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Temporal expression analysis of angiogenesis-related genes in brain development
BACKGROUND: The current knowledge on molecular pathogenesis of cerebral vascular malformations (CVM), which are believed to arise during development, is very limited. To unravel the molecular mechanisms involved in CVMs, a detailed understanding of the brain vascular development at molecular level i...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517775/ https://www.ncbi.nlm.nih.gov/pubmed/23020941 http://dx.doi.org/10.1186/2045-824X-4-16 |
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author | Özkan, Abdulkadir Biçer, Atilla Avşar, Timuçin Şeker, Askin Toktaş, Zafer Orkun Bozkurt, Süheyla Uyar Başak, Ayse Nazli Kılıç, Türker |
author_facet | Özkan, Abdulkadir Biçer, Atilla Avşar, Timuçin Şeker, Askin Toktaş, Zafer Orkun Bozkurt, Süheyla Uyar Başak, Ayse Nazli Kılıç, Türker |
author_sort | Özkan, Abdulkadir |
collection | PubMed |
description | BACKGROUND: The current knowledge on molecular pathogenesis of cerebral vascular malformations (CVM), which are believed to arise during development, is very limited. To unravel the molecular mechanisms involved in CVMs, a detailed understanding of the brain vascular development at molecular level is crucial. In this study, we aimed to explore the temporal and comparative expression profile of angiogenesis-related genes in the establishment of brain vasculature. METHODS: Expression of a total of 113 angiogenesis-related genes during murine brain development has been analyzed using low-density array systems designed for angiogenesis-related genes. Bai1 (brain specific angiogenesis inhibitor-1), a recently identified novel anti-angiogenic gene, has been selected for further characterization. RESULTS: We found that 62 out of 113 analyzed genes have expression in brain development at varying levels. Nineteen of these were differentially expressed between embryonic and postnatal stages (>1.5 fold). Bai1 is strongly expressed on growing blood vessels of cerebral cortex and hippocampus, partially expressed in the lateral regions of striatum, but mostly absent on the thalamus. CONCLUSION: By showing the comparative expression analysis of angiogenesis-related genes throughout brain development, the data presented here will be a crucial addition to further functional studies on cerebrovascular research. |
format | Online Article Text |
id | pubmed-3517775 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35177752012-12-09 Temporal expression analysis of angiogenesis-related genes in brain development Özkan, Abdulkadir Biçer, Atilla Avşar, Timuçin Şeker, Askin Toktaş, Zafer Orkun Bozkurt, Süheyla Uyar Başak, Ayse Nazli Kılıç, Türker Vasc Cell Research BACKGROUND: The current knowledge on molecular pathogenesis of cerebral vascular malformations (CVM), which are believed to arise during development, is very limited. To unravel the molecular mechanisms involved in CVMs, a detailed understanding of the brain vascular development at molecular level is crucial. In this study, we aimed to explore the temporal and comparative expression profile of angiogenesis-related genes in the establishment of brain vasculature. METHODS: Expression of a total of 113 angiogenesis-related genes during murine brain development has been analyzed using low-density array systems designed for angiogenesis-related genes. Bai1 (brain specific angiogenesis inhibitor-1), a recently identified novel anti-angiogenic gene, has been selected for further characterization. RESULTS: We found that 62 out of 113 analyzed genes have expression in brain development at varying levels. Nineteen of these were differentially expressed between embryonic and postnatal stages (>1.5 fold). Bai1 is strongly expressed on growing blood vessels of cerebral cortex and hippocampus, partially expressed in the lateral regions of striatum, but mostly absent on the thalamus. CONCLUSION: By showing the comparative expression analysis of angiogenesis-related genes throughout brain development, the data presented here will be a crucial addition to further functional studies on cerebrovascular research. BioMed Central 2012-10-01 /pmc/articles/PMC3517775/ /pubmed/23020941 http://dx.doi.org/10.1186/2045-824X-4-16 Text en Copyright ©2012 Özkan et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Özkan, Abdulkadir Biçer, Atilla Avşar, Timuçin Şeker, Askin Toktaş, Zafer Orkun Bozkurt, Süheyla Uyar Başak, Ayse Nazli Kılıç, Türker Temporal expression analysis of angiogenesis-related genes in brain development |
title | Temporal expression analysis of angiogenesis-related genes in brain development |
title_full | Temporal expression analysis of angiogenesis-related genes in brain development |
title_fullStr | Temporal expression analysis of angiogenesis-related genes in brain development |
title_full_unstemmed | Temporal expression analysis of angiogenesis-related genes in brain development |
title_short | Temporal expression analysis of angiogenesis-related genes in brain development |
title_sort | temporal expression analysis of angiogenesis-related genes in brain development |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517775/ https://www.ncbi.nlm.nih.gov/pubmed/23020941 http://dx.doi.org/10.1186/2045-824X-4-16 |
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