cells Produce Haemogenic Endothelium
title Human Haemato-Endothelial Precursors: Cord Blood CD34+ Cells Produce Haemogenic Endothelium
title_full Human Haemato-Endothelial Precursors: Cord Blood CD34+ Cells Produce Haemogenic Endothelium
title_fullStr Human Haemato-Endothelial Precursors: Cord Blood CD34+ Cells Produce Haemogenic Endothelium
title_full_unstemmed Human Haemato-Endothelial Precursors: Cord Blood CD34+ Cells Produce Haemogenic Endothelium
title_short Human Haemato-Endothelial Precursors: Cord Blood CD34+ Cells Produce Haemogenic Endothelium
title_sort human haemato-endothelial precursors: cord blood cd34+ cells produce haemogenic endothelium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514182/
https://www.ncbi.nlm.nih.gov/pubmed/23226561
http://dx.doi.org/10.1371/journal.pone.0051109
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