Cargando…

Integrase-Mediated Recombination of the veb1 Gene Cassette Encoding an Extended-Spectrum β-Lactamase

The veb1 gene cassette encodes the extended spectrum β-lactamase, VEB-1 that is increasingly isolated from worldwide Gram-negative rods. Veb1 is commonly inserted into the variable region of different class 1 integrons in which it is always associated with a downstream-located aadB gene cassette enc...

Descripción completa

Detalles Bibliográficos
Autores principales: Aubert, Daniel, Naas, Thierry, Nordmann, Patrice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518468/
https://www.ncbi.nlm.nih.gov/pubmed/23251590
http://dx.doi.org/10.1371/journal.pone.0051602
_version_ 1782252559916859392
author Aubert, Daniel
Naas, Thierry
Nordmann, Patrice
author_facet Aubert, Daniel
Naas, Thierry
Nordmann, Patrice
author_sort Aubert, Daniel
collection PubMed
description The veb1 gene cassette encodes the extended spectrum β-lactamase, VEB-1 that is increasingly isolated from worldwide Gram-negative rods. Veb1 is commonly inserted into the variable region of different class 1 integrons in which it is always associated with a downstream-located aadB gene cassette encoding an aminoglycoside adenylyltransferase. In Pseudomonas aeruginosa, the majority of veb1-containing integrons also carry an insertion sequence, IS1999 that is inserted upstream of the veb1 gene cassette and disrupts the integron specific recombination site, attI1. Investigation of the recombination properties of the sites surrounding veb1 revealed that insertion of IS1999 reduces significantly the recombination frequency of attI1 and that veb1 attC is not efficient for recombination in contrast to aadB attC. Subsequent sequence optimisation of veb1 attC by mutagenesis, into a more consensual attC site resembling aadB attC, successfully improved recombination efficiency. Overall, this work gives some insights into the organisation of veb1-containing integrons. We propose that IS1999 and the nature of veb1 attC stabilize the veb1 gene cassette environment likely by impairing recombination events upstream or downstream of veb1, respectively.
format Online
Article
Text
id pubmed-3518468
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-35184682012-12-18 Integrase-Mediated Recombination of the veb1 Gene Cassette Encoding an Extended-Spectrum β-Lactamase Aubert, Daniel Naas, Thierry Nordmann, Patrice PLoS One Research Article The veb1 gene cassette encodes the extended spectrum β-lactamase, VEB-1 that is increasingly isolated from worldwide Gram-negative rods. Veb1 is commonly inserted into the variable region of different class 1 integrons in which it is always associated with a downstream-located aadB gene cassette encoding an aminoglycoside adenylyltransferase. In Pseudomonas aeruginosa, the majority of veb1-containing integrons also carry an insertion sequence, IS1999 that is inserted upstream of the veb1 gene cassette and disrupts the integron specific recombination site, attI1. Investigation of the recombination properties of the sites surrounding veb1 revealed that insertion of IS1999 reduces significantly the recombination frequency of attI1 and that veb1 attC is not efficient for recombination in contrast to aadB attC. Subsequent sequence optimisation of veb1 attC by mutagenesis, into a more consensual attC site resembling aadB attC, successfully improved recombination efficiency. Overall, this work gives some insights into the organisation of veb1-containing integrons. We propose that IS1999 and the nature of veb1 attC stabilize the veb1 gene cassette environment likely by impairing recombination events upstream or downstream of veb1, respectively. Public Library of Science 2012-12-10 /pmc/articles/PMC3518468/ /pubmed/23251590 http://dx.doi.org/10.1371/journal.pone.0051602 Text en © 2012 Aubert et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Aubert, Daniel
Naas, Thierry
Nordmann, Patrice
Integrase-Mediated Recombination of the veb1 Gene Cassette Encoding an Extended-Spectrum β-Lactamase
title Integrase-Mediated Recombination of the veb1 Gene Cassette Encoding an Extended-Spectrum β-Lactamase
title_full Integrase-Mediated Recombination of the veb1 Gene Cassette Encoding an Extended-Spectrum β-Lactamase
title_fullStr Integrase-Mediated Recombination of the veb1 Gene Cassette Encoding an Extended-Spectrum β-Lactamase
title_full_unstemmed Integrase-Mediated Recombination of the veb1 Gene Cassette Encoding an Extended-Spectrum β-Lactamase
title_short Integrase-Mediated Recombination of the veb1 Gene Cassette Encoding an Extended-Spectrum β-Lactamase
title_sort integrase-mediated recombination of the veb1 gene cassette encoding an extended-spectrum β-lactamase
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518468/
https://www.ncbi.nlm.nih.gov/pubmed/23251590
http://dx.doi.org/10.1371/journal.pone.0051602
work_keys_str_mv AT aubertdaniel integrasemediatedrecombinationoftheveb1genecassetteencodinganextendedspectrumblactamase
AT naasthierry integrasemediatedrecombinationoftheveb1genecassetteencodinganextendedspectrumblactamase
AT nordmannpatrice integrasemediatedrecombinationoftheveb1genecassetteencodinganextendedspectrumblactamase