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Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms

Specific neurochemicals measured with proton magnetic resonance spectroscopy ((1)H-MRS) may serve as biomarkers of pathological mechanism in the brain. We used high field in vivo (1)H-MRS to measure a detailed neurochemical profile after experimental traumatic brain injury (TBI) in rats. We characte...

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Autores principales: Harris, Janna L, Yeh, Hung-Wen, Choi, In-Young, Lee, Phil, Berman, Nancy E, Swerdlow, Russell H, Craciunas, Sorin C, Brooks, William M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519407/
https://www.ncbi.nlm.nih.gov/pubmed/22892723
http://dx.doi.org/10.1038/jcbfm.2012.114
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author Harris, Janna L
Yeh, Hung-Wen
Choi, In-Young
Lee, Phil
Berman, Nancy E
Swerdlow, Russell H
Craciunas, Sorin C
Brooks, William M
author_facet Harris, Janna L
Yeh, Hung-Wen
Choi, In-Young
Lee, Phil
Berman, Nancy E
Swerdlow, Russell H
Craciunas, Sorin C
Brooks, William M
author_sort Harris, Janna L
collection PubMed
description Specific neurochemicals measured with proton magnetic resonance spectroscopy ((1)H-MRS) may serve as biomarkers of pathological mechanism in the brain. We used high field in vivo (1)H-MRS to measure a detailed neurochemical profile after experimental traumatic brain injury (TBI) in rats. We characterized neurochemical changes in the contused cortex and the normal-appearing perilesional hippocampus over a time course from 1 hour to 2 weeks after injury. We found significant changes in 19 out of 20 neurochemicals in the cortex, and 9 out of 20 neurochemicals in the hippocampus. These changes provide evidence of altered cellular metabolic status after TBI, with specific compounds proposed to reflect edema, excitotoxicity, neuronal and glial integrity, mitochondrial status and bioenergetics, oxidative stress, inflammation, and cell membrane disruption. Our results support the utility of (1)H-MRS for monitoring cellular mechanisms of TBI pathology in animal models, and the potential of this approach for preclinical evaluation of novel therapies.
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spelling pubmed-35194072012-12-11 Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms Harris, Janna L Yeh, Hung-Wen Choi, In-Young Lee, Phil Berman, Nancy E Swerdlow, Russell H Craciunas, Sorin C Brooks, William M J Cereb Blood Flow Metab Original Article Specific neurochemicals measured with proton magnetic resonance spectroscopy ((1)H-MRS) may serve as biomarkers of pathological mechanism in the brain. We used high field in vivo (1)H-MRS to measure a detailed neurochemical profile after experimental traumatic brain injury (TBI) in rats. We characterized neurochemical changes in the contused cortex and the normal-appearing perilesional hippocampus over a time course from 1 hour to 2 weeks after injury. We found significant changes in 19 out of 20 neurochemicals in the cortex, and 9 out of 20 neurochemicals in the hippocampus. These changes provide evidence of altered cellular metabolic status after TBI, with specific compounds proposed to reflect edema, excitotoxicity, neuronal and glial integrity, mitochondrial status and bioenergetics, oxidative stress, inflammation, and cell membrane disruption. Our results support the utility of (1)H-MRS for monitoring cellular mechanisms of TBI pathology in animal models, and the potential of this approach for preclinical evaluation of novel therapies. Nature Publishing Group 2012-12 2012-08-15 /pmc/articles/PMC3519407/ /pubmed/22892723 http://dx.doi.org/10.1038/jcbfm.2012.114 Text en Copyright © 2012 International Society for Cerebral Blood Flow & Metabolism, Inc. http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Original Article
Harris, Janna L
Yeh, Hung-Wen
Choi, In-Young
Lee, Phil
Berman, Nancy E
Swerdlow, Russell H
Craciunas, Sorin C
Brooks, William M
Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms
title Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms
title_full Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms
title_fullStr Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms
title_full_unstemmed Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms
title_short Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms
title_sort altered neurochemical profile after traumatic brain injury: (1)h-mrs biomarkers of pathological mechanisms
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519407/
https://www.ncbi.nlm.nih.gov/pubmed/22892723
http://dx.doi.org/10.1038/jcbfm.2012.114
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