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Collagen type I and decorin expression in tenocytes depend on the cell isolation method

BACKROUND: The treatment of rotator cuff tears is still challenging. Tendon tissue engineering (TTE) might be an alternative in future. Tenocytes seem to be the most suitable cell type as they are easy to obtain and no differentiation in vitro is necessary. The aim of this study was to examine, if t...

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Autores principales: Wagenhäuser, Markus U, Pietschmann, Matthias F, Sievers, Birte, Docheva, Denitsa, Schieker, Matthias, Jansson, Volkmar, Müller, Peter E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518183/
https://www.ncbi.nlm.nih.gov/pubmed/22871215
http://dx.doi.org/10.1186/1471-2474-13-140
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author Wagenhäuser, Markus U
Pietschmann, Matthias F
Sievers, Birte
Docheva, Denitsa
Schieker, Matthias
Jansson, Volkmar
Müller, Peter E
author_facet Wagenhäuser, Markus U
Pietschmann, Matthias F
Sievers, Birte
Docheva, Denitsa
Schieker, Matthias
Jansson, Volkmar
Müller, Peter E
author_sort Wagenhäuser, Markus U
collection PubMed
description BACKROUND: The treatment of rotator cuff tears is still challenging. Tendon tissue engineering (TTE) might be an alternative in future. Tenocytes seem to be the most suitable cell type as they are easy to obtain and no differentiation in vitro is necessary. The aim of this study was to examine, if the long head of the biceps tendon (LHB) can deliver viable tenocytes for TTE. In this context, different isolation methods, such as enzymatic digestion (ED) and cell migration (CM), are investigated on differences in gene expression and cell morphology. METHODS: Samples of the LHB were obtained from patients, who underwent surgery for primary shoulder arthroplasty. Using ED as isolation method, 0.2% collagenase I solution was used. Using CM as isolation method, small pieces of minced tendon were put into petri-dishes. After cell cultivation, RT-PCR was performed for collagen type I, collagen type III, decorin, tenascin-C, fibronectin, Scleraxis, tenomodulin, osteopontin and agreccan. RESULTS: The total number of isolated cells, in relation to 1 g of native tissue, was 14 times higher using ED. The time interval for cell isolation was about 17 hours using ED and approximately 50 days using CM. Cell morphology in vitro was similar for both isolation techniques. Higher expression of collagen type I could be observed in tenocyte-like cell cultures (TLCC) using ED as isolation method (p < 0.05), however decorin expression was higher in TLCC using CM as isolation method (p < 0.05). Dedifferentiation potential seemed to be similar for both isolation techniques. CONCLUSION: In summary tenocyte-like cells can be obtained with both isolation methods (ED and CM) from the LHB. As no obvious disadvantage could be seen using ED, this method is more suitable for clinical use, as time for cell isolation is shorter and a remarkably higher number of cells can be obtained. However, both isolation methods can further be improved.
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spelling pubmed-35181832012-12-11 Collagen type I and decorin expression in tenocytes depend on the cell isolation method Wagenhäuser, Markus U Pietschmann, Matthias F Sievers, Birte Docheva, Denitsa Schieker, Matthias Jansson, Volkmar Müller, Peter E BMC Musculoskelet Disord Research Article BACKROUND: The treatment of rotator cuff tears is still challenging. Tendon tissue engineering (TTE) might be an alternative in future. Tenocytes seem to be the most suitable cell type as they are easy to obtain and no differentiation in vitro is necessary. The aim of this study was to examine, if the long head of the biceps tendon (LHB) can deliver viable tenocytes for TTE. In this context, different isolation methods, such as enzymatic digestion (ED) and cell migration (CM), are investigated on differences in gene expression and cell morphology. METHODS: Samples of the LHB were obtained from patients, who underwent surgery for primary shoulder arthroplasty. Using ED as isolation method, 0.2% collagenase I solution was used. Using CM as isolation method, small pieces of minced tendon were put into petri-dishes. After cell cultivation, RT-PCR was performed for collagen type I, collagen type III, decorin, tenascin-C, fibronectin, Scleraxis, tenomodulin, osteopontin and agreccan. RESULTS: The total number of isolated cells, in relation to 1 g of native tissue, was 14 times higher using ED. The time interval for cell isolation was about 17 hours using ED and approximately 50 days using CM. Cell morphology in vitro was similar for both isolation techniques. Higher expression of collagen type I could be observed in tenocyte-like cell cultures (TLCC) using ED as isolation method (p < 0.05), however decorin expression was higher in TLCC using CM as isolation method (p < 0.05). Dedifferentiation potential seemed to be similar for both isolation techniques. CONCLUSION: In summary tenocyte-like cells can be obtained with both isolation methods (ED and CM) from the LHB. As no obvious disadvantage could be seen using ED, this method is more suitable for clinical use, as time for cell isolation is shorter and a remarkably higher number of cells can be obtained. However, both isolation methods can further be improved. BioMed Central 2012-08-08 /pmc/articles/PMC3518183/ /pubmed/22871215 http://dx.doi.org/10.1186/1471-2474-13-140 Text en Copyright ©2012 Wagenhäuser et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wagenhäuser, Markus U
Pietschmann, Matthias F
Sievers, Birte
Docheva, Denitsa
Schieker, Matthias
Jansson, Volkmar
Müller, Peter E
Collagen type I and decorin expression in tenocytes depend on the cell isolation method
title Collagen type I and decorin expression in tenocytes depend on the cell isolation method
title_full Collagen type I and decorin expression in tenocytes depend on the cell isolation method
title_fullStr Collagen type I and decorin expression in tenocytes depend on the cell isolation method
title_full_unstemmed Collagen type I and decorin expression in tenocytes depend on the cell isolation method
title_short Collagen type I and decorin expression in tenocytes depend on the cell isolation method
title_sort collagen type i and decorin expression in tenocytes depend on the cell isolation method
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518183/
https://www.ncbi.nlm.nih.gov/pubmed/22871215
http://dx.doi.org/10.1186/1471-2474-13-140
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