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Timosaponin-BII inhibits the up-regulation of BACE1 induced by Ferric Chloride in rat retina

BACKGROUND: Our previous studies indicated that oxidative stress up-regulated the expression of β-amyloid precursor protein cleavage enzyme-1 (BACE1) in rat retina. Pharmacological reports have shown Timosaponin-BII, a purified extract originating from Chinese medical herb Rhizoma Anemarrhenae, is c...

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Autores principales: Huang, Ju-Fang, Shang, Lei, Liu, Pei, Zhang, Meng-Qi, Chen, Shuang, Chen, Dan, Fan, Chun-Ling, Wang, Hui, Xiong, Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519506/
https://www.ncbi.nlm.nih.gov/pubmed/23082924
http://dx.doi.org/10.1186/1472-6882-12-189
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author Huang, Ju-Fang
Shang, Lei
Liu, Pei
Zhang, Meng-Qi
Chen, Shuang
Chen, Dan
Fan, Chun-Ling
Wang, Hui
Xiong, Kun
author_facet Huang, Ju-Fang
Shang, Lei
Liu, Pei
Zhang, Meng-Qi
Chen, Shuang
Chen, Dan
Fan, Chun-Ling
Wang, Hui
Xiong, Kun
author_sort Huang, Ju-Fang
collection PubMed
description BACKGROUND: Our previous studies indicated that oxidative stress up-regulated the expression of β-amyloid precursor protein cleavage enzyme-1 (BACE1) in rat retina. Pharmacological reports have shown Timosaponin-BII, a purified extract originating from Chinese medical herb Rhizoma Anemarrhenae, is characterized as an antioxidant. Our present study aimed to determine whether Timosaponin-BII affected the expression of BACE1, β-amyloid precursor protein cleavage production of Aβ1-40 and β-C-terminal fragment (β-CTF) in rat retina, which were pre-treated with the oxidizing agent (solution of FeCl(3)). RESULTS: Few distinctions of BACE1 distribution were observed among all groups (normal control group, model group, Timosaponin-BII treated and vehicle control groups). Rat retinas in model group and vehicle control group manifested an apparent up-regulation of BACE1 expression. Meanwhile, the level of malonaldehyde (MDA), Aβ1-40 and β-CTF were increased. However, when comparing with the vehicle control group, the retinas in Timosaponin-BII treated group showed significantly less BACE1 (p<0.05) and accumulated less Aβ1-40 or β-CTF (p<0.05). It also showed significantly decreased level of MDA (p<0.05) and prolonged partial thromboplastin time (p<0.05). CONCLUSION: Our data suggested that Timosaponin-BII remarkably inhibited the up-regulation of BACE1 and reduced the over-production of β-CTF and Aβ in rat retina, which was induced by FeCl(3). The mechanism of Timosaponin-BII on BACE1 expression may be related to its antioxidant property.
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spelling pubmed-35195062012-12-12 Timosaponin-BII inhibits the up-regulation of BACE1 induced by Ferric Chloride in rat retina Huang, Ju-Fang Shang, Lei Liu, Pei Zhang, Meng-Qi Chen, Shuang Chen, Dan Fan, Chun-Ling Wang, Hui Xiong, Kun BMC Complement Altern Med Research Article BACKGROUND: Our previous studies indicated that oxidative stress up-regulated the expression of β-amyloid precursor protein cleavage enzyme-1 (BACE1) in rat retina. Pharmacological reports have shown Timosaponin-BII, a purified extract originating from Chinese medical herb Rhizoma Anemarrhenae, is characterized as an antioxidant. Our present study aimed to determine whether Timosaponin-BII affected the expression of BACE1, β-amyloid precursor protein cleavage production of Aβ1-40 and β-C-terminal fragment (β-CTF) in rat retina, which were pre-treated with the oxidizing agent (solution of FeCl(3)). RESULTS: Few distinctions of BACE1 distribution were observed among all groups (normal control group, model group, Timosaponin-BII treated and vehicle control groups). Rat retinas in model group and vehicle control group manifested an apparent up-regulation of BACE1 expression. Meanwhile, the level of malonaldehyde (MDA), Aβ1-40 and β-CTF were increased. However, when comparing with the vehicle control group, the retinas in Timosaponin-BII treated group showed significantly less BACE1 (p<0.05) and accumulated less Aβ1-40 or β-CTF (p<0.05). It also showed significantly decreased level of MDA (p<0.05) and prolonged partial thromboplastin time (p<0.05). CONCLUSION: Our data suggested that Timosaponin-BII remarkably inhibited the up-regulation of BACE1 and reduced the over-production of β-CTF and Aβ in rat retina, which was induced by FeCl(3). The mechanism of Timosaponin-BII on BACE1 expression may be related to its antioxidant property. BioMed Central 2012-10-22 /pmc/articles/PMC3519506/ /pubmed/23082924 http://dx.doi.org/10.1186/1472-6882-12-189 Text en Copyright ©2012 Huang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Huang, Ju-Fang
Shang, Lei
Liu, Pei
Zhang, Meng-Qi
Chen, Shuang
Chen, Dan
Fan, Chun-Ling
Wang, Hui
Xiong, Kun
Timosaponin-BII inhibits the up-regulation of BACE1 induced by Ferric Chloride in rat retina
title Timosaponin-BII inhibits the up-regulation of BACE1 induced by Ferric Chloride in rat retina
title_full Timosaponin-BII inhibits the up-regulation of BACE1 induced by Ferric Chloride in rat retina
title_fullStr Timosaponin-BII inhibits the up-regulation of BACE1 induced by Ferric Chloride in rat retina
title_full_unstemmed Timosaponin-BII inhibits the up-regulation of BACE1 induced by Ferric Chloride in rat retina
title_short Timosaponin-BII inhibits the up-regulation of BACE1 induced by Ferric Chloride in rat retina
title_sort timosaponin-bii inhibits the up-regulation of bace1 induced by ferric chloride in rat retina
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519506/
https://www.ncbi.nlm.nih.gov/pubmed/23082924
http://dx.doi.org/10.1186/1472-6882-12-189
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