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Tamm-Horsfall protein facilitates catheter associated urinary tract infection

BACKGROUND: Urinary catheters are associated, commonly with bacteriuria and frequently with urinary tract infection. Tamm-Horsfall Protein (THP) is urine's most abundant protein and is known to bind to uropathogenic bacteria. The role of THP in the pathogenesis of catheter associated urinary tr...

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Autores principales: Raffi, Hajamohideen S, Bates, James M, Flournoy, Dayl J, Kumar, Satish
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517338/
https://www.ncbi.nlm.nih.gov/pubmed/23009031
http://dx.doi.org/10.1186/1756-0500-5-532
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author Raffi, Hajamohideen S
Bates, James M
Flournoy, Dayl J
Kumar, Satish
author_facet Raffi, Hajamohideen S
Bates, James M
Flournoy, Dayl J
Kumar, Satish
author_sort Raffi, Hajamohideen S
collection PubMed
description BACKGROUND: Urinary catheters are associated, commonly with bacteriuria and frequently with urinary tract infection. Tamm-Horsfall Protein (THP) is urine's most abundant protein and is known to bind to uropathogenic bacteria. The role of THP in the pathogenesis of catheter associated urinary tract infection (CAUTI) is not clear. We examined the role of THP in facilitating bacterial binding to urinary catheters in vivo and in vitro. FINDINGS: Twenty one urinary catheters were obtained from 20 hospitalized patients. THP was eluted from the catheter surface and catheter segments were cultured. Additional studies were performed in vitro on unused silicone and latex catheters to determine the binding of THP, and the effect of THP on the binding of Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), to the catheter surface. On catheters obtained from patients, the THP deposition was significantly more on culture positive catheters than on culture negative catheters. In the in vitro studies, THP bound to both silicone and latex catheters, and THP enhanced the adherence of E. coli and P. aeruginosa to both types of catheters. CONCLUSION: THP binds to urinary catheters and facilitates the binding of uropathogenic bacteria to catheters.
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spelling pubmed-35173382012-12-08 Tamm-Horsfall protein facilitates catheter associated urinary tract infection Raffi, Hajamohideen S Bates, James M Flournoy, Dayl J Kumar, Satish BMC Res Notes Short Report BACKGROUND: Urinary catheters are associated, commonly with bacteriuria and frequently with urinary tract infection. Tamm-Horsfall Protein (THP) is urine's most abundant protein and is known to bind to uropathogenic bacteria. The role of THP in the pathogenesis of catheter associated urinary tract infection (CAUTI) is not clear. We examined the role of THP in facilitating bacterial binding to urinary catheters in vivo and in vitro. FINDINGS: Twenty one urinary catheters were obtained from 20 hospitalized patients. THP was eluted from the catheter surface and catheter segments were cultured. Additional studies were performed in vitro on unused silicone and latex catheters to determine the binding of THP, and the effect of THP on the binding of Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), to the catheter surface. On catheters obtained from patients, the THP deposition was significantly more on culture positive catheters than on culture negative catheters. In the in vitro studies, THP bound to both silicone and latex catheters, and THP enhanced the adherence of E. coli and P. aeruginosa to both types of catheters. CONCLUSION: THP binds to urinary catheters and facilitates the binding of uropathogenic bacteria to catheters. BioMed Central 2012-09-26 /pmc/articles/PMC3517338/ /pubmed/23009031 http://dx.doi.org/10.1186/1756-0500-5-532 Text en Copyright ©2012 Raffi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Raffi, Hajamohideen S
Bates, James M
Flournoy, Dayl J
Kumar, Satish
Tamm-Horsfall protein facilitates catheter associated urinary tract infection
title Tamm-Horsfall protein facilitates catheter associated urinary tract infection
title_full Tamm-Horsfall protein facilitates catheter associated urinary tract infection
title_fullStr Tamm-Horsfall protein facilitates catheter associated urinary tract infection
title_full_unstemmed Tamm-Horsfall protein facilitates catheter associated urinary tract infection
title_short Tamm-Horsfall protein facilitates catheter associated urinary tract infection
title_sort tamm-horsfall protein facilitates catheter associated urinary tract infection
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517338/
https://www.ncbi.nlm.nih.gov/pubmed/23009031
http://dx.doi.org/10.1186/1756-0500-5-532
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