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Tamm-Horsfall protein facilitates catheter associated urinary tract infection
BACKGROUND: Urinary catheters are associated, commonly with bacteriuria and frequently with urinary tract infection. Tamm-Horsfall Protein (THP) is urine's most abundant protein and is known to bind to uropathogenic bacteria. The role of THP in the pathogenesis of catheter associated urinary tr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517338/ https://www.ncbi.nlm.nih.gov/pubmed/23009031 http://dx.doi.org/10.1186/1756-0500-5-532 |
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author | Raffi, Hajamohideen S Bates, James M Flournoy, Dayl J Kumar, Satish |
author_facet | Raffi, Hajamohideen S Bates, James M Flournoy, Dayl J Kumar, Satish |
author_sort | Raffi, Hajamohideen S |
collection | PubMed |
description | BACKGROUND: Urinary catheters are associated, commonly with bacteriuria and frequently with urinary tract infection. Tamm-Horsfall Protein (THP) is urine's most abundant protein and is known to bind to uropathogenic bacteria. The role of THP in the pathogenesis of catheter associated urinary tract infection (CAUTI) is not clear. We examined the role of THP in facilitating bacterial binding to urinary catheters in vivo and in vitro. FINDINGS: Twenty one urinary catheters were obtained from 20 hospitalized patients. THP was eluted from the catheter surface and catheter segments were cultured. Additional studies were performed in vitro on unused silicone and latex catheters to determine the binding of THP, and the effect of THP on the binding of Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), to the catheter surface. On catheters obtained from patients, the THP deposition was significantly more on culture positive catheters than on culture negative catheters. In the in vitro studies, THP bound to both silicone and latex catheters, and THP enhanced the adherence of E. coli and P. aeruginosa to both types of catheters. CONCLUSION: THP binds to urinary catheters and facilitates the binding of uropathogenic bacteria to catheters. |
format | Online Article Text |
id | pubmed-3517338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35173382012-12-08 Tamm-Horsfall protein facilitates catheter associated urinary tract infection Raffi, Hajamohideen S Bates, James M Flournoy, Dayl J Kumar, Satish BMC Res Notes Short Report BACKGROUND: Urinary catheters are associated, commonly with bacteriuria and frequently with urinary tract infection. Tamm-Horsfall Protein (THP) is urine's most abundant protein and is known to bind to uropathogenic bacteria. The role of THP in the pathogenesis of catheter associated urinary tract infection (CAUTI) is not clear. We examined the role of THP in facilitating bacterial binding to urinary catheters in vivo and in vitro. FINDINGS: Twenty one urinary catheters were obtained from 20 hospitalized patients. THP was eluted from the catheter surface and catheter segments were cultured. Additional studies were performed in vitro on unused silicone and latex catheters to determine the binding of THP, and the effect of THP on the binding of Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), to the catheter surface. On catheters obtained from patients, the THP deposition was significantly more on culture positive catheters than on culture negative catheters. In the in vitro studies, THP bound to both silicone and latex catheters, and THP enhanced the adherence of E. coli and P. aeruginosa to both types of catheters. CONCLUSION: THP binds to urinary catheters and facilitates the binding of uropathogenic bacteria to catheters. BioMed Central 2012-09-26 /pmc/articles/PMC3517338/ /pubmed/23009031 http://dx.doi.org/10.1186/1756-0500-5-532 Text en Copyright ©2012 Raffi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Raffi, Hajamohideen S Bates, James M Flournoy, Dayl J Kumar, Satish Tamm-Horsfall protein facilitates catheter associated urinary tract infection |
title | Tamm-Horsfall protein facilitates catheter associated urinary tract infection |
title_full | Tamm-Horsfall protein facilitates catheter associated urinary tract infection |
title_fullStr | Tamm-Horsfall protein facilitates catheter associated urinary tract infection |
title_full_unstemmed | Tamm-Horsfall protein facilitates catheter associated urinary tract infection |
title_short | Tamm-Horsfall protein facilitates catheter associated urinary tract infection |
title_sort | tamm-horsfall protein facilitates catheter associated urinary tract infection |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517338/ https://www.ncbi.nlm.nih.gov/pubmed/23009031 http://dx.doi.org/10.1186/1756-0500-5-532 |
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