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Temporal expression analysis of angiogenesis-related genes in brain development

BACKGROUND: The current knowledge on molecular pathogenesis of cerebral vascular malformations (CVM), which are believed to arise during development, is very limited. To unravel the molecular mechanisms involved in CVMs, a detailed understanding of the brain vascular development at molecular level i...

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Autores principales: Özkan, Abdulkadir, Biçer, Atilla, Avşar, Timuçin, Şeker, Askin, Toktaş, Zafer Orkun, Bozkurt, Süheyla Uyar, Başak, Ayse Nazli, Kılıç, Türker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517775/
https://www.ncbi.nlm.nih.gov/pubmed/23020941
http://dx.doi.org/10.1186/2045-824X-4-16
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author Özkan, Abdulkadir
Biçer, Atilla
Avşar, Timuçin
Şeker, Askin
Toktaş, Zafer Orkun
Bozkurt, Süheyla Uyar
Başak, Ayse Nazli
Kılıç, Türker
author_facet Özkan, Abdulkadir
Biçer, Atilla
Avşar, Timuçin
Şeker, Askin
Toktaş, Zafer Orkun
Bozkurt, Süheyla Uyar
Başak, Ayse Nazli
Kılıç, Türker
author_sort Özkan, Abdulkadir
collection PubMed
description BACKGROUND: The current knowledge on molecular pathogenesis of cerebral vascular malformations (CVM), which are believed to arise during development, is very limited. To unravel the molecular mechanisms involved in CVMs, a detailed understanding of the brain vascular development at molecular level is crucial. In this study, we aimed to explore the temporal and comparative expression profile of angiogenesis-related genes in the establishment of brain vasculature. METHODS: Expression of a total of 113 angiogenesis-related genes during murine brain development has been analyzed using low-density array systems designed for angiogenesis-related genes. Bai1 (brain specific angiogenesis inhibitor-1), a recently identified novel anti-angiogenic gene, has been selected for further characterization. RESULTS: We found that 62 out of 113 analyzed genes have expression in brain development at varying levels. Nineteen of these were differentially expressed between embryonic and postnatal stages (>1.5 fold). Bai1 is strongly expressed on growing blood vessels of cerebral cortex and hippocampus, partially expressed in the lateral regions of striatum, but mostly absent on the thalamus. CONCLUSION: By showing the comparative expression analysis of angiogenesis-related genes throughout brain development, the data presented here will be a crucial addition to further functional studies on cerebrovascular research.
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spelling pubmed-35177752012-12-09 Temporal expression analysis of angiogenesis-related genes in brain development Özkan, Abdulkadir Biçer, Atilla Avşar, Timuçin Şeker, Askin Toktaş, Zafer Orkun Bozkurt, Süheyla Uyar Başak, Ayse Nazli Kılıç, Türker Vasc Cell Research BACKGROUND: The current knowledge on molecular pathogenesis of cerebral vascular malformations (CVM), which are believed to arise during development, is very limited. To unravel the molecular mechanisms involved in CVMs, a detailed understanding of the brain vascular development at molecular level is crucial. In this study, we aimed to explore the temporal and comparative expression profile of angiogenesis-related genes in the establishment of brain vasculature. METHODS: Expression of a total of 113 angiogenesis-related genes during murine brain development has been analyzed using low-density array systems designed for angiogenesis-related genes. Bai1 (brain specific angiogenesis inhibitor-1), a recently identified novel anti-angiogenic gene, has been selected for further characterization. RESULTS: We found that 62 out of 113 analyzed genes have expression in brain development at varying levels. Nineteen of these were differentially expressed between embryonic and postnatal stages (>1.5 fold). Bai1 is strongly expressed on growing blood vessels of cerebral cortex and hippocampus, partially expressed in the lateral regions of striatum, but mostly absent on the thalamus. CONCLUSION: By showing the comparative expression analysis of angiogenesis-related genes throughout brain development, the data presented here will be a crucial addition to further functional studies on cerebrovascular research. BioMed Central 2012-10-01 /pmc/articles/PMC3517775/ /pubmed/23020941 http://dx.doi.org/10.1186/2045-824X-4-16 Text en Copyright ©2012 Özkan et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Özkan, Abdulkadir
Biçer, Atilla
Avşar, Timuçin
Şeker, Askin
Toktaş, Zafer Orkun
Bozkurt, Süheyla Uyar
Başak, Ayse Nazli
Kılıç, Türker
Temporal expression analysis of angiogenesis-related genes in brain development
title Temporal expression analysis of angiogenesis-related genes in brain development
title_full Temporal expression analysis of angiogenesis-related genes in brain development
title_fullStr Temporal expression analysis of angiogenesis-related genes in brain development
title_full_unstemmed Temporal expression analysis of angiogenesis-related genes in brain development
title_short Temporal expression analysis of angiogenesis-related genes in brain development
title_sort temporal expression analysis of angiogenesis-related genes in brain development
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517775/
https://www.ncbi.nlm.nih.gov/pubmed/23020941
http://dx.doi.org/10.1186/2045-824X-4-16
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