Cargando…
Low BRMS1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival
BACKGROUND: Breast cancer metastasis suppressor 1 (BRMS1) is a metastasis suppressor gene. This study aimed to investigate the impact of BRMS1 on metastasis in nasopharyngeal carcinoma (NPC) and to evaluate the prognostic significance of BRMS1 in NPC patients. METHODS: BRMS1 expression was examined...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517767/ https://www.ncbi.nlm.nih.gov/pubmed/22931099 http://dx.doi.org/10.1186/1471-2407-12-376 |
_version_ | 1782252471098277888 |
---|---|
author | He, Qing-Mei Li, Wen-Fei Huang, Bi-Jun Sun, Ying Tang, Ling-Long Chen, Mo Jiang, Ning Chen, Lei Yun, Jing-Ping Zeng, Jing Guo, Ying Wang, Hui-Yun Ma, Jun |
author_facet | He, Qing-Mei Li, Wen-Fei Huang, Bi-Jun Sun, Ying Tang, Ling-Long Chen, Mo Jiang, Ning Chen, Lei Yun, Jing-Ping Zeng, Jing Guo, Ying Wang, Hui-Yun Ma, Jun |
author_sort | He, Qing-Mei |
collection | PubMed |
description | BACKGROUND: Breast cancer metastasis suppressor 1 (BRMS1) is a metastasis suppressor gene. This study aimed to investigate the impact of BRMS1 on metastasis in nasopharyngeal carcinoma (NPC) and to evaluate the prognostic significance of BRMS1 in NPC patients. METHODS: BRMS1 expression was examined in NPC cell lines using quantitative reverse transcription-polymerase chain reaction and Western blotting. NPC cells stably expressing BRMS1 were used to perform wound healing and invasion assays in vitro and a murine xenograft assay in vivo. Immunohistochemical staining was performed in 274 paraffin-embedded NPC specimens divided into a training set (n = 120) and a testing set (n = 154). RESULTS: BRMS1 expression was down-regulated in NPC cell lines. Overexpression of BRMS1 significantly reversed the metastatic phenotype of NPC cells in vitro and in vivo. Importantly, low BRMS1 expression was associated with poor distant metastasis-free survival (DMFS, P < 0.001) and poor overall survival (OS, P < 0.001) in the training set; these results were validated in the testing set and overall patient population. Cox regression analysis demonstrated that low BRMS1 expression was an independent prognostic factor for DMFS and OS in NPC. CONCLUSIONS: Low expression of the metastasis suppressor BRMS1 may be an independent prognostic factor for poor prognosis in NPC patients. |
format | Online Article Text |
id | pubmed-3517767 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35177672012-12-09 Low BRMS1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival He, Qing-Mei Li, Wen-Fei Huang, Bi-Jun Sun, Ying Tang, Ling-Long Chen, Mo Jiang, Ning Chen, Lei Yun, Jing-Ping Zeng, Jing Guo, Ying Wang, Hui-Yun Ma, Jun BMC Cancer Research Article BACKGROUND: Breast cancer metastasis suppressor 1 (BRMS1) is a metastasis suppressor gene. This study aimed to investigate the impact of BRMS1 on metastasis in nasopharyngeal carcinoma (NPC) and to evaluate the prognostic significance of BRMS1 in NPC patients. METHODS: BRMS1 expression was examined in NPC cell lines using quantitative reverse transcription-polymerase chain reaction and Western blotting. NPC cells stably expressing BRMS1 were used to perform wound healing and invasion assays in vitro and a murine xenograft assay in vivo. Immunohistochemical staining was performed in 274 paraffin-embedded NPC specimens divided into a training set (n = 120) and a testing set (n = 154). RESULTS: BRMS1 expression was down-regulated in NPC cell lines. Overexpression of BRMS1 significantly reversed the metastatic phenotype of NPC cells in vitro and in vivo. Importantly, low BRMS1 expression was associated with poor distant metastasis-free survival (DMFS, P < 0.001) and poor overall survival (OS, P < 0.001) in the training set; these results were validated in the testing set and overall patient population. Cox regression analysis demonstrated that low BRMS1 expression was an independent prognostic factor for DMFS and OS in NPC. CONCLUSIONS: Low expression of the metastasis suppressor BRMS1 may be an independent prognostic factor for poor prognosis in NPC patients. BioMed Central 2012-08-29 /pmc/articles/PMC3517767/ /pubmed/22931099 http://dx.doi.org/10.1186/1471-2407-12-376 Text en Copyright ©2012 Cui et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article He, Qing-Mei Li, Wen-Fei Huang, Bi-Jun Sun, Ying Tang, Ling-Long Chen, Mo Jiang, Ning Chen, Lei Yun, Jing-Ping Zeng, Jing Guo, Ying Wang, Hui-Yun Ma, Jun Low BRMS1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival |
title | Low BRMS1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival |
title_full | Low BRMS1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival |
title_fullStr | Low BRMS1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival |
title_full_unstemmed | Low BRMS1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival |
title_short | Low BRMS1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival |
title_sort | low brms1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517767/ https://www.ncbi.nlm.nih.gov/pubmed/22931099 http://dx.doi.org/10.1186/1471-2407-12-376 |
work_keys_str_mv | AT heqingmei lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival AT liwenfei lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival AT huangbijun lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival AT sunying lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival AT tanglinglong lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival AT chenmo lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival AT jiangning lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival AT chenlei lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival AT yunjingping lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival AT zengjing lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival AT guoying lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival AT wanghuiyun lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival AT majun lowbrms1expressionpromotesnasopharyngealcarcinomametastasisinvitroandinvivoandisassociatedwithpoorpatientsurvival |