Cargando…
Microsatellites grant more stable flanking genes
BACKGROUND: Microsatellites, or simple sequence repeats (SSRs), are DNA sequences that include tandem copies of specific sequences no longer than six bases. SSRs are ubiquitous in all genomes and highly mutable. PRESENTATION OF THE HYPOTHESIS: Results from previous studies suggest that flanking regi...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515467/ https://www.ncbi.nlm.nih.gov/pubmed/23035963 http://dx.doi.org/10.1186/1756-0500-5-556 |
_version_ | 1782252186788429824 |
---|---|
author | Joukhadar, Reem Jighly, Abdulqader |
author_facet | Joukhadar, Reem Jighly, Abdulqader |
author_sort | Joukhadar, Reem |
collection | PubMed |
description | BACKGROUND: Microsatellites, or simple sequence repeats (SSRs), are DNA sequences that include tandem copies of specific sequences no longer than six bases. SSRs are ubiquitous in all genomes and highly mutable. PRESENTATION OF THE HYPOTHESIS: Results from previous studies suggest that flanking regions of SSR are exhibit high stability in a wide range of organisms. We hypothesized that the SSRs ability to discard weak DNA polymerases could be responsible for this unusual stability. . When the weak polymerases are being decayed over SSRs, the flanking sequences would have higher opportunity to be replicated by more stable DNA polymerases. We present evidence of the molecular basis of our hypothesis. TESTING THE HYPOTHESIS: The hypothesis could be tested by examining the activity of DNA polymerase during and after a number of PCRs. The PCR reactions should be run with the same SSR locus possessing differences in the SSR length. The hypothesis could also be tested by comparing the mutational rate of a transferred gene between two transformations. The first one has a naked T-DNA (transferred DNA), while the second one has the same T-DNA flanked with two SSRs. IMPLICATIONS OF THE HYPOTHESIS: In any transformation experiment, flanking the T-DNA fragment with SSR sequences would result in more stably transferred genes. This process would decrease the unpredictable risks that may occur because of the mutational pressure on this foreign segment. |
format | Online Article Text |
id | pubmed-3515467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35154672012-12-06 Microsatellites grant more stable flanking genes Joukhadar, Reem Jighly, Abdulqader BMC Res Notes Hypothesis BACKGROUND: Microsatellites, or simple sequence repeats (SSRs), are DNA sequences that include tandem copies of specific sequences no longer than six bases. SSRs are ubiquitous in all genomes and highly mutable. PRESENTATION OF THE HYPOTHESIS: Results from previous studies suggest that flanking regions of SSR are exhibit high stability in a wide range of organisms. We hypothesized that the SSRs ability to discard weak DNA polymerases could be responsible for this unusual stability. . When the weak polymerases are being decayed over SSRs, the flanking sequences would have higher opportunity to be replicated by more stable DNA polymerases. We present evidence of the molecular basis of our hypothesis. TESTING THE HYPOTHESIS: The hypothesis could be tested by examining the activity of DNA polymerase during and after a number of PCRs. The PCR reactions should be run with the same SSR locus possessing differences in the SSR length. The hypothesis could also be tested by comparing the mutational rate of a transferred gene between two transformations. The first one has a naked T-DNA (transferred DNA), while the second one has the same T-DNA flanked with two SSRs. IMPLICATIONS OF THE HYPOTHESIS: In any transformation experiment, flanking the T-DNA fragment with SSR sequences would result in more stably transferred genes. This process would decrease the unpredictable risks that may occur because of the mutational pressure on this foreign segment. BioMed Central 2012-10-05 /pmc/articles/PMC3515467/ /pubmed/23035963 http://dx.doi.org/10.1186/1756-0500-5-556 Text en Copyright ©2012 Joukhadar and Jighly; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Hypothesis Joukhadar, Reem Jighly, Abdulqader Microsatellites grant more stable flanking genes |
title | Microsatellites grant more stable flanking genes |
title_full | Microsatellites grant more stable flanking genes |
title_fullStr | Microsatellites grant more stable flanking genes |
title_full_unstemmed | Microsatellites grant more stable flanking genes |
title_short | Microsatellites grant more stable flanking genes |
title_sort | microsatellites grant more stable flanking genes |
topic | Hypothesis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515467/ https://www.ncbi.nlm.nih.gov/pubmed/23035963 http://dx.doi.org/10.1186/1756-0500-5-556 |
work_keys_str_mv | AT joukhadarreem microsatellitesgrantmorestableflankinggenes AT jighlyabdulqader microsatellitesgrantmorestableflankinggenes |