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Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms
Specific neurochemicals measured with proton magnetic resonance spectroscopy ((1)H-MRS) may serve as biomarkers of pathological mechanism in the brain. We used high field in vivo (1)H-MRS to measure a detailed neurochemical profile after experimental traumatic brain injury (TBI) in rats. We characte...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519407/ https://www.ncbi.nlm.nih.gov/pubmed/22892723 http://dx.doi.org/10.1038/jcbfm.2012.114 |
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author | Harris, Janna L Yeh, Hung-Wen Choi, In-Young Lee, Phil Berman, Nancy E Swerdlow, Russell H Craciunas, Sorin C Brooks, William M |
author_facet | Harris, Janna L Yeh, Hung-Wen Choi, In-Young Lee, Phil Berman, Nancy E Swerdlow, Russell H Craciunas, Sorin C Brooks, William M |
author_sort | Harris, Janna L |
collection | PubMed |
description | Specific neurochemicals measured with proton magnetic resonance spectroscopy ((1)H-MRS) may serve as biomarkers of pathological mechanism in the brain. We used high field in vivo (1)H-MRS to measure a detailed neurochemical profile after experimental traumatic brain injury (TBI) in rats. We characterized neurochemical changes in the contused cortex and the normal-appearing perilesional hippocampus over a time course from 1 hour to 2 weeks after injury. We found significant changes in 19 out of 20 neurochemicals in the cortex, and 9 out of 20 neurochemicals in the hippocampus. These changes provide evidence of altered cellular metabolic status after TBI, with specific compounds proposed to reflect edema, excitotoxicity, neuronal and glial integrity, mitochondrial status and bioenergetics, oxidative stress, inflammation, and cell membrane disruption. Our results support the utility of (1)H-MRS for monitoring cellular mechanisms of TBI pathology in animal models, and the potential of this approach for preclinical evaluation of novel therapies. |
format | Online Article Text |
id | pubmed-3519407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-35194072012-12-11 Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms Harris, Janna L Yeh, Hung-Wen Choi, In-Young Lee, Phil Berman, Nancy E Swerdlow, Russell H Craciunas, Sorin C Brooks, William M J Cereb Blood Flow Metab Original Article Specific neurochemicals measured with proton magnetic resonance spectroscopy ((1)H-MRS) may serve as biomarkers of pathological mechanism in the brain. We used high field in vivo (1)H-MRS to measure a detailed neurochemical profile after experimental traumatic brain injury (TBI) in rats. We characterized neurochemical changes in the contused cortex and the normal-appearing perilesional hippocampus over a time course from 1 hour to 2 weeks after injury. We found significant changes in 19 out of 20 neurochemicals in the cortex, and 9 out of 20 neurochemicals in the hippocampus. These changes provide evidence of altered cellular metabolic status after TBI, with specific compounds proposed to reflect edema, excitotoxicity, neuronal and glial integrity, mitochondrial status and bioenergetics, oxidative stress, inflammation, and cell membrane disruption. Our results support the utility of (1)H-MRS for monitoring cellular mechanisms of TBI pathology in animal models, and the potential of this approach for preclinical evaluation of novel therapies. Nature Publishing Group 2012-12 2012-08-15 /pmc/articles/PMC3519407/ /pubmed/22892723 http://dx.doi.org/10.1038/jcbfm.2012.114 Text en Copyright © 2012 International Society for Cerebral Blood Flow & Metabolism, Inc. http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Original Article Harris, Janna L Yeh, Hung-Wen Choi, In-Young Lee, Phil Berman, Nancy E Swerdlow, Russell H Craciunas, Sorin C Brooks, William M Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms |
title | Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms |
title_full | Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms |
title_fullStr | Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms |
title_full_unstemmed | Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms |
title_short | Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms |
title_sort | altered neurochemical profile after traumatic brain injury: (1)h-mrs biomarkers of pathological mechanisms |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519407/ https://www.ncbi.nlm.nih.gov/pubmed/22892723 http://dx.doi.org/10.1038/jcbfm.2012.114 |
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