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A First Analysis of Metallome Biosignatures of Hyperthermophilic Archaea

To date, no experimental data has been reported for the metallome of hyperthermophilic microorganisms although their metal requirements for growth are known to be unique. Here, experiments were conducted to determine (i) cellular trace metal concentrations of the hyperthermophilic Archaea Methanococ...

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Autores principales: Cameron, Vyllinniskii, House, Christopher H., Brantley, Susan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518089/
https://www.ncbi.nlm.nih.gov/pubmed/23243390
http://dx.doi.org/10.1155/2012/789278
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author Cameron, Vyllinniskii
House, Christopher H.
Brantley, Susan L.
author_facet Cameron, Vyllinniskii
House, Christopher H.
Brantley, Susan L.
author_sort Cameron, Vyllinniskii
collection PubMed
description To date, no experimental data has been reported for the metallome of hyperthermophilic microorganisms although their metal requirements for growth are known to be unique. Here, experiments were conducted to determine (i) cellular trace metal concentrations of the hyperthermophilic Archaea Methanococcus jannaschii and Pyrococcus furiosus, and (ii) a first estimate of the metallome for these hyperthermophilic species via ICP-MS. The metal contents of these cells were compared to parallel experiments using the mesophilic bacterium Escherichia coli grown under aerobic and anaerobic conditions. Fe and Zn were typically the most abundant metals in cells. Metal concentrations for E. coli grown aerobically decreased in the order Fe > Zn > Cu > Mo > Ni > W > Co. In contrast, M. jannaschii and P. furiosus show almost the reverse pattern with elevated Ni, Co, and W concentrations. Of the three organisms, a biosignature is potentially demonstrated for the methanogen M. jannaschii that may, in part, be related to the metallome requirements of methanogenesis. The bioavailability of trace metals more than likely has varied through time. If hyperthermophiles are very ancient, then the trace metal patterns observed here may begin to provide some insights regarding Earth's earliest cells and in turn, early Earth chemistry.
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spelling pubmed-35180892012-12-14 A First Analysis of Metallome Biosignatures of Hyperthermophilic Archaea Cameron, Vyllinniskii House, Christopher H. Brantley, Susan L. Archaea Research Article To date, no experimental data has been reported for the metallome of hyperthermophilic microorganisms although their metal requirements for growth are known to be unique. Here, experiments were conducted to determine (i) cellular trace metal concentrations of the hyperthermophilic Archaea Methanococcus jannaschii and Pyrococcus furiosus, and (ii) a first estimate of the metallome for these hyperthermophilic species via ICP-MS. The metal contents of these cells were compared to parallel experiments using the mesophilic bacterium Escherichia coli grown under aerobic and anaerobic conditions. Fe and Zn were typically the most abundant metals in cells. Metal concentrations for E. coli grown aerobically decreased in the order Fe > Zn > Cu > Mo > Ni > W > Co. In contrast, M. jannaschii and P. furiosus show almost the reverse pattern with elevated Ni, Co, and W concentrations. Of the three organisms, a biosignature is potentially demonstrated for the methanogen M. jannaschii that may, in part, be related to the metallome requirements of methanogenesis. The bioavailability of trace metals more than likely has varied through time. If hyperthermophiles are very ancient, then the trace metal patterns observed here may begin to provide some insights regarding Earth's earliest cells and in turn, early Earth chemistry. Hindawi Publishing Corporation 2012-12-03 /pmc/articles/PMC3518089/ /pubmed/23243390 http://dx.doi.org/10.1155/2012/789278 Text en Copyright © 2012 Vyllinniskii Cameron et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cameron, Vyllinniskii
House, Christopher H.
Brantley, Susan L.
A First Analysis of Metallome Biosignatures of Hyperthermophilic Archaea
title A First Analysis of Metallome Biosignatures of Hyperthermophilic Archaea
title_full A First Analysis of Metallome Biosignatures of Hyperthermophilic Archaea
title_fullStr A First Analysis of Metallome Biosignatures of Hyperthermophilic Archaea
title_full_unstemmed A First Analysis of Metallome Biosignatures of Hyperthermophilic Archaea
title_short A First Analysis of Metallome Biosignatures of Hyperthermophilic Archaea
title_sort first analysis of metallome biosignatures of hyperthermophilic archaea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518089/
https://www.ncbi.nlm.nih.gov/pubmed/23243390
http://dx.doi.org/10.1155/2012/789278
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