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Engineered Repressible Lethality for Controlling the Pink Bollworm, a Lepidopteran Pest of Cotton

The sterile insect technique (SIT) is an environmentally friendly method of pest control in which insects are mass-produced, irradiated and released to mate with wild counterparts. SIT has been used to control major pest insects including the pink bollworm (Pectinophora gossypiella Saunders), a glob...

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Autores principales: Morrison, Neil I., Simmons, Gregory S., Fu, Guoliang, O’Connell, Sinead, Walker, Adam S., Dafa’alla, Tarig, Walters, Michelle, Claus, John, Tang, Guolei, Jin, Li, Marubbi, Thea, Epton, Matthew J., Harris, Claire L., Staten, Robert T., Miller, Ernest, Miller, Thomas A., Alphey, Luke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514271/
https://www.ncbi.nlm.nih.gov/pubmed/23226548
http://dx.doi.org/10.1371/journal.pone.0050922
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author Morrison, Neil I.
Simmons, Gregory S.
Fu, Guoliang
O’Connell, Sinead
Walker, Adam S.
Dafa’alla, Tarig
Walters, Michelle
Claus, John
Tang, Guolei
Jin, Li
Marubbi, Thea
Epton, Matthew J.
Harris, Claire L.
Staten, Robert T.
Miller, Ernest
Miller, Thomas A.
Alphey, Luke
author_facet Morrison, Neil I.
Simmons, Gregory S.
Fu, Guoliang
O’Connell, Sinead
Walker, Adam S.
Dafa’alla, Tarig
Walters, Michelle
Claus, John
Tang, Guolei
Jin, Li
Marubbi, Thea
Epton, Matthew J.
Harris, Claire L.
Staten, Robert T.
Miller, Ernest
Miller, Thomas A.
Alphey, Luke
author_sort Morrison, Neil I.
collection PubMed
description The sterile insect technique (SIT) is an environmentally friendly method of pest control in which insects are mass-produced, irradiated and released to mate with wild counterparts. SIT has been used to control major pest insects including the pink bollworm (Pectinophora gossypiella Saunders), a global pest of cotton. Transgenic technology has the potential to overcome disadvantages associated with the SIT, such as the damaging effects of radiation on released insects. A method called RIDL (Release of Insects carrying a Dominant Lethal) is designed to circumvent the need to irradiate insects before release. Premature death of insects’ progeny can be engineered to provide an equivalent to sterilisation. Moreover, this trait can be suppressed by the provision of a dietary antidote. In the pink bollworm, we generated transformed strains using different DNA constructs, which showed moderate-to-100% engineered mortality. In permissive conditions, this effect was largely suppressed. Survival data on cotton in field cages indicated that field conditions increase the lethal effect. One strain, called OX3402C, showed highly penetrant and highly repressible lethality, and was tested on host plants where its larvae caused minimal damage before death. These results highlight a potentially valuable insecticide-free tool against pink bollworm, and indicate its potential for development in other lepidopteran pests.
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spelling pubmed-35142712012-12-05 Engineered Repressible Lethality for Controlling the Pink Bollworm, a Lepidopteran Pest of Cotton Morrison, Neil I. Simmons, Gregory S. Fu, Guoliang O’Connell, Sinead Walker, Adam S. Dafa’alla, Tarig Walters, Michelle Claus, John Tang, Guolei Jin, Li Marubbi, Thea Epton, Matthew J. Harris, Claire L. Staten, Robert T. Miller, Ernest Miller, Thomas A. Alphey, Luke PLoS One Research Article The sterile insect technique (SIT) is an environmentally friendly method of pest control in which insects are mass-produced, irradiated and released to mate with wild counterparts. SIT has been used to control major pest insects including the pink bollworm (Pectinophora gossypiella Saunders), a global pest of cotton. Transgenic technology has the potential to overcome disadvantages associated with the SIT, such as the damaging effects of radiation on released insects. A method called RIDL (Release of Insects carrying a Dominant Lethal) is designed to circumvent the need to irradiate insects before release. Premature death of insects’ progeny can be engineered to provide an equivalent to sterilisation. Moreover, this trait can be suppressed by the provision of a dietary antidote. In the pink bollworm, we generated transformed strains using different DNA constructs, which showed moderate-to-100% engineered mortality. In permissive conditions, this effect was largely suppressed. Survival data on cotton in field cages indicated that field conditions increase the lethal effect. One strain, called OX3402C, showed highly penetrant and highly repressible lethality, and was tested on host plants where its larvae caused minimal damage before death. These results highlight a potentially valuable insecticide-free tool against pink bollworm, and indicate its potential for development in other lepidopteran pests. Public Library of Science 2012-12-04 /pmc/articles/PMC3514271/ /pubmed/23226548 http://dx.doi.org/10.1371/journal.pone.0050922 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Morrison, Neil I.
Simmons, Gregory S.
Fu, Guoliang
O’Connell, Sinead
Walker, Adam S.
Dafa’alla, Tarig
Walters, Michelle
Claus, John
Tang, Guolei
Jin, Li
Marubbi, Thea
Epton, Matthew J.
Harris, Claire L.
Staten, Robert T.
Miller, Ernest
Miller, Thomas A.
Alphey, Luke
Engineered Repressible Lethality for Controlling the Pink Bollworm, a Lepidopteran Pest of Cotton
title Engineered Repressible Lethality for Controlling the Pink Bollworm, a Lepidopteran Pest of Cotton
title_full Engineered Repressible Lethality for Controlling the Pink Bollworm, a Lepidopteran Pest of Cotton
title_fullStr Engineered Repressible Lethality for Controlling the Pink Bollworm, a Lepidopteran Pest of Cotton
title_full_unstemmed Engineered Repressible Lethality for Controlling the Pink Bollworm, a Lepidopteran Pest of Cotton
title_short Engineered Repressible Lethality for Controlling the Pink Bollworm, a Lepidopteran Pest of Cotton
title_sort engineered repressible lethality for controlling the pink bollworm, a lepidopteran pest of cotton
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514271/
https://www.ncbi.nlm.nih.gov/pubmed/23226548
http://dx.doi.org/10.1371/journal.pone.0050922
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