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A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons

Chromosome rearrangements in small apes are up to 20 times more frequent than in most mammals. Because of their complexity, the full extent of chromosome evolution in these hominoids is not yet fully documented. However, previous work with array painting, BAC-FISH, and selective sequencing in two of...

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Autores principales: Capozzi, Oronzo, Carbone, Lucia, Stanyon, Roscoe R., Marra, Annamaria, Yang, Fengtang, Whelan, Christopher W., de Jong, Pieter J., Rocchi, Mariano, Archidiacono, Nicoletta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514681/
https://www.ncbi.nlm.nih.gov/pubmed/22892276
http://dx.doi.org/10.1101/gr.138651.112
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author Capozzi, Oronzo
Carbone, Lucia
Stanyon, Roscoe R.
Marra, Annamaria
Yang, Fengtang
Whelan, Christopher W.
de Jong, Pieter J.
Rocchi, Mariano
Archidiacono, Nicoletta
author_facet Capozzi, Oronzo
Carbone, Lucia
Stanyon, Roscoe R.
Marra, Annamaria
Yang, Fengtang
Whelan, Christopher W.
de Jong, Pieter J.
Rocchi, Mariano
Archidiacono, Nicoletta
author_sort Capozzi, Oronzo
collection PubMed
description Chromosome rearrangements in small apes are up to 20 times more frequent than in most mammals. Because of their complexity, the full extent of chromosome evolution in these hominoids is not yet fully documented. However, previous work with array painting, BAC-FISH, and selective sequencing in two of the four karyomorphs has shown that high-resolution methods can precisely define chromosome breakpoints and map the complex flow of evolutionary chromosome rearrangements. Here we use these tools to precisely define the rearrangements that have occurred in the remaining two karyomorphs, genera Symphalangus (2n = 50) and Hoolock (2n = 38). This research provides the most comprehensive insight into the evolutionary origins of chromosome rearrangements involved in transforming small apes genome. Bioinformatics analyses of the human–gibbon synteny breakpoints revealed association with transposable elements and segmental duplications, providing some insight into the mechanisms that might have promoted rearrangements in small apes. In the near future, the comparison of gibbon genome sequences will provide novel insights to test hypotheses concerning the mechanisms of chromosome evolution. The precise definition of synteny block boundaries and orientation, chromosomal fusions, and centromere repositioning events presented here will facilitate genome sequence assembly for these close relatives of humans.
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spelling pubmed-35146812013-06-01 A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons Capozzi, Oronzo Carbone, Lucia Stanyon, Roscoe R. Marra, Annamaria Yang, Fengtang Whelan, Christopher W. de Jong, Pieter J. Rocchi, Mariano Archidiacono, Nicoletta Genome Res Resource Chromosome rearrangements in small apes are up to 20 times more frequent than in most mammals. Because of their complexity, the full extent of chromosome evolution in these hominoids is not yet fully documented. However, previous work with array painting, BAC-FISH, and selective sequencing in two of the four karyomorphs has shown that high-resolution methods can precisely define chromosome breakpoints and map the complex flow of evolutionary chromosome rearrangements. Here we use these tools to precisely define the rearrangements that have occurred in the remaining two karyomorphs, genera Symphalangus (2n = 50) and Hoolock (2n = 38). This research provides the most comprehensive insight into the evolutionary origins of chromosome rearrangements involved in transforming small apes genome. Bioinformatics analyses of the human–gibbon synteny breakpoints revealed association with transposable elements and segmental duplications, providing some insight into the mechanisms that might have promoted rearrangements in small apes. In the near future, the comparison of gibbon genome sequences will provide novel insights to test hypotheses concerning the mechanisms of chromosome evolution. The precise definition of synteny block boundaries and orientation, chromosomal fusions, and centromere repositioning events presented here will facilitate genome sequence assembly for these close relatives of humans. Cold Spring Harbor Laboratory Press 2012-12 /pmc/articles/PMC3514681/ /pubmed/22892276 http://dx.doi.org/10.1101/gr.138651.112 Text en © 2012, Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.
spellingShingle Resource
Capozzi, Oronzo
Carbone, Lucia
Stanyon, Roscoe R.
Marra, Annamaria
Yang, Fengtang
Whelan, Christopher W.
de Jong, Pieter J.
Rocchi, Mariano
Archidiacono, Nicoletta
A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons
title A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons
title_full A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons
title_fullStr A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons
title_full_unstemmed A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons
title_short A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons
title_sort comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons
topic Resource
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514681/
https://www.ncbi.nlm.nih.gov/pubmed/22892276
http://dx.doi.org/10.1101/gr.138651.112
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