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Extract of Kuding Tea Prevents High-Fat Diet-Induced Metabolic Disorders in C57BL/6 Mice via Liver X Receptor (LXR) β Antagonism

OBJECTIVE: To investigate the effects of ilex kudingcha C. J. Tseng (kuding tea), a traditional beverage in China, on the metabolic disorders in C57BL/6 mice induced by high-fat diets. DESIGN: For the preventive experiment, the female C57BL/6 mice were fed with a standard diet (Chow), high-fat diet...

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Autores principales: Fan, Shengjie, Zhang, Yu, Hu, Na, Sun, Qinhu, Ding, Xiaobo, Li, Guowen, Zheng, Bin, Gu, Ming, Huang, Feisi, Sun, Yin-Qiang, Zhou, Zhiqin, Lu, Xiong, Huang, Cheng, Ji, Guang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514219/
https://www.ncbi.nlm.nih.gov/pubmed/23226556
http://dx.doi.org/10.1371/journal.pone.0051007
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author Fan, Shengjie
Zhang, Yu
Hu, Na
Sun, Qinhu
Ding, Xiaobo
Li, Guowen
Zheng, Bin
Gu, Ming
Huang, Feisi
Sun, Yin-Qiang
Zhou, Zhiqin
Lu, Xiong
Huang, Cheng
Ji, Guang
author_facet Fan, Shengjie
Zhang, Yu
Hu, Na
Sun, Qinhu
Ding, Xiaobo
Li, Guowen
Zheng, Bin
Gu, Ming
Huang, Feisi
Sun, Yin-Qiang
Zhou, Zhiqin
Lu, Xiong
Huang, Cheng
Ji, Guang
author_sort Fan, Shengjie
collection PubMed
description OBJECTIVE: To investigate the effects of ilex kudingcha C. J. Tseng (kuding tea), a traditional beverage in China, on the metabolic disorders in C57BL/6 mice induced by high-fat diets. DESIGN: For the preventive experiment, the female C57BL/6 mice were fed with a standard diet (Chow), high-fat diet (HF), and high-fat diet mixed with 0.05% ethanol extract of kuding tea (EK) for 5 weeks. For the therapeutic experiment, the C57BL/6 mice were fed high-fat diet for 3 months, and then mice were split and EK was given with oral gavages for 2 weeks at 50 mg/day/kg. Body weight and daily food intake amounts were measured. At the end of treatment, the adipocyte images were assayed with a scanning electron microscope, and the fasting blood glucose, glucose tolerance test, serum lipid profile and lipids in the livers were analyzed. A reporter gene assay system was used to test the whether EK could act on nuclear receptor transcription factors, and the gene expression analysis was performed with a quantitative PCR assay. RESULTS: In the preventive treatment, EK blocked the body weight gain, reduced the size of the adipocytes, lowered serum triglyceride, cholesterol, LDL-cholesterol, fasting blood glucose levels and glucose tolerance in high-fat diet-fed C57BL/6 mice. In the therapeutic treatment, EK reduced the size of the white adipocytes, serum TG and fasting blood glucose levels in obese mice. With the reporter assay, EK inhibited LXRβ transactivity and mRNA expression of LXRβ target genes. CONCLUSION: We observed that EK has both preventive and therapeutic roles in metabolic disorders in mice induced with high-fat diets. The effects appear to be mediated through the antagonism of LXRβ transactivity. Our data indicate that kuding tea is a useful dietary therapy and a potential source for the development of novel anti-obesity and lipid lowering drugs.
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spelling pubmed-35142192012-12-05 Extract of Kuding Tea Prevents High-Fat Diet-Induced Metabolic Disorders in C57BL/6 Mice via Liver X Receptor (LXR) β Antagonism Fan, Shengjie Zhang, Yu Hu, Na Sun, Qinhu Ding, Xiaobo Li, Guowen Zheng, Bin Gu, Ming Huang, Feisi Sun, Yin-Qiang Zhou, Zhiqin Lu, Xiong Huang, Cheng Ji, Guang PLoS One Research Article OBJECTIVE: To investigate the effects of ilex kudingcha C. J. Tseng (kuding tea), a traditional beverage in China, on the metabolic disorders in C57BL/6 mice induced by high-fat diets. DESIGN: For the preventive experiment, the female C57BL/6 mice were fed with a standard diet (Chow), high-fat diet (HF), and high-fat diet mixed with 0.05% ethanol extract of kuding tea (EK) for 5 weeks. For the therapeutic experiment, the C57BL/6 mice were fed high-fat diet for 3 months, and then mice were split and EK was given with oral gavages for 2 weeks at 50 mg/day/kg. Body weight and daily food intake amounts were measured. At the end of treatment, the adipocyte images were assayed with a scanning electron microscope, and the fasting blood glucose, glucose tolerance test, serum lipid profile and lipids in the livers were analyzed. A reporter gene assay system was used to test the whether EK could act on nuclear receptor transcription factors, and the gene expression analysis was performed with a quantitative PCR assay. RESULTS: In the preventive treatment, EK blocked the body weight gain, reduced the size of the adipocytes, lowered serum triglyceride, cholesterol, LDL-cholesterol, fasting blood glucose levels and glucose tolerance in high-fat diet-fed C57BL/6 mice. In the therapeutic treatment, EK reduced the size of the white adipocytes, serum TG and fasting blood glucose levels in obese mice. With the reporter assay, EK inhibited LXRβ transactivity and mRNA expression of LXRβ target genes. CONCLUSION: We observed that EK has both preventive and therapeutic roles in metabolic disorders in mice induced with high-fat diets. The effects appear to be mediated through the antagonism of LXRβ transactivity. Our data indicate that kuding tea is a useful dietary therapy and a potential source for the development of novel anti-obesity and lipid lowering drugs. Public Library of Science 2012-12-04 /pmc/articles/PMC3514219/ /pubmed/23226556 http://dx.doi.org/10.1371/journal.pone.0051007 Text en © 2012 Fan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fan, Shengjie
Zhang, Yu
Hu, Na
Sun, Qinhu
Ding, Xiaobo
Li, Guowen
Zheng, Bin
Gu, Ming
Huang, Feisi
Sun, Yin-Qiang
Zhou, Zhiqin
Lu, Xiong
Huang, Cheng
Ji, Guang
Extract of Kuding Tea Prevents High-Fat Diet-Induced Metabolic Disorders in C57BL/6 Mice via Liver X Receptor (LXR) β Antagonism
title Extract of Kuding Tea Prevents High-Fat Diet-Induced Metabolic Disorders in C57BL/6 Mice via Liver X Receptor (LXR) β Antagonism
title_full Extract of Kuding Tea Prevents High-Fat Diet-Induced Metabolic Disorders in C57BL/6 Mice via Liver X Receptor (LXR) β Antagonism
title_fullStr Extract of Kuding Tea Prevents High-Fat Diet-Induced Metabolic Disorders in C57BL/6 Mice via Liver X Receptor (LXR) β Antagonism
title_full_unstemmed Extract of Kuding Tea Prevents High-Fat Diet-Induced Metabolic Disorders in C57BL/6 Mice via Liver X Receptor (LXR) β Antagonism
title_short Extract of Kuding Tea Prevents High-Fat Diet-Induced Metabolic Disorders in C57BL/6 Mice via Liver X Receptor (LXR) β Antagonism
title_sort extract of kuding tea prevents high-fat diet-induced metabolic disorders in c57bl/6 mice via liver x receptor (lxr) β antagonism
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514219/
https://www.ncbi.nlm.nih.gov/pubmed/23226556
http://dx.doi.org/10.1371/journal.pone.0051007
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