Two-photon optogenetics of dendritic spines and neural circuits in 3D

We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We apply the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual...

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Detalles Bibliográficos
Autores principales: Packer, Adam M., Peterka, Darcy S., Hirtz, Jan J., Prakash, Rohit, Deisseroth, Karl, Yuste, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518588/
https://www.ncbi.nlm.nih.gov/pubmed/23142873
http://dx.doi.org/10.1038/nmeth.2249
Descripción
Sumario:We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We apply the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator we split the laser beam onto several neurons and perform simultaneous optogenetic activation of selected neurons in three dimensions.

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