Cargando…

Proteomic Approaches Identify Members of Cofilin Pathway Involved in Oral Tumorigenesis

The prediction of tumor behavior for patients with oral carcinomas remains a challenge for clinicians. The presence of lymph node metastasis is the most important prognostic factor but it is limited in predicting local relapse or survival. This highlights the need for identifying biomarkers that may...

Descripción completa

Detalles Bibliográficos
Autores principales: Polachini, Giovana M., Sobral, Lays M., Mercante, Ana M. C., Paes-Leme, Adriana F., Xavier, Flávia C. A., Henrique, Tiago, Guimarães, Douglas M., Vidotto, Alessandra, Fukuyama, Erica E., Góis-Filho, José F., Cury, Patricia M., Curioni, Otávio A., Michaluart Jr, Pedro, Silva, Adriana M. A., Wünsch-Filho, Victor, Nunes, Fabio D., Leopoldino, Andréia M., Tajara, Eloiza H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515627/
https://www.ncbi.nlm.nih.gov/pubmed/23227181
http://dx.doi.org/10.1371/journal.pone.0050517
Descripción
Sumario:The prediction of tumor behavior for patients with oral carcinomas remains a challenge for clinicians. The presence of lymph node metastasis is the most important prognostic factor but it is limited in predicting local relapse or survival. This highlights the need for identifying biomarkers that may effectively contribute to prediction of recurrence and tumor spread. In this study, we used one- and two-dimensional gel electrophoresis, mass spectrometry and immunodetection methods to analyze protein expression in oral squamous cell carcinomas. Using a refinement for classifying oral carcinomas in regard to prognosis, we analyzed small but lymph node metastasis-positive versus large, lymph node metastasis-negative tumors in order to contribute to the molecular characterization of subgroups with risk of dissemination. Specific protein patterns favoring metastasis were observed in the “more-aggressive” group defined by the present study. This group displayed upregulation of proteins involved in migration, adhesion, angiogenesis, cell cycle regulation, anti-apoptosis and epithelial to mesenchymal transition, whereas the “less-aggressive” group was engaged in keratinocyte differentiation, epidermis development, inflammation and immune response. Besides the identification of several proteins not yet described as deregulated in oral carcinomas, the present study demonstrated for the first time the role of cofilin-1 in modulating cell invasion in oral carcinomas.