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The Ileal Lipid Binding Protein Is Required for Efficient Absorption and Transport of Bile Acids in the Distal Portion of the Murine Small Intestine

The ileal lipid binding protein (ilbp) is a cytoplasmic protein that binds bile acids with high affinity. However evidence demonstrating the role of this protein in bile acid transport and homeostasis is missing. We created a mouse strain lacking ilbp (Fabp6(−/−) mice) and assessed the impact of ilb...

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Autores principales: Praslickova, Dana, Torchia, Enrique C., Sugiyama, Michael G., Magrane, Elijah J., Zwicker, Brittnee L., Kolodzieyski, Lev, Agellon, Luis B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519535/
https://www.ncbi.nlm.nih.gov/pubmed/23251388
http://dx.doi.org/10.1371/journal.pone.0050810
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author Praslickova, Dana
Torchia, Enrique C.
Sugiyama, Michael G.
Magrane, Elijah J.
Zwicker, Brittnee L.
Kolodzieyski, Lev
Agellon, Luis B.
author_facet Praslickova, Dana
Torchia, Enrique C.
Sugiyama, Michael G.
Magrane, Elijah J.
Zwicker, Brittnee L.
Kolodzieyski, Lev
Agellon, Luis B.
author_sort Praslickova, Dana
collection PubMed
description The ileal lipid binding protein (ilbp) is a cytoplasmic protein that binds bile acids with high affinity. However evidence demonstrating the role of this protein in bile acid transport and homeostasis is missing. We created a mouse strain lacking ilbp (Fabp6(−/−) mice) and assessed the impact of ilbp deficiency on bile acid homeostasis and transport in vivo. Elimination of ilbp increased fecal bile acid excretion (54.2%, P<0.05) in female but not male Fabp6(−/−) mice. The activity of cholesterol 7α-hydroxylase (cyp7a1), the rate-controlling enzyme of the classical bile acid biosynthetic pathway, was significantly increased in female (63.5%, P<0.05) but not in male Fabp6(−/−) mice. The amount of [(3)H]taurocholic acid (TCA) excreted by 24 h after oral administration was 102% (P<0.025) higher for female Fabp6(−/−) mice whereas it was 57.3% (P<0.01) lower for male Fabp6(−/−) mice, compared to wild-type mice. The retained fraction of the [(3)H]TCA localized in the small and large intestines was increased by 22% (P<0.02) and decreased by 62.7% (P<0.01), respectively, in male Fabp6(−/−) mice relative wild-type mice, whereas no changes were seen in female Fabp6(−/−) mice. Mucosal to serosal bile acid transport using everted distal gut sacs was decreased by 74% (P<0.03) in both sexes of Fabp6(−/−) mice as compared to wild-type mice. The results demonstrate that ilbp is involved in the apical to basolateral transport of bile acids in ileal enterocytes, and is vital for the maintenance of bile acid homeostasis in the enterohepatic circulation (EHC) in mice.
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spelling pubmed-35195352012-12-18 The Ileal Lipid Binding Protein Is Required for Efficient Absorption and Transport of Bile Acids in the Distal Portion of the Murine Small Intestine Praslickova, Dana Torchia, Enrique C. Sugiyama, Michael G. Magrane, Elijah J. Zwicker, Brittnee L. Kolodzieyski, Lev Agellon, Luis B. PLoS One Research Article The ileal lipid binding protein (ilbp) is a cytoplasmic protein that binds bile acids with high affinity. However evidence demonstrating the role of this protein in bile acid transport and homeostasis is missing. We created a mouse strain lacking ilbp (Fabp6(−/−) mice) and assessed the impact of ilbp deficiency on bile acid homeostasis and transport in vivo. Elimination of ilbp increased fecal bile acid excretion (54.2%, P<0.05) in female but not male Fabp6(−/−) mice. The activity of cholesterol 7α-hydroxylase (cyp7a1), the rate-controlling enzyme of the classical bile acid biosynthetic pathway, was significantly increased in female (63.5%, P<0.05) but not in male Fabp6(−/−) mice. The amount of [(3)H]taurocholic acid (TCA) excreted by 24 h after oral administration was 102% (P<0.025) higher for female Fabp6(−/−) mice whereas it was 57.3% (P<0.01) lower for male Fabp6(−/−) mice, compared to wild-type mice. The retained fraction of the [(3)H]TCA localized in the small and large intestines was increased by 22% (P<0.02) and decreased by 62.7% (P<0.01), respectively, in male Fabp6(−/−) mice relative wild-type mice, whereas no changes were seen in female Fabp6(−/−) mice. Mucosal to serosal bile acid transport using everted distal gut sacs was decreased by 74% (P<0.03) in both sexes of Fabp6(−/−) mice as compared to wild-type mice. The results demonstrate that ilbp is involved in the apical to basolateral transport of bile acids in ileal enterocytes, and is vital for the maintenance of bile acid homeostasis in the enterohepatic circulation (EHC) in mice. Public Library of Science 2012-12-10 /pmc/articles/PMC3519535/ /pubmed/23251388 http://dx.doi.org/10.1371/journal.pone.0050810 Text en © 2012 Praslickova et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Praslickova, Dana
Torchia, Enrique C.
Sugiyama, Michael G.
Magrane, Elijah J.
Zwicker, Brittnee L.
Kolodzieyski, Lev
Agellon, Luis B.
The Ileal Lipid Binding Protein Is Required for Efficient Absorption and Transport of Bile Acids in the Distal Portion of the Murine Small Intestine
title The Ileal Lipid Binding Protein Is Required for Efficient Absorption and Transport of Bile Acids in the Distal Portion of the Murine Small Intestine
title_full The Ileal Lipid Binding Protein Is Required for Efficient Absorption and Transport of Bile Acids in the Distal Portion of the Murine Small Intestine
title_fullStr The Ileal Lipid Binding Protein Is Required for Efficient Absorption and Transport of Bile Acids in the Distal Portion of the Murine Small Intestine
title_full_unstemmed The Ileal Lipid Binding Protein Is Required for Efficient Absorption and Transport of Bile Acids in the Distal Portion of the Murine Small Intestine
title_short The Ileal Lipid Binding Protein Is Required for Efficient Absorption and Transport of Bile Acids in the Distal Portion of the Murine Small Intestine
title_sort ileal lipid binding protein is required for efficient absorption and transport of bile acids in the distal portion of the murine small intestine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3519535/
https://www.ncbi.nlm.nih.gov/pubmed/23251388
http://dx.doi.org/10.1371/journal.pone.0050810
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