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Peroxisome Proliferator-Activated Receptor Gamma Exacerbates Concanavalin A-Induced Liver Injury via Suppressing the Translocation of NF-κB into the Nucleus
Peroxisome proliferator-activated receptor-γ (PPARγ) has been reported to reduce inflammation and attenuate fibrosis in the liver. In this study, we investigated the effects of PPARγ on the liver injury induced by 20 mg/kg Concanavalin A (Con A). The mice were administered one of the three types of...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518969/ https://www.ncbi.nlm.nih.gov/pubmed/23251143 http://dx.doi.org/10.1155/2012/940384 |
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author | Ogawa, Yuji Yoneda, Masato Tomeno, Wataru Imajo, Kento Shinohara, Yoshiyasu Fujita, Koji Shibata, Wataru Kirikoshi, Hiroyuki Saito, Satoru Wada, Koichiro Maeda, Shin Nakajima, Atsushi |
author_facet | Ogawa, Yuji Yoneda, Masato Tomeno, Wataru Imajo, Kento Shinohara, Yoshiyasu Fujita, Koji Shibata, Wataru Kirikoshi, Hiroyuki Saito, Satoru Wada, Koichiro Maeda, Shin Nakajima, Atsushi |
author_sort | Ogawa, Yuji |
collection | PubMed |
description | Peroxisome proliferator-activated receptor-γ (PPARγ) has been reported to reduce inflammation and attenuate fibrosis in the liver. In this study, we investigated the effects of PPARγ on the liver injury induced by 20 mg/kg Concanavalin A (Con A). The mice were administered one of the three types of PPARγ ligands (pioglitazone, ciglitazone, and troglitazone) for 1 week, and the serum alanine aminotransferase (ALT) levels at 20 h after Con A injection were significantly elevated in the PPARγ ligand-treated mice. Furthermore, the serum ALT levels after Con A injection in the PPARγ hetero-knock-out mice (PPARγ (+/−) mice) were lower than those in the wild-type mice (WT mice). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) revealed extensive liver damage induced by Con A in the pioglitazone-treated mice. Electrophoresis mobility shift assay (EMSA) revealed that activation of translocation of nuclear factor- (NF-) κB, which is a suppressor of apoptosis, in the nucleus of the hepatocytes was suppressed in the pioglitazone-treated mice after Con A injection. In this study, we showed that PPARγ exacerbated Con A-induced liver injury via suppressing the translocation of NF-κB into the nucleus, thereby inhibiting the suppression of liver cell apoptosis. |
format | Online Article Text |
id | pubmed-3518969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-35189692012-12-18 Peroxisome Proliferator-Activated Receptor Gamma Exacerbates Concanavalin A-Induced Liver Injury via Suppressing the Translocation of NF-κB into the Nucleus Ogawa, Yuji Yoneda, Masato Tomeno, Wataru Imajo, Kento Shinohara, Yoshiyasu Fujita, Koji Shibata, Wataru Kirikoshi, Hiroyuki Saito, Satoru Wada, Koichiro Maeda, Shin Nakajima, Atsushi PPAR Res Research Article Peroxisome proliferator-activated receptor-γ (PPARγ) has been reported to reduce inflammation and attenuate fibrosis in the liver. In this study, we investigated the effects of PPARγ on the liver injury induced by 20 mg/kg Concanavalin A (Con A). The mice were administered one of the three types of PPARγ ligands (pioglitazone, ciglitazone, and troglitazone) for 1 week, and the serum alanine aminotransferase (ALT) levels at 20 h after Con A injection were significantly elevated in the PPARγ ligand-treated mice. Furthermore, the serum ALT levels after Con A injection in the PPARγ hetero-knock-out mice (PPARγ (+/−) mice) were lower than those in the wild-type mice (WT mice). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) revealed extensive liver damage induced by Con A in the pioglitazone-treated mice. Electrophoresis mobility shift assay (EMSA) revealed that activation of translocation of nuclear factor- (NF-) κB, which is a suppressor of apoptosis, in the nucleus of the hepatocytes was suppressed in the pioglitazone-treated mice after Con A injection. In this study, we showed that PPARγ exacerbated Con A-induced liver injury via suppressing the translocation of NF-κB into the nucleus, thereby inhibiting the suppression of liver cell apoptosis. Hindawi Publishing Corporation 2012 2012-12-03 /pmc/articles/PMC3518969/ /pubmed/23251143 http://dx.doi.org/10.1155/2012/940384 Text en Copyright © 2012 Yuji Ogawa et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ogawa, Yuji Yoneda, Masato Tomeno, Wataru Imajo, Kento Shinohara, Yoshiyasu Fujita, Koji Shibata, Wataru Kirikoshi, Hiroyuki Saito, Satoru Wada, Koichiro Maeda, Shin Nakajima, Atsushi Peroxisome Proliferator-Activated Receptor Gamma Exacerbates Concanavalin A-Induced Liver Injury via Suppressing the Translocation of NF-κB into the Nucleus |
title | Peroxisome Proliferator-Activated Receptor Gamma Exacerbates Concanavalin A-Induced Liver Injury via Suppressing the Translocation of NF-κB into the Nucleus |
title_full | Peroxisome Proliferator-Activated Receptor Gamma Exacerbates Concanavalin A-Induced Liver Injury via Suppressing the Translocation of NF-κB into the Nucleus |
title_fullStr | Peroxisome Proliferator-Activated Receptor Gamma Exacerbates Concanavalin A-Induced Liver Injury via Suppressing the Translocation of NF-κB into the Nucleus |
title_full_unstemmed | Peroxisome Proliferator-Activated Receptor Gamma Exacerbates Concanavalin A-Induced Liver Injury via Suppressing the Translocation of NF-κB into the Nucleus |
title_short | Peroxisome Proliferator-Activated Receptor Gamma Exacerbates Concanavalin A-Induced Liver Injury via Suppressing the Translocation of NF-κB into the Nucleus |
title_sort | peroxisome proliferator-activated receptor gamma exacerbates concanavalin a-induced liver injury via suppressing the translocation of nf-κb into the nucleus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518969/ https://www.ncbi.nlm.nih.gov/pubmed/23251143 http://dx.doi.org/10.1155/2012/940384 |
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