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Effects of High Fat Feeding on Liver Gene Expression in Diabetic Goto-Kakizaki Rats
Effects of high fat diet (HFD) on obesity and, subsequently, on diabetes are highly variable and modulated by genetics in both humans and rodents. In this report, we characterized the response of Goto-Kakizaki (GK) rats, a spontaneous polygenic model for lean diabetes and healthy Wistar-Kyoto (WKY)...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Libertas Academica
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3516129/ https://www.ncbi.nlm.nih.gov/pubmed/23236253 http://dx.doi.org/10.4137/GRSB.S10371 |
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author | Almon, Richard R. DuBois, Debra C. Sukumaran, Siddharth Wang, Xi Xue, Bai Nie, Jing Jusko, William J. |
author_facet | Almon, Richard R. DuBois, Debra C. Sukumaran, Siddharth Wang, Xi Xue, Bai Nie, Jing Jusko, William J. |
author_sort | Almon, Richard R. |
collection | PubMed |
description | Effects of high fat diet (HFD) on obesity and, subsequently, on diabetes are highly variable and modulated by genetics in both humans and rodents. In this report, we characterized the response of Goto-Kakizaki (GK) rats, a spontaneous polygenic model for lean diabetes and healthy Wistar-Kyoto (WKY) controls, to high fat feeding from weaning to 20 weeks of age. Animals fed either normal diet or HFD were sacrificed at 4, 8, 12, 16 and 20 weeks of age and a wide array of physiological measurements were made along with gene expression profiling using Affymetrix gene array chips. Mining of the microarray data identified differentially regulated genes (involved in inflammation, metabolism, transcription regulation, and signaling) in diabetic animals, as well as the response of both strains to HFD. Functional annotation suggested that HFD increased inflammatory differences between the two strains. Chronic inflammation driven by heightened innate immune response was identified to be present in GK animals regardless of diet. In addition, compensatory mechanisms by which WKY animals on HFD resisted the development of diabetes were identified, thus illustrating the complexity of diabetes disease progression. |
format | Online Article Text |
id | pubmed-3516129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Libertas Academica |
record_format | MEDLINE/PubMed |
spelling | pubmed-35161292012-12-12 Effects of High Fat Feeding on Liver Gene Expression in Diabetic Goto-Kakizaki Rats Almon, Richard R. DuBois, Debra C. Sukumaran, Siddharth Wang, Xi Xue, Bai Nie, Jing Jusko, William J. Gene Regul Syst Bio Original Research Effects of high fat diet (HFD) on obesity and, subsequently, on diabetes are highly variable and modulated by genetics in both humans and rodents. In this report, we characterized the response of Goto-Kakizaki (GK) rats, a spontaneous polygenic model for lean diabetes and healthy Wistar-Kyoto (WKY) controls, to high fat feeding from weaning to 20 weeks of age. Animals fed either normal diet or HFD were sacrificed at 4, 8, 12, 16 and 20 weeks of age and a wide array of physiological measurements were made along with gene expression profiling using Affymetrix gene array chips. Mining of the microarray data identified differentially regulated genes (involved in inflammation, metabolism, transcription regulation, and signaling) in diabetic animals, as well as the response of both strains to HFD. Functional annotation suggested that HFD increased inflammatory differences between the two strains. Chronic inflammation driven by heightened innate immune response was identified to be present in GK animals regardless of diet. In addition, compensatory mechanisms by which WKY animals on HFD resisted the development of diabetes were identified, thus illustrating the complexity of diabetes disease progression. Libertas Academica 2012-11-28 /pmc/articles/PMC3516129/ /pubmed/23236253 http://dx.doi.org/10.4137/GRSB.S10371 Text en © 2012 the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited. |
spellingShingle | Original Research Almon, Richard R. DuBois, Debra C. Sukumaran, Siddharth Wang, Xi Xue, Bai Nie, Jing Jusko, William J. Effects of High Fat Feeding on Liver Gene Expression in Diabetic Goto-Kakizaki Rats |
title | Effects of High Fat Feeding on Liver Gene Expression in Diabetic Goto-Kakizaki Rats |
title_full | Effects of High Fat Feeding on Liver Gene Expression in Diabetic Goto-Kakizaki Rats |
title_fullStr | Effects of High Fat Feeding on Liver Gene Expression in Diabetic Goto-Kakizaki Rats |
title_full_unstemmed | Effects of High Fat Feeding on Liver Gene Expression in Diabetic Goto-Kakizaki Rats |
title_short | Effects of High Fat Feeding on Liver Gene Expression in Diabetic Goto-Kakizaki Rats |
title_sort | effects of high fat feeding on liver gene expression in diabetic goto-kakizaki rats |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3516129/ https://www.ncbi.nlm.nih.gov/pubmed/23236253 http://dx.doi.org/10.4137/GRSB.S10371 |
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