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Pilus Biogenesis in Lactococcus lactis: Molecular Characterization and Role in Aggregation and Biofilm Formation

The genome of Lactococcus lactis strain IL1403 harbors a putative pilus biogenesis cluster consisting of a sortase C gene flanked by 3 LPxTG protein encoding genes (yhgD, yhgE, and yhhB), called here pil. However, pili were not detected under standard growth conditions. Over-expression of the pil op...

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Autores principales: Oxaran, Virginie, Ledue-Clier, Florence, Dieye, Yakhya, Herry, Jean-Marie, Péchoux, Christine, Meylheuc, Thierry, Briandet, Romain, Juillard, Vincent, Piard, Jean-Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3516528/
https://www.ncbi.nlm.nih.gov/pubmed/23236417
http://dx.doi.org/10.1371/journal.pone.0050989
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author Oxaran, Virginie
Ledue-Clier, Florence
Dieye, Yakhya
Herry, Jean-Marie
Péchoux, Christine
Meylheuc, Thierry
Briandet, Romain
Juillard, Vincent
Piard, Jean-Christophe
author_facet Oxaran, Virginie
Ledue-Clier, Florence
Dieye, Yakhya
Herry, Jean-Marie
Péchoux, Christine
Meylheuc, Thierry
Briandet, Romain
Juillard, Vincent
Piard, Jean-Christophe
author_sort Oxaran, Virginie
collection PubMed
description The genome of Lactococcus lactis strain IL1403 harbors a putative pilus biogenesis cluster consisting of a sortase C gene flanked by 3 LPxTG protein encoding genes (yhgD, yhgE, and yhhB), called here pil. However, pili were not detected under standard growth conditions. Over-expression of the pil operon resulted in production and display of pili on the surface of lactococci. Functional analysis of the pilus biogenesis machinery indicated that the pilus shaft is formed by oligomers of the YhgE pilin, that the pilus cap is formed by the YhgD pilin and that YhhB is the basal pilin allowing the tethering of the pilus fibers to the cell wall. Oligomerization of pilin subunits was catalyzed by sortase C while anchoring of pili to the cell wall was mediated by sortase A. Piliated L. lactis cells exhibited an auto-aggregation phenotype in liquid cultures, which was attributed to the polymerization of major pilin, YhgE. The piliated lactococci formed thicker, more aerial biofilms compared to those produced by non-piliated bacteria. This phenotype was attributed to oligomers of YhgE. This study provides the first dissection of the pilus biogenesis machinery in a non-pathogenic Gram-positive bacterium. Analysis of natural lactococci isolates from clinical and vegetal environments showed pili production under standard growth conditions. The identification of functional pili in lactococci suggests that the changes they promote in aggregation and biofilm formation may be important for the natural lifestyle as well as for applications in which these bacteria are used.
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spelling pubmed-35165282012-12-12 Pilus Biogenesis in Lactococcus lactis: Molecular Characterization and Role in Aggregation and Biofilm Formation Oxaran, Virginie Ledue-Clier, Florence Dieye, Yakhya Herry, Jean-Marie Péchoux, Christine Meylheuc, Thierry Briandet, Romain Juillard, Vincent Piard, Jean-Christophe PLoS One Research Article The genome of Lactococcus lactis strain IL1403 harbors a putative pilus biogenesis cluster consisting of a sortase C gene flanked by 3 LPxTG protein encoding genes (yhgD, yhgE, and yhhB), called here pil. However, pili were not detected under standard growth conditions. Over-expression of the pil operon resulted in production and display of pili on the surface of lactococci. Functional analysis of the pilus biogenesis machinery indicated that the pilus shaft is formed by oligomers of the YhgE pilin, that the pilus cap is formed by the YhgD pilin and that YhhB is the basal pilin allowing the tethering of the pilus fibers to the cell wall. Oligomerization of pilin subunits was catalyzed by sortase C while anchoring of pili to the cell wall was mediated by sortase A. Piliated L. lactis cells exhibited an auto-aggregation phenotype in liquid cultures, which was attributed to the polymerization of major pilin, YhgE. The piliated lactococci formed thicker, more aerial biofilms compared to those produced by non-piliated bacteria. This phenotype was attributed to oligomers of YhgE. This study provides the first dissection of the pilus biogenesis machinery in a non-pathogenic Gram-positive bacterium. Analysis of natural lactococci isolates from clinical and vegetal environments showed pili production under standard growth conditions. The identification of functional pili in lactococci suggests that the changes they promote in aggregation and biofilm formation may be important for the natural lifestyle as well as for applications in which these bacteria are used. Public Library of Science 2012-12-06 /pmc/articles/PMC3516528/ /pubmed/23236417 http://dx.doi.org/10.1371/journal.pone.0050989 Text en © 2012 Oxaran et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Oxaran, Virginie
Ledue-Clier, Florence
Dieye, Yakhya
Herry, Jean-Marie
Péchoux, Christine
Meylheuc, Thierry
Briandet, Romain
Juillard, Vincent
Piard, Jean-Christophe
Pilus Biogenesis in Lactococcus lactis: Molecular Characterization and Role in Aggregation and Biofilm Formation
title Pilus Biogenesis in Lactococcus lactis: Molecular Characterization and Role in Aggregation and Biofilm Formation
title_full Pilus Biogenesis in Lactococcus lactis: Molecular Characterization and Role in Aggregation and Biofilm Formation
title_fullStr Pilus Biogenesis in Lactococcus lactis: Molecular Characterization and Role in Aggregation and Biofilm Formation
title_full_unstemmed Pilus Biogenesis in Lactococcus lactis: Molecular Characterization and Role in Aggregation and Biofilm Formation
title_short Pilus Biogenesis in Lactococcus lactis: Molecular Characterization and Role in Aggregation and Biofilm Formation
title_sort pilus biogenesis in lactococcus lactis: molecular characterization and role in aggregation and biofilm formation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3516528/
https://www.ncbi.nlm.nih.gov/pubmed/23236417
http://dx.doi.org/10.1371/journal.pone.0050989
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