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Mass landscapes of seven scorpion species: The first analyses of Australian species with 1,5-DAN matrix
Scorpion venoms have been studied for over fifty years; however, the majority of research has focussed primarily on medically important Buthidae species. Additionally, venoms of the estimated 200 species of scorpion native to Australia have received very little attention. The first venom mass profil...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Library Publishing Media
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518322/ https://www.ncbi.nlm.nih.gov/pubmed/23236582 |
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author | Smith, Jennifer J Jones, Alun Alewood, Paul F |
author_facet | Smith, Jennifer J Jones, Alun Alewood, Paul F |
author_sort | Smith, Jennifer J |
collection | PubMed |
description | Scorpion venoms have been studied for over fifty years; however, the majority of research has focussed primarily on medically important Buthidae species. Additionally, venoms of the estimated 200 species of scorpion native to Australia have received very little attention. The first venom mass profiles of six non-buthid and one buthid scorpion species are presented herein, four of which are endemic to Australia. While masses under 5 kDa dominated the venoms of all species, the buthid venom contained considerably more masses between 7 and 8 kDa than those of the non-buthids, corroborating the emergent trend that buthids are richer in long-chain neurotoxins than non-buthids. The Australian scorpion venom fractions were also analysed with the relatively new MALDI-ToF matrix 1,5-DAN. Over forty partial sequences were obtained, the majority of which are homologous to scorpion antimicrobials such as opistoporin and IsCT2. Overall, this study is the single most comprehensive mass spectrometric analysis of scorpion venom landscapes to date and provides an insight into untapped Australian species. |
format | Online Article Text |
id | pubmed-3518322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Library Publishing Media |
record_format | MEDLINE/PubMed |
spelling | pubmed-35183222012-12-12 Mass landscapes of seven scorpion species: The first analyses of Australian species with 1,5-DAN matrix Smith, Jennifer J Jones, Alun Alewood, Paul F J Venom Res Research Report Scorpion venoms have been studied for over fifty years; however, the majority of research has focussed primarily on medically important Buthidae species. Additionally, venoms of the estimated 200 species of scorpion native to Australia have received very little attention. The first venom mass profiles of six non-buthid and one buthid scorpion species are presented herein, four of which are endemic to Australia. While masses under 5 kDa dominated the venoms of all species, the buthid venom contained considerably more masses between 7 and 8 kDa than those of the non-buthids, corroborating the emergent trend that buthids are richer in long-chain neurotoxins than non-buthids. The Australian scorpion venom fractions were also analysed with the relatively new MALDI-ToF matrix 1,5-DAN. Over forty partial sequences were obtained, the majority of which are homologous to scorpion antimicrobials such as opistoporin and IsCT2. Overall, this study is the single most comprehensive mass spectrometric analysis of scorpion venom landscapes to date and provides an insight into untapped Australian species. Library Publishing Media 2012-10-23 /pmc/articles/PMC3518322/ /pubmed/23236582 Text en © Copyright The Author(s) http://creativecommons.org/licenses/by-nc/2.5 Published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details. |
spellingShingle | Research Report Smith, Jennifer J Jones, Alun Alewood, Paul F Mass landscapes of seven scorpion species: The first analyses of Australian species with 1,5-DAN matrix |
title | Mass landscapes of seven scorpion species: The first analyses of Australian species with 1,5-DAN matrix |
title_full | Mass landscapes of seven scorpion species: The first analyses of Australian species with 1,5-DAN matrix |
title_fullStr | Mass landscapes of seven scorpion species: The first analyses of Australian species with 1,5-DAN matrix |
title_full_unstemmed | Mass landscapes of seven scorpion species: The first analyses of Australian species with 1,5-DAN matrix |
title_short | Mass landscapes of seven scorpion species: The first analyses of Australian species with 1,5-DAN matrix |
title_sort | mass landscapes of seven scorpion species: the first analyses of australian species with 1,5-dan matrix |
topic | Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518322/ https://www.ncbi.nlm.nih.gov/pubmed/23236582 |
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