Cargando…

Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species

Bornavirus, a non-segmented, negative-strand RNA viruses, is currently classified into several genetically distinct genotypes, such as Borna disease virus (BDV) and avian bornaviruses (ABVs). Recent studies revealed that bornavirus genotypes show unique sequence variability in the putative 5′ untran...

Descripción completa

Detalles Bibliográficos
Autores principales: Fujino, Kan, Horie, Masayuki, Honda, Tomoyuki, Nakamura, Shoko, Matsumoto, Yusuke, Francischetti, Ivo M. B., Tomonaga, Keizo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517445/
https://www.ncbi.nlm.nih.gov/pubmed/23236446
http://dx.doi.org/10.1371/journal.pone.0051161
_version_ 1782252412856172544
author Fujino, Kan
Horie, Masayuki
Honda, Tomoyuki
Nakamura, Shoko
Matsumoto, Yusuke
Francischetti, Ivo M. B.
Tomonaga, Keizo
author_facet Fujino, Kan
Horie, Masayuki
Honda, Tomoyuki
Nakamura, Shoko
Matsumoto, Yusuke
Francischetti, Ivo M. B.
Tomonaga, Keizo
author_sort Fujino, Kan
collection PubMed
description Bornavirus, a non-segmented, negative-strand RNA viruses, is currently classified into several genetically distinct genotypes, such as Borna disease virus (BDV) and avian bornaviruses (ABVs). Recent studies revealed that bornavirus genotypes show unique sequence variability in the putative 5′ untranslated region (5′ UTR) of X/P mRNA, a bicistronic mRNA for the X protein and phosphoprotein (P). In this study, to understand the evolutionary relationship among the bornavirus genotypes, we investigated the functional interaction between the X and P proteins of four bornavirus genotypes, BDV, ABV genotype 4 and 5 and reptile bornavirus (RBV), the putative 5′ UTRs of which exhibit variation in the length. Immunofluorescence and immunoprecipitation analyses using mammalian and avian cell lines revealed that the X proteins of bornaviruses conserve the ability to facilitate the export of P from the nucleus to the cytoplasm via interaction with P. Furthermore, we showed that inter-genotypic interactions may occur between X and P among the genotypes, except for X of RBV. In addition, a BDV minireplicon assay demonstrated that the X and P proteins of ABVs, but not RBV, can affect the polymerase activity of BDV. This study demonstrates that bornaviruses may have conserved the fundamental function of a regulatory protein during their evolution, whereas RBV has evolved distinctly from the other bornavirus genotypes.
format Online
Article
Text
id pubmed-3517445
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-35174452012-12-12 Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species Fujino, Kan Horie, Masayuki Honda, Tomoyuki Nakamura, Shoko Matsumoto, Yusuke Francischetti, Ivo M. B. Tomonaga, Keizo PLoS One Research Article Bornavirus, a non-segmented, negative-strand RNA viruses, is currently classified into several genetically distinct genotypes, such as Borna disease virus (BDV) and avian bornaviruses (ABVs). Recent studies revealed that bornavirus genotypes show unique sequence variability in the putative 5′ untranslated region (5′ UTR) of X/P mRNA, a bicistronic mRNA for the X protein and phosphoprotein (P). In this study, to understand the evolutionary relationship among the bornavirus genotypes, we investigated the functional interaction between the X and P proteins of four bornavirus genotypes, BDV, ABV genotype 4 and 5 and reptile bornavirus (RBV), the putative 5′ UTRs of which exhibit variation in the length. Immunofluorescence and immunoprecipitation analyses using mammalian and avian cell lines revealed that the X proteins of bornaviruses conserve the ability to facilitate the export of P from the nucleus to the cytoplasm via interaction with P. Furthermore, we showed that inter-genotypic interactions may occur between X and P among the genotypes, except for X of RBV. In addition, a BDV minireplicon assay demonstrated that the X and P proteins of ABVs, but not RBV, can affect the polymerase activity of BDV. This study demonstrates that bornaviruses may have conserved the fundamental function of a regulatory protein during their evolution, whereas RBV has evolved distinctly from the other bornavirus genotypes. Public Library of Science 2012-12-07 /pmc/articles/PMC3517445/ /pubmed/23236446 http://dx.doi.org/10.1371/journal.pone.0051161 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Fujino, Kan
Horie, Masayuki
Honda, Tomoyuki
Nakamura, Shoko
Matsumoto, Yusuke
Francischetti, Ivo M. B.
Tomonaga, Keizo
Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species
title Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species
title_full Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species
title_fullStr Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species
title_full_unstemmed Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species
title_short Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species
title_sort evolutionarily conserved interaction between the phosphoproteins and x proteins of bornaviruses from different vertebrate species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517445/
https://www.ncbi.nlm.nih.gov/pubmed/23236446
http://dx.doi.org/10.1371/journal.pone.0051161
work_keys_str_mv AT fujinokan evolutionarilyconservedinteractionbetweenthephosphoproteinsandxproteinsofbornavirusesfromdifferentvertebratespecies
AT horiemasayuki evolutionarilyconservedinteractionbetweenthephosphoproteinsandxproteinsofbornavirusesfromdifferentvertebratespecies
AT hondatomoyuki evolutionarilyconservedinteractionbetweenthephosphoproteinsandxproteinsofbornavirusesfromdifferentvertebratespecies
AT nakamurashoko evolutionarilyconservedinteractionbetweenthephosphoproteinsandxproteinsofbornavirusesfromdifferentvertebratespecies
AT matsumotoyusuke evolutionarilyconservedinteractionbetweenthephosphoproteinsandxproteinsofbornavirusesfromdifferentvertebratespecies
AT francischettiivomb evolutionarilyconservedinteractionbetweenthephosphoproteinsandxproteinsofbornavirusesfromdifferentvertebratespecies
AT tomonagakeizo evolutionarilyconservedinteractionbetweenthephosphoproteinsandxproteinsofbornavirusesfromdifferentvertebratespecies