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Inhibition of Vitamin D Receptor Translocation by Cigarette Smoking Extracts

BACKGROUND: Vitamin D can translocate a vitamin D receptor (VDR) from the nucleus to the cell membranes. The meaning of this translocation is not elucidated in terms of a role in pathogenesis of chronic obstructive pulmonary disease (COPD) till now. VDR deficient mice are prone to develop emphysema,...

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Autores principales: Uh, Soo-Taek, Koo, So-My, Kim, Yang Ki, Kim, Ki Up, Park, Sung Woo, Jang, An Soo, Kim, Do Jin, Kim, Yong Hoon, Park, Choon Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Tuberculosis and Respiratory Diseases 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517944/
https://www.ncbi.nlm.nih.gov/pubmed/23236317
http://dx.doi.org/10.4046/trd.2012.73.5.258
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author Uh, Soo-Taek
Koo, So-My
Kim, Yang Ki
Kim, Ki Up
Park, Sung Woo
Jang, An Soo
Kim, Do Jin
Kim, Yong Hoon
Park, Choon Sik
author_facet Uh, Soo-Taek
Koo, So-My
Kim, Yang Ki
Kim, Ki Up
Park, Sung Woo
Jang, An Soo
Kim, Do Jin
Kim, Yong Hoon
Park, Choon Sik
author_sort Uh, Soo-Taek
collection PubMed
description BACKGROUND: Vitamin D can translocate a vitamin D receptor (VDR) from the nucleus to the cell membranes. The meaning of this translocation is not elucidated in terms of a role in pathogenesis of chronic obstructive pulmonary disease (COPD) till now. VDR deficient mice are prone to develop emphysema, suggesting that abnormal function of VDR might influence a generation of COPD. The blood levels of vitamin D have known to be well correlated with that of lung function in patients with COPD, and smoking is the most important risk factor in development of COPD. This study was performed to investigate whether cigarette smoke extracts (CSE) can inhibit the translocation of VDR and whether mitogen activated protein kinases (MAPKs) are involved in this inhibition. METHODS: Human alveolar basal epithelial cell line (A549) was used in this study. 1,25-(OH(2))D(3) and/or MAPKs inhibitors and antioxidants were pre-incubated before stimulation with 10% CSE, and then nucleus and microsomal proteins were extracted for a Western blot of VDR. RESULTS: Five minutes treatment of 1,25-(OH(2))D(3) induced translocation of VDR from nucleus to microsomes by a dose-dependent manner. CSE inhibited 1,25-(OH(2))D(3)-induced translocation of VDR in both concentrations of 10% and 20%. All MAPKs inhibitors did not suppress the inhibitory effects of CSE on the 1,25-(OH(2))D(3)-induced translocation of VDR. Quercetin suppressed the inhibitory effects of CSE on the 1,25-(OH(2))D(3)-induced translocation of VDR, but not in n-acetylcysteine. CONCLUSION: CSE has an ability to inhibit vitamin D-induced VDR translocation, but MAPKs are not involved in this inhibition.
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spelling pubmed-35179442012-12-12 Inhibition of Vitamin D Receptor Translocation by Cigarette Smoking Extracts Uh, Soo-Taek Koo, So-My Kim, Yang Ki Kim, Ki Up Park, Sung Woo Jang, An Soo Kim, Do Jin Kim, Yong Hoon Park, Choon Sik Tuberc Respir Dis (Seoul) Original Article BACKGROUND: Vitamin D can translocate a vitamin D receptor (VDR) from the nucleus to the cell membranes. The meaning of this translocation is not elucidated in terms of a role in pathogenesis of chronic obstructive pulmonary disease (COPD) till now. VDR deficient mice are prone to develop emphysema, suggesting that abnormal function of VDR might influence a generation of COPD. The blood levels of vitamin D have known to be well correlated with that of lung function in patients with COPD, and smoking is the most important risk factor in development of COPD. This study was performed to investigate whether cigarette smoke extracts (CSE) can inhibit the translocation of VDR and whether mitogen activated protein kinases (MAPKs) are involved in this inhibition. METHODS: Human alveolar basal epithelial cell line (A549) was used in this study. 1,25-(OH(2))D(3) and/or MAPKs inhibitors and antioxidants were pre-incubated before stimulation with 10% CSE, and then nucleus and microsomal proteins were extracted for a Western blot of VDR. RESULTS: Five minutes treatment of 1,25-(OH(2))D(3) induced translocation of VDR from nucleus to microsomes by a dose-dependent manner. CSE inhibited 1,25-(OH(2))D(3)-induced translocation of VDR in both concentrations of 10% and 20%. All MAPKs inhibitors did not suppress the inhibitory effects of CSE on the 1,25-(OH(2))D(3)-induced translocation of VDR. Quercetin suppressed the inhibitory effects of CSE on the 1,25-(OH(2))D(3)-induced translocation of VDR, but not in n-acetylcysteine. CONCLUSION: CSE has an ability to inhibit vitamin D-induced VDR translocation, but MAPKs are not involved in this inhibition. The Korean Academy of Tuberculosis and Respiratory Diseases 2012-11 2012-11-30 /pmc/articles/PMC3517944/ /pubmed/23236317 http://dx.doi.org/10.4046/trd.2012.73.5.258 Text en Copyright©2012. The Korean Academy of Tuberculosis and Respiratory Diseases. All rights reserved. http://creativecommons.org/licenses/by-nc/3.0/ It is identical to the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/)
spellingShingle Original Article
Uh, Soo-Taek
Koo, So-My
Kim, Yang Ki
Kim, Ki Up
Park, Sung Woo
Jang, An Soo
Kim, Do Jin
Kim, Yong Hoon
Park, Choon Sik
Inhibition of Vitamin D Receptor Translocation by Cigarette Smoking Extracts
title Inhibition of Vitamin D Receptor Translocation by Cigarette Smoking Extracts
title_full Inhibition of Vitamin D Receptor Translocation by Cigarette Smoking Extracts
title_fullStr Inhibition of Vitamin D Receptor Translocation by Cigarette Smoking Extracts
title_full_unstemmed Inhibition of Vitamin D Receptor Translocation by Cigarette Smoking Extracts
title_short Inhibition of Vitamin D Receptor Translocation by Cigarette Smoking Extracts
title_sort inhibition of vitamin d receptor translocation by cigarette smoking extracts
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517944/
https://www.ncbi.nlm.nih.gov/pubmed/23236317
http://dx.doi.org/10.4046/trd.2012.73.5.258
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