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Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp. strain YSCB
Spinae are tubular surface appendages broadly found in Gram-negative bacteria. Little is known about their architecture, function or origin. Here, we report structural characterization of the spinae from marine bacteria Roseobacter sp. YSCB. Electron cryo-tomography revealed that a single filament w...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517982/ https://www.ncbi.nlm.nih.gov/pubmed/23230515 http://dx.doi.org/10.1038/srep00950 |
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author | Bernadac, A. Wu, L.-F. Santini, C.-L. Vidaud, C. Sturgis, J. N. Menguy, N. Bergam, P. Nicoletti, C. Xiao, T. |
author_facet | Bernadac, A. Wu, L.-F. Santini, C.-L. Vidaud, C. Sturgis, J. N. Menguy, N. Bergam, P. Nicoletti, C. Xiao, T. |
author_sort | Bernadac, A. |
collection | PubMed |
description | Spinae are tubular surface appendages broadly found in Gram-negative bacteria. Little is known about their architecture, function or origin. Here, we report structural characterization of the spinae from marine bacteria Roseobacter sp. YSCB. Electron cryo-tomography revealed that a single filament winds into a hollow flared base with progressive change to a cylinder. Proteinase K unwound the spinae into proteolysis-resistant filaments. Thermal treatment ripped the spinae into ribbons that were melted with prolonged heating. Circular dichroism spectroscopy revealed a dominant beta-structure of the spinae. Differential scanning calorimetry analyses showed three endothermic transformations at 50–85°C, 98°C and 123°C, respectively. The heating almost completely disintegrated the spinae, abolished the 98°C transition and destroyed the beta-structure. Infrared spectroscopy identified the amide I spectrum maximum at a position similar to that of amyloid fibrils. Therefore, the spinae distinguish from other bacterial appendages, e.g. flagella and stalks, in both the structure and mechanism of assembly. |
format | Online Article Text |
id | pubmed-3517982 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-35179822012-12-10 Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp. strain YSCB Bernadac, A. Wu, L.-F. Santini, C.-L. Vidaud, C. Sturgis, J. N. Menguy, N. Bergam, P. Nicoletti, C. Xiao, T. Sci Rep Article Spinae are tubular surface appendages broadly found in Gram-negative bacteria. Little is known about their architecture, function or origin. Here, we report structural characterization of the spinae from marine bacteria Roseobacter sp. YSCB. Electron cryo-tomography revealed that a single filament winds into a hollow flared base with progressive change to a cylinder. Proteinase K unwound the spinae into proteolysis-resistant filaments. Thermal treatment ripped the spinae into ribbons that were melted with prolonged heating. Circular dichroism spectroscopy revealed a dominant beta-structure of the spinae. Differential scanning calorimetry analyses showed three endothermic transformations at 50–85°C, 98°C and 123°C, respectively. The heating almost completely disintegrated the spinae, abolished the 98°C transition and destroyed the beta-structure. Infrared spectroscopy identified the amide I spectrum maximum at a position similar to that of amyloid fibrils. Therefore, the spinae distinguish from other bacterial appendages, e.g. flagella and stalks, in both the structure and mechanism of assembly. Nature Publishing Group 2012-12-10 /pmc/articles/PMC3517982/ /pubmed/23230515 http://dx.doi.org/10.1038/srep00950 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Bernadac, A. Wu, L.-F. Santini, C.-L. Vidaud, C. Sturgis, J. N. Menguy, N. Bergam, P. Nicoletti, C. Xiao, T. Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp. strain YSCB |
title | Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp. strain YSCB |
title_full | Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp. strain YSCB |
title_fullStr | Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp. strain YSCB |
title_full_unstemmed | Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp. strain YSCB |
title_short | Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp. strain YSCB |
title_sort | structural properties of the tubular appendage spinae from marine bacterium roseobacter sp. strain yscb |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517982/ https://www.ncbi.nlm.nih.gov/pubmed/23230515 http://dx.doi.org/10.1038/srep00950 |
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